Restriction map for pF1KE0561
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# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AflII       AflIII      AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspHI       
AspI        AvaI        AvrII       BamHI       BbrPI       
BbvI        BcgI        BciVI       BcnI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BseAI       BseRI       BsiHKAI     BsiWI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
Eam1105I    EarI        Eco47III    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FseI        HgaI        
HpaI        KpnI        Ksp632I     KspI        MaeI        
MamI        MfeI        MflI        MluI        MroI        
MseI        MspA1I      MunI        MvnI        NaeI        
NciI        NdeI        NgoMIV      NheI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SmaI        SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tru9I       TseI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        


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# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccI          465  GTCTAC               GT^[AC][GT]AC
AcyI          227  GGCGCC               G[GA]^CG[CT]C
AfaI          757  GTAC                 GT^AC
AgeI          611  ACCGGT               A^CCGGT
AlwI          491  GATCC                GGATC(4/5)
AlwNI         982  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          371  CCTCAGG              CC^T[ACGT]AGG
Asp700I       738  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         626  TGCGCA               TGC^GCA
BalI          351  TGGCCA               TGG^CCA
BanII         328  GGGCTC               G[GA]GC[CT]^C
BbeI          227  GGCGCC               GGCGC^C
BbvCI         187  CCTCAGC              CCTCAGC(-5/-2)
BclI          693  TGATCA               T^GATCA
BglI          361  GCCACAGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          328  GGGCTC               G[AGT]GC[ACT]^C
BpmI          988  CTGGAG               CTGGAG(16/14)
BsaAI         932  TACGTA               [CT]AC^GT[GA]
BsaHI         227  GGCGCC               G[GA]^CG[CT]C
BsaI          976  GAGACC               GGTCTC(1/5)
BsaWI         611  ACCGGT               [AT]^CCGG[AT]
BsgI          567  GTGCAG               GTGCAG(16/14)
BsiEI         198  CGGCCG               CG[GA][CT]^CG
BsmBI         679  GAGACG               CGTCTC(1/5)
Bsp1286I      328  GGGCTC               G[AGT]GC[ACT]^C
BspHI         279  TCATGA               T^CATGA
BspMI         660  ACCTGC               ACCTGC(4/8)
BstAPI        642  GCATGGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         318  CCACCCTCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        371  CCTCAGG              CC^T[ACGT]AGG
DsaI          339  CCATGG               C^C[GA][CT]GG
EagI          198  CGGCCG               C^GGCCG
EclXI         198  CGGCCG               C^GGCCG
Eco52I        198  CGGCCG               C^GGCCG
Eco81I        371  CCTCAGG              CC^T[ACGT]AGG
EcoT14I       339  CCATGG               C^C[AT][AT]GG
FbaI          693  TGATCA               T^GATCA
FspI          626  TGCGCA               TGC^GCA
HaeII         227  GGCGCC               [GA]GCGC^[CT]
Hin1I         227  GGCGCC               G[GA]^CG[CT]C
HindIII       265  AAGCTT               A^AGCTT
KasI          227  GGCGCC               G^GCGCC
MluNI         351  TGGCCA               TGG^CCA
MscI          351  TGGCCA               TGG^CCA
NarI          227  GGCGCC               GG^CGCC
NcoI          339  CCATGG               C^CATGG
NotI          197  GCGGCCGC             GC^GGCCGC
PinAI         611  ACCGGT               A^CCGGT
PpuMI          20  AGGACCT              [GA]G^G[AT]CC[CT]
RcaI          279  TCATGA               T^CATGA
RsaI          757  GTAC                 GT^AC
SexAI         113  ACCTGGT              A^CC[AT]GGT
SfoI          227  GGCGCC               GGC^GCC
SnaBI         932  TACGTA               TAC^GTA
StuI          368  AGGCCT               AGG^CCT
StyI          339  CCATGG               C^C[AT][AT]GG
TaqI          489  TCGA                 T^CGA
TfiI          879  GAATC                G^A[AT]TC
Tsp45I        142  GTCAC                ^GT[GC]AC
Tsp509I       996  AATT                 ^AATT
TthHB8I       489  TCGA                 T^CGA
XcmI          339  CCATGGATGGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          738  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AluI          266  AGCT                 AG^CT
              986  AGCT                
BanI          227  GGCGCC               G^G[CT][GA]CC
              648  GGTGCC              
BbsI          180  GAAGAC               GAAGAC(2/6)
              273  GAAGAC              
BpuAI         180  GAAGAC               GAAGAC(2/6)
              273  GAAGAC              
BsmAI         679  GAGAC                GTCTC(1/5)
              976  GAGAC               
BsrFI         203  GCCGGT               [GA]^CCGG[CT]
              611  ACCGGT              
CfoI          228  GCGC                 GCG^C
              627  GCGC                
Cfr10I        203  GCCGGT               [GA]^CCGG[CT]
              611  ACCGGT              
EaeI          198  CGGCCG               [CT]^GGCC[GA]
              351  TGGCCA              
EcoNI         187  CCTCAGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              212  CCTGTGTGAGG         
HapII         204  CCGG                 C^CGG
              612  CCGG                
HhaI          228  GCGC                 GCG^C
              627  GCGC                
HinP1I        228  GCGC                 G^CGC
              627  GCGC                
HincII        103  GTCAAC               GT[CT]^[GA]AC
              724  GTCAAC              
HindII        103  GTCAAC               GT[CT]^[GA]AC
              724  GTCAAC              
HpaII         204  CCGG                 C^CGG
              612  CCGG                
MaeII         786  ACGT                 A^CGT
              933  ACGT                
MaeIII        142  GTCAC                ^GT[ACGT]AC
              835  GTAAC               
MslI          319  CACCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              540  CATTGTGGTG          
MspI          204  CCGG                 C^CGG
              612  CCGG                
PflMI         747  CCAGAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              981  CCAGGAGCTGG         
SmlI           25  CTCAAG               C^T[CT][GA]AG
              262  CTCAAG              
Van91I        747  CCAGAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              981  CCAGGAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   37 enzymes) #
#########################################

AciI        AvaII       BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MboI        MboII       MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TspRI       
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