Restriction map for pF1KE0561
 (Linear) (Six-base) MAPSORT of: KIEE0561.seq  Check: 1985  from: 1  to: 999



KIEE0561.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     466     999 
   Size:        466     533
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     292     622     999 
   Size:         51     241     330     377

  Fragments arranged by size: 

                 377     330     241      51
 
AgeI A'CCGG_T

Cuts at:      0     611     999 
   Size:        611     388
 
AlwNI CAG_nnn'CTG

Cuts at:      0     987     999 
   Size:        987      12
 
BanI G'GyrC_C

Cuts at:      0     227     648     999 
   Size:        227     421     351

  Fragments arranged by size: 

                 421     351     227
 
BanII G_rGCy'C

Cuts at:      0     332     999 
   Size:        332     667
 
BbeI G_GCGC'C

Cuts at:      0     231     999 
   Size:        231     768
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     187     280     999 
   Size:        187      93     719

  Fragments arranged by size: 

                 719     187      93
 
BbvCI CC'TCA_GC

Cuts at:      0     188     999 
   Size:        188     811
 
BclI T'GATC_A

Cuts at:      0     693     999 
   Size:        693     306
 
BglI GCCn_nnn'nGGC

Cuts at:      0     367     999 
   Size:        367     632
 
BmgBI CAC'GTC

Cuts at:      0     787     999 
   Size:        787     212
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     430     999 
   Size:        430     569
 
Bpu10I CC'TnA_GC

Cuts at:      0     188     999 
   Size:        188     811
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     247     999 
   Size:         10     237     752

  Fragments arranged by size: 

                 752     237      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     970     999 
   Size:        970      29
 
BsaAI yAC'GTr

Cuts at:      0     934     999 
   Size:        934      65
 
BsaHI Gr'CG_yC

Cuts at:      0     228     999 
   Size:        228     771
 
BsaWI w'CCGG_w

Cuts at:      0     611     999 
   Size:        611     388
 
BseYI C'CCAG_C

Cuts at:      0     292     999 
   Size:        292     707
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     588     999 
   Size:        588     411
 
BsiEI CG_ry'CG

Cuts at:      0     201     999 
   Size:        201     798
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     673     999 
   Size:        673     326
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     999 
   Size:        332     667
 
BspHI T'CATG_A

Cuts at:      0     279     999 
   Size:        279     720
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     669     999 
   Size:        669     330
 
BsrFI r'CCGG_y

Cuts at:      0     203     611     999 
   Size:        203     408     388

  Fragments arranged by size: 

                 408     388     203
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     648     999 
   Size:        648     351
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     325     999 
   Size:        325     674
 
Bsu36I CC'TnA_GG

Cuts at:      0     372     999 
   Size:        372     627
 
BtgI C'CryG_G

Cuts at:      0     339     999 
   Size:        339     660
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      78     113     999 
   Size:         78      35     886

  Fragments arranged by size: 

                 886      78      35
 
EaeI y'GGCC_r

Cuts at:      0     198     351     999 
   Size:        198     153     648

  Fragments arranged by size: 

                 648     198     153
 
EagI C'GGCC_G

Cuts at:      0     198     999 
   Size:        198     801
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     292     622     999 
   Size:         51     241     330     377

  Fragments arranged by size: 

                 377     330     241      51
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     191     216     999 
   Size:        191      25     783

  Fragments arranged by size: 

                 783     191      25
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21     473     810     999 
   Size:         21     452     337     189

  Fragments arranged by size: 

                 452     337     189      21
 
FspI TGC'GCA

Cuts at:      0     628     999 
   Size:        628     371
 
FspAI rTGC'GCAy

Cuts at:      0     628     999 
   Size:        628     371
 
HaeII r_GCGC'y

Cuts at:      0     231     999 
   Size:        231     768
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     864     896     999 
   Size:        864      32     103

  Fragments arranged by size: 

                 864     103      32
 
HincII GTy'rAC

Cuts at:      0     105     726     999 
   Size:        105     621     273

  Fragments arranged by size: 

                 621     273     105
 
HindIII A'AGCT_T

Cuts at:      0     265     999 
   Size:        265     734
 
KasI G'GCGC_C

Cuts at:      0     227     999 
   Size:        227     772
 
MscI TGG'CCA

Cuts at:      0     353     999 
   Size:        353     646
 
MslI CAynn'nnrTG

Cuts at:      0     323     544     999 
   Size:        323     221     455

  Fragments arranged by size: 

                 455     323     221
 
NarI GG'CG_CC

Cuts at:      0     228     999 
   Size:        228     771
 
NcoI C'CATG_G

Cuts at:      0     339     999 
   Size:        339     660
 
NotI GC'GGCC_GC

Cuts at:      0     198     999 
   Size:        198     801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     753     987     999 
   Size:        753     234      12

  Fragments arranged by size: 

                 753     234      12
 
PpuMI rG'GwC_Cy

Cuts at:      0      21     999 
   Size:         21     978
 
SexAI A'CCwGG_T

Cuts at:      0     113     999 
   Size:        113     886
 
SfcI C'TryA_G

Cuts at:      0     243     467     822     999 
   Size:        243     224     355     177

  Fragments arranged by size: 

                 355     243     224     177
 
SfoI GGC'GCC

Cuts at:      0     229     999 
   Size:        229     770
 
SmlI C'TyrA_G

Cuts at:      0      25     262     999 
   Size:         25     237     737

  Fragments arranged by size: 

                 737     237      25
 
SnaBI TAC'GTA

Cuts at:      0     934     999 
   Size:        934      65
 
StuI AGG'CCT

Cuts at:      0     370     999 
   Size:        370     629
 
StyI C'CwwG_G

Cuts at:      0     339     999 
   Size:        339     660
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     346     999 
   Size:        346     653
 
XmnI GAAnn'nnTTC

Cuts at:      0     742     999 
   Size:        742     257
 

 Enzymes that do cut:

     AccI     AcuI     AgeI    AlwNI     BanI    BanII     BbeI     BbsI
    BbvCI     BclI     BglI    BmgBI     BmrI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsmBI Bsp1286I
    BspHI    BspMI    BsrFI   BstAPI    BstXI   Bsu36I     BtgI    CspCI
     EaeI     EagI  Eco57MI    EcoNI EcoO109I     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     KasI     MscI     MslI     NarI     NcoI
     NotI    PflMI    PpuMI    SexAI     SfcI     SfoI     SmlI    SnaBI
     StuI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BcgI    BciVI    BfrBI
    BglII     BlpI Bme1580I     BmtI     BplI     BpmI    BsaBI    BsaXI
    BseRI  BsiHKAI    BsiWI     BsmI    BspEI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     BtsI
     ClaI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     HpaI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI
     SgfI    SgrAI     SmaI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
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