Restriction map for pF1KB6108
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BcgI        BciVI       BfrI        BglII       
BlnI        BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsoBI       
BspDI       BspEI       BspHI       BsrDI       BssHII      
BstBI       BstEII      BstPI       BstYI       Bsu36I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MflI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII          40  CGCG                 CG^CG
AflIII        715  ACATGT               A^C[GA][CT]GT
Asp700I      1059  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI          101  TGGCCA               TGG^CCA
BbsI          839  GAAGAC               GAAGAC(2/6)
BclI         1712  TGATCA               T^GATCA
BcnI         1698  CCCGG                CC^[GC]GG
BglI            7  GCCGTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         839  GAAGAC               GAAGAC(2/6)
BsaI         1004  GGTCTC               GGTCTC(1/5)
BsmBI        1562  CGTCTC               CGTCTC(1/5)
BspLU11I      715  ACATGT               A^CATGT
BsrBI        1626  GAGCGG               CCGCTC(-3/-3)
BssSI        1028  CTCGTG               CACGAG(-5/-1)
Bst1107I      632  GTATAC               GTA^TAC
BstAPI       1386  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I       1542  CATCC                GGATG(2/0)
BstUI          40  CGCG                 CG^CG
BstXI         104  CCAGCCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       632  GTATAC               GTA^TAC
DraIII        516  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI        1639  CCTGTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         1712  TGATCA               T^GATCA
FokI         1542  CATCC                GGATG(9/13)
HaeII          65  AGCGCC               [GA]GCGC^[CT]
MaeII          98  ACGT                 A^CGT
MluNI         101  TGGCCA               TGG^CCA
MscI          101  TGGCCA               TGG^CCA
MseI         1789  TTAA                 T^TAA
MvnI           40  CGCG                 CG^CG
NciI         1698  CCCGG                CC^[GC]GG
NcoI         1580  CCATGG               C^CATGG
PstI           61  CTGCAG               CTGCA^G
SphI         1395  GCATGC               GCATG^C
StuI         1214  AGGCCT               AGG^CCT
TaqI         1067  TCGA                 T^CGA
Tru9I        1789  TTAA                 T^TAA
TthHB8I      1067  TCGA                 T^CGA
XcmI          414  CCATCAGGGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1059  GAACCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          475  GTAGAC               GT^[AC][GT]AC
              632  GTATAC              
AlwI          447  GATCC                GGATC(4/5)
             1478  GGATC               
AvaII         288  GGACC                G^G[AT]CC
              988  GGACC               
BbvCI         637  CCTCAGC              CCTCAGC(-5/-2)
             1534  CCTCAGC             
BfaI           52  CTAG                 C^TAG
             1112  CTAG                
BsmAI        1005  GTCTC                GTCTC(1/5)
             1563  GTCTC               
BsmFI        1575  GTCCC                GGGAC(10/14)
             1606  GTCCC               
BsmI          355  GAATGC               GAATGC(1/-1)
              488  GAATGC              
BspMI        1125  ACCTGC               ACCTGC(4/8)
             1659  ACCTGC              
BsrFI         938  ACCGGC               [GA]^CCGG[CT]
             1406  ACCGGC              
Cfr10I        938  ACCGGC               [GA]^CCGG[CT]
             1406  ACCGGC              
DraII         287  AGGACCC              [GA]G^G[ACGT]CC[CT]
              987  AGGACCT             
DsaI            8  CCGTGG               C^C[GA][CT]GG
             1580  CCATGG              
EarI         1813  CTCTTC               CTCTTC(1/4)
             1268  GAAGAG              
EcoO109I      287  AGGACCC              [GA]G^G[ACGT]CC[CT]
              987  AGGACCT             
FauI         1608  CCCGC                CCCGC(4/6)
              970  GCGGG               
HindIII       787  AAGCTT               A^AGCTT
             1363  AAGCTT              
Ksp632I      1813  CTCTTC               CTCTTC(1/4)
             1268  GAAGAG              
MaeI           52  CTAG                 C^TAG
             1112  CTAG                
MslI          216  CACCATTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1173  CACCCCTGTG          
MspA1I       1136  CAGCTG               C[AC]G^C[GT]G
             1390  CAGCTG              
NspI          715  ACATGT               [GA]CATG^[CT]
             1395  GCATGC              
PflMI         140  CCAACGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              202  CCAAAGCATGG         
PpuMI         287  AGGACCC              [GA]G^G[AT]CC[CT]
              987  AGGACCT             
PvuII        1136  CAGCTG               CAG^CTG
             1390  CAGCTG              
SacI         1157  GAGCTC               GAGCT^C
             1446  GAGCTC              
SfaNI          89  GCATC                GCATC(5/9)
             1541  GCATC               
SmlI         1346  CTCAAG               C^T[CT][GA]AG
             1826  CTCAAG              
TfiI          212  GATTC                G^A[AT]TC
             1471  GAATC               
Tsp45I       1106  GTGAC                ^GT[GC]AC
             1171  GTCAC               
Van91I        140  CCAACGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              202  CCAAAGCATGG         


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwNI       
ApoI        AspHI       BanII       BbvI        BlpI        
BmyI        BpmI        Bpu1102I    BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        Bsp1286I    Bsp1407I    
BsrGI       BsrI        BssKI       BstNI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EcoRII      EcoT14I     Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SspBI       StyI        
TseI        Tsp509I     TspRI       
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