Restriction map for pF1KA0749
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

Acc65I      AccIII      AclI        AcsI        AfaI        
AflII       AgeI        Alw44I      Aor51HI     ApaLI       
ApoI        AseI        AsnI        Asp718I     AviII       
AvrII       BalI        BclI        BfrI        BlnI        
BsaBI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstZ17I     ClaI        
CpoI        DraI        DraIII      DrdI        Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
MamI        MfeI        MluNI       MroI        MscI        
MseI        MslI        MunI        NcoI        NdeI        
NheI        NlaIII      NotI        NruI        NsiI        
NspI        NspV        PacI        PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tsp509I     XbaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII         676  GACGTC               GACGT^C
AccI          260  GTCGAC               GT^[AC][GT]AC
AhdI          388  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1553  CCTTAGG              CC^T[ACGT]AGG
AscI         1619  GGCGCGCC             GG^CGCGCC
Asp700I      1826  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         388  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1870  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI         1851  GAAGAC               GAAGAC(2/6)
BcgI          767  GCAAGGGGGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1688  GTATCC               GTATCC(6/5)
BfaI         1888  CTAG                 C^TAG
BglII         974  AGATCT               A^GATCT
BlpI         1737  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1737  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1851  GAAGAC               GAAGAC(2/6)
BsgI         1261  GTGCAG               GTGCAG(16/14)
BssHII       1620  GCGCGC               G^CGCGC
BstAPI       1263  GCAGCTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I       1553  CCTTAGG              CC^T[ACGT]AGG
CelII        1737  GCTCAGC              GC^T[ACGT]AGC
Eam1105I      388  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1553  CCTTAGG              CC^T[ACGT]AGG
HincII        260  GTCGAC               GT[CT]^[GA]AC
HindII        260  GTCGAC               GT[CT]^[GA]AC
KspI         1416  CCGCGG               CCGC^GG
MaeI         1888  CTAG                 C^TAG
MaeIII       1282  GTGAC                ^GT[ACGT]AC
MluI         1287  ACGCGT               A^CGCGT
PaeR7I        948  CTCGAG               C^TCGAG
PflFI        1870  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          109  CTGCAG               CTGCA^G
SacII        1416  CCGCGG               CCGC^GG
SalI          260  GTCGAC               G^TCGAC
Tsp45I       1282  GTGAC                ^GT[GC]AC
Tth111I      1870  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          948  CTCGAG               C^TCGAG
XmnI         1826  GAAACAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AflIII         46  ACGTGT               A^C[GA][CT]GT
             1287  ACGCGT              
BbeI          342  GGCGCC               GGCGC^C
             1786  GGCGCC              
BbrPI          45  CACGTG               CAC^GTG
             1209  CACGTG              
BbvCI        1429  GCTGAGG              CCTCAGC(-5/-2)
             1729  GCTGAGG             
BsaWI        1066  ACCGGA               [AT]^CCGG[AT]
             1107  ACCGGA              
EcoNI         141  CCTGGCCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1812  CCTGCCCGAGG         
EcoT14I       203  CCAAGG               C^C[AT][AT]GG
              554  CCTTGG              
KasI          342  GGCGCC               G^GCGCC
             1786  GGCGCC              
NaeI           86  GCCGGC               GCC^GGC
             1624  GCCGGC              
NarI          342  GGCGCC               GG^CGCC
             1786  GGCGCC              
NgoMIV         86  GCCGGC               G^CCGGC
             1624  GCCGGC              
PmaCI          45  CACGTG               CAC^GTG
             1209  CACGTG              
PmlI           45  CACGTG               CAC^GTG
             1209  CACGTG              
PvuII         301  CAGCTG               CAG^CTG
              608  CAGCTG              
SacI          155  GAGCTC               GAGCT^C
             1735  GAGCTC              
SfaNI         912  GCATC                GCATC(5/9)
             1478  GCATC               
SfcI          109  CTGCAG               C^T[GA][CT]AG
              848  CTACAG              
SfoI          342  GGCGCC               GGC^GCC
             1786  GGCGCC              
SmlI          940  CTCAAG               C^T[CT][GA]AG
              948  CTCGAG              
StyI          203  CCAAGG               C^C[AT][AT]GG
              554  CCTTGG              
XcmI          391  CCAGGGTCGGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              487  CCACGACGCCGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AlwI        
AlwNI       ApaI        AspHI       AvaI        AvaII       
BamHI       BanI        BanII       BbvI        BcnI        
BglI        BmyI        BpmI        BsaAI       BsaHI       
BsaI        BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeII       MboI        MboII       MflI        MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIV       PflMI       PleI        
PpuMI       Sau3AI      Sau96I      ScrFI       SmaI        
TaqI        TfiI        TseI        TspRI       TthHB8I     
Van91I      XhoII       XmaCI       XmaI        
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