Restriction map for pF1KB6108
 (Linear) (Six-base) MAPSORT of: KIBB6108.seq  Check: 5947  from: 1  to: 1851



KIBB6108.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:45  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1134    1668    1851 
   Size:       1134     534     183

  Fragments arranged by size: 

                1134     534     183
 
AccI GT'mk_AC

Cuts at:      0     476     633    1851 
   Size:        476     157    1218

  Fragments arranged by size: 

                1218     476     157
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1060    1351    1378    1800    1851 
   Size:       1060     291      27     422      51

  Fragments arranged by size: 

                1060     422     291      51      27
 
AflIII A'CryG_T

Cuts at:      0     715    1851 
   Size:        715    1136
 
AleI CACnn'nnGTG

Cuts at:      0    1177    1851 
   Size:       1177     674
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1412    1851 
   Size:       1412     439
 
AlwNI CAG_nnn'CTG

Cuts at:      0     991    1062    1126    1392    1851 
   Size:        991      71      64     266     459

  Fragments arranged by size: 

                 991     459     266      71      64
 
ApoI r'AATT_y

Cuts at:      0     332     434     976    1851 
   Size:        332     102     542     875

  Fragments arranged by size: 

                 875     542     332     102
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1744    1777    1851 
   Size:       1744      33      74

  Fragments arranged by size: 

                1744      74      33
 
BanII G_rGCy'C

Cuts at:      0    1161    1450    1699    1706    1851 
   Size:       1161     289     249       7     145

  Fragments arranged by size: 

                1161     289     249     145       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     846    1851 
   Size:        846    1005
 
BbvCI CC'TCA_GC

Cuts at:      0     638    1535    1851 
   Size:        638     897     316

  Fragments arranged by size: 

                 897     638     316
 
BclI T'GATC_A

Cuts at:      0    1712    1851 
   Size:       1712     139
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13    1851 
   Size:         13    1838
 
BlpI GC'TnA_GC

Cuts at:      0     232    1134    1202    1851 
   Size:        232     902      68     649

  Fragments arranged by size: 

                 902     649     232      68
 
BmgBI CAC'GTC

Cuts at:      0      99    1851 
   Size:         99    1752
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     436     605    1851 
   Size:        436     169    1246

  Fragments arranged by size: 

                1246     436     169
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     446    1169    1352    1723    1729    1851 
   Size:        268     178     723     183     371       6     122

  Fragments arranged by size: 

                 723     371     268     183     178     122       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     638    1535    1851 
   Size:        638     897     316

  Fragments arranged by size: 

                 897     638     316
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1331    1811    1851 
   Size:       1331     480      40

  Fragments arranged by size: 

                1331     480      40
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1010    1851 
   Size:       1010     841
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1283    1313    1542    1572    1851 
   Size:       1283      30     229      30     279

  Fragments arranged by size: 

                1283     279     229      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     998    1502    1669    1851 
   Size:        998     504     167     182

  Fragments arranged by size: 

                 998     504     182     167
 
BsiHKAI G_wGCw'C

Cuts at:      0    1161    1439    1450    1851 
   Size:       1161     278      11     401

  Fragments arranged by size: 

                1161     401     278      11
 
BsmI GAATG_Cn'

Cuts at:      0     361     494    1851 
   Size:        361     133    1357

  Fragments arranged by size: 

                1357     361     133
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1568    1851 
   Size:       1568     283
 
Bsp1286I G_dGCh'C

Cuts at:      0    1161    1439    1450    1699    1706    1851 
   Size:       1161     278      11     249       7     145

  Fragments arranged by size: 

                1161     278     249     145      11       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1134    1668    1851 
   Size:       1134     534     183

  Fragments arranged by size: 

                1134     534     183
 
BsrBI CCG'CTC

Cuts at:      0    1628    1851 
   Size:       1628     223
 
BsrFI r'CCGG_y

Cuts at:      0     938    1406    1851 
   Size:        938     468     445

  Fragments arranged by size: 

                 938     468     445
 
BsrGI T'GTAC_A

Cuts at:      0     119    1035    1374    1851 
   Size:        119     916     339     477

  Fragments arranged by size: 

                 916     477     339     119
 
BssSI C'ACGA_G

Cuts at:      0    1028    1851 
   Size:       1028     823
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1392    1851 
   Size:       1392     459
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     111    1851 
   Size:        111    1740
 
BstZ17I GTA'TAC

Cuts at:      0     634    1851 
   Size:        634    1217
 
BtgI C'CryG_G

Cuts at:      0       8    1580    1851 
   Size:          8    1572     271

  Fragments arranged by size: 

                1572     271       8
 
BtsI GCAGTG_nn'

Cuts at:      0    1753    1851 
   Size:       1753      98
 
DraIII CAC_nnn'GTG

Cuts at:      0     521    1851 
   Size:        521    1330
 
EaeI y'GGCC_r

Cuts at:      0     101     129    1408    1418    1851 
   Size:        101      28    1279      10     433

  Fragments arranged by size: 

                1279     433     101      28      10
 
EarI CTCTTCn'nnn_

Cuts at:      0    1263    1819    1851 
   Size:       1263     556      32

  Fragments arranged by size: 

