Restriction map for pF1KB7183
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
BamHI       BbsI        BcgI        BclI        BfrI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HinfI       
HpaI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PleI        PmeI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
ScaI        SexAI       SfuI        SgrAI       SnaBI       
SpeI        SspBI       SspI        SwaI        TaqI        
TfiI        Tsp509I     Tth111I     TthHB8I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII         559  GACGTC               GACGT^C
AccI         1450  GTCTAC               GT^[AC][GT]AC
Aor51HI      1277  AGCGCT               AGC^GCT
Asp700I      1404  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         253  GGACC                G^G[AT]CC
AvrII          94  CCTAGG               C^CTAGG
BanI         1358  GGCGCC               G^G[CT][GA]CC
BbeI         1358  GGCGCC               GGCGC^C
BbvCI         147  GCTGAGG              CCTCAGC(-5/-2)
BciVI         809  GTATCC               GTATCC(6/5)
BglII         161  AGATCT               A^GATCT
BlnI           94  CCTAGG               C^CTAGG
BsaI         1111  GAGACC               GGTCTC(1/5)
BseRI        1486  CTCCTC               GAGGAG(10/8)
BspMI         188  ACCTGC               ACCTGC(4/8)
BsrDI        1235  GCAATG               GCAATG(2/0)
BstXI         917  CCAACTCTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III     1277  AGCGCT               AGC^GCT
FauI         1095  GCGGG                CCCGC(4/6)
HgaI          975  GACGC                GACGC(5/10)
KasI         1358  GGCGCC               G^GCGCC
MseI         1505  TTAA                 T^TAA
MspA1I       1193  CAGCTG               C[AC]G^C[GT]G
NarI         1358  GGCGCC               GG^CGCC
NspI         1337  GCATGC               [GA]CATG^[CT]
PvuII        1193  CAGCTG               CAG^CTG
SbfI          323  CCTGCAGG             CCTGCA^GG
SfiI          628  GGCCTGGTTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1358  GGCGCC               GGC^GCC
SmaI          539  CCCGGG               CCC^GGG
SphI         1337  GCATGC               GCATG^C
Sse8387I      323  CCTGCAGG             CCTGCA^GG
Tru9I        1505  TTAA                 T^TAA
XmaCI         539  CCCGGG               C^CCGGG
XmaI          539  CCCGGG               C^CCGGG
XmnI         1404  GAAGCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          559  GACGTC               G[GA]^CG[CT]C
             1358  GGCGCC              
ApaI           89  GGGCCC               GGGCC^C
              226  GGGCCC              
BalI          491  TGGCCA               TGG^CCA
              636  TGGCCA              
BbrPI        1318  CACGTG               CAC^GTG
             1396  CACGTG              
BcnI          539  CCCGG                CC^[GC]GG
              540  CCGGG               
BsaAI        1318  CACGTG               [CT]AC^GT[GA]
             1396  CACGTG              
BsaHI         559  GACGTC               G[GA]^CG[CT]C
             1358  GGCGCC              
BstAPI       1011  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1392  GCACCACGTGC         
BstYI         161  AGATCT               [GA]^GATC[CT]
             1013  AGATCC              
DraIII       1315  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1393  CACCACGTG           
Hin1I         559  GACGTC               G[GA]^CG[CT]C
             1358  GGCGCC              
HphI          799  GGTGA                GGTGA(8/7)
             1155  GGTGA               
MaeIII        800  GTGAC                ^GT[ACGT]AC
              897  GTCAC               
MflI          161  AGATCT               [GA]^GATC[CT]
             1013  AGATCC              
MluNI         491  TGGCCA               TGG^CCA
              636  TGGCCA              
MscI          491  TGGCCA               TGG^CCA
              636  TGGCCA              
NciI          539  CCCGG                CC^[GC]GG
              540  CCGGG               
PmaCI        1318  CACGTG               CAC^GTG
             1396  CACGTG              
PmlI         1318  CACGTG               CAC^GTG
             1396  CACGTG              
SacI          874  GAGCTC               GAGCT^C
             1158  GAGCTC              
SmlI         1105  CTCAAG               C^T[CT][GA]AG
             1430  CTCAAG              
Tsp45I        800  GTGAC                ^GT[GC]AC
              897  GTCAC               
XhoII         161  AGATCT               [GA]^GATC[CT]
             1013  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        BanII       BbvI        BfaI        
BglI        BmyI        BpmI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EcoNI       
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MaeI        MaeII       MboI        
MboII       MnlI        MslI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PflMI       
PstI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StuI        StyI        TseI        
TspRI       Van91I      XcmI        
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