                1263     556      32
 
EcoICRI GAG'CTC

Cuts at:      0    1159    1448    1851 
   Size:       1159     289     403

  Fragments arranged by size: 

                1159     403     289
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     268     446    1060    1169    1351    1352    1378    1723 
   Size:        268     178     614     109     182       1      26     345

Cuts at:   1723    1729    1800    1851 
   Size:          6      71      51

  Fragments arranged by size: 

                 614     345     268     182     178     109      71      51
                  26       6       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1643    1851 
   Size:       1643     208
 
EcoO109I rG'GnC_Cy

Cuts at:      0     288     988    1851 
   Size:        288     700     863

  Fragments arranged by size: 

                 863     700     288
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     212     805    1851 
   Size:        212     593    1046

  Fragments arranged by size: 

                1046     593     212
 
HaeII r_GCGC'y

Cuts at:      0      69    1851 
   Size:         69    1782
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     308     340    1283    1315    1329    1361    1851 
   Size:        308      32     943      32      14      32     490

  Fragments arranged by size: 

                 943     490     308      32      32      32      14
 
HindIII A'AGCT_T

Cuts at:      0     787    1363    1851 
   Size:        787     576     488

  Fragments arranged by size: 

                 787     576     488
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     303    1851 
   Size:        303    1548
 
MscI TGG'CCA

Cuts at:      0     103    1851 
   Size:        103    1748
 
MslI CAynn'nnrTG

Cuts at:      0     220    1177    1851 
   Size:        220     957     674

  Fragments arranged by size: 

                 957     674     220
 
MspA1I CmG'CkG

Cuts at:      0    1138    1392    1851 
   Size:       1138     254     459

  Fragments arranged by size: 

                1138     459     254
 
NcoI C'CATG_G

Cuts at:      0    1580    1851 
   Size:       1580     271
 
NspI r_CATG'y

Cuts at:      0     719    1399    1851 
   Size:        719     680     452

  Fragments arranged by size: 

                 719     680     452
 
PasI CC'CwG_GG

Cuts at:      0    1087    1851 
   Size:       1087     764
 
PciI A'CATG_T

Cuts at:      0     715    1851 
   Size:        715    1136
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     146     208    1851 
   Size:        146      62    1643

  Fragments arranged by size: 

                1643     146      62
 
PfoI T'CCnGG_A

Cuts at:      0     284    1185    1474    1745    1851 
   Size:        284     901     289     271     106

  Fragments arranged by size: 

                 901     289     284     271     106
 
PpuMI rG'GwC_Cy

Cuts at:      0     288     988    1851 
   Size:        288     700     863

  Fragments arranged by size: 

                 863     700     288
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     516     548    1851 
   Size:        516      32    1303

  Fragments arranged by size: 

                1303     516      32
 
PstI C_TGCA'G

Cuts at:      0      65    1851 
   Size:         65    1786
 
PvuII CAG'CTG

Cuts at:      0    1138    1392    1851 
   Size:       1138     254     459

  Fragments arranged by size: 

                1138     459     254
 
SacI G_AGCT'C

Cuts at:      0    1161    1450    1851 
   Size:       1161     289     401

  Fragments arranged by size: 

                1161     401     289
 
SfcI C'TryA_G

Cuts at:      0      61     303    1550    1851 
   Size:         61     242    1247     301

  Fragments arranged by size: 

                1247     301     242      61
 
SmlI C'TyrA_G

Cuts at:      0    1346    1826    1851 
   Size:       1346     480      25

  Fragments arranged by size: 

                1346     480      25
 
SphI G_CATG'C

Cuts at:      0    1399    1851 
   Size:       1399     452
 
StuI AGG'CCT

Cuts at:      0    1216    1851 
   Size:       1216     635
 
StyI C'CwwG_G

Cuts at:      0    1415    1466    1580    1851 
   Size:       1415      51     114     271

  Fragments arranged by size: 

                1415     271     114      51
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     929    1506    1851 
   Size:        929     577     345

  Fragments arranged by size: 

                 929     577     345
 
TatI w'GTAC_w

Cuts at:      0     119    1035    1114    1374    1851 
   Size:        119     916      79     260     477

  Fragments arranged by size: 

                 916     477     260     119      79
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     421    1851 
   Size:        421    1430
 
XmnI GAAnn'nnTTC

Cuts at:      0    1063    1851 
   Size:       1063     788
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AleI     AlfI    AlwNI     ApoI
     BaeI    BanII     BbsI    BbvCI     BclI     BglI     BlpI    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BsrGI    BssSI
   BstAPI    BstXI  BstZ17I     BtgI     BtsI   DraIII     EaeI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I  HindIII
     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PasI     PciI
    PflMI     PfoI    PpuMI     PsrI     PstI    PvuII     SacI     SfcI
     SmlI     SphI     StuI     StyI    TaqII     TatI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI     BbeI     BcgI    BciVI    BfrBI    BglII Bme1580I     BmtI
     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
    BsiWI    BspEI    BspHI    BsrDI   BssHII    BstBI   BstEII    BstYI
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI
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