Restriction map for pF1KB7183
 (Linear) (Six-base) MAPSORT of: KIBB7183.seq  Check: 7999  from: 1  to: 1509



KIBB7183.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     197    1509 
   Size:        197    1312
 
AatII G_ACGT'C

Cuts at:      0     563    1509 
   Size:        563     946
 
AccI GT'mk_AC

Cuts at:      0    1451    1509 
   Size:       1451      58
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     535    1159    1423    1509 
   Size:        535     624     264      86

  Fragments arranged by size: 

                 624     535     264      86
 
AfeI AGC'GCT

Cuts at:      0    1279    1509 
   Size:       1279     230
 
AleI CACnn'nnGTG

Cuts at:      0     377    1509 
   Size:        377    1132
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      42    1509 
   Size:         42    1467
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     673     705    1509 
   Size:        673      32     804

  Fragments arranged by size: 

                 804     673      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     222     513     831    1017    1509 
   Size:        222     291     318     186     492

  Fragments arranged by size: 

                 492     318     291     222     186
 
ApaI G_GGCC'C

Cuts at:      0      93     230    1509 
   Size:         93     137    1279

  Fragments arranged by size: 

                1279     137      93
 
AvaI C'yCGr_G

Cuts at:      0      86     539    1251    1351    1509 
   Size:         86     453     712     100     158

  Fragments arranged by size: 

                 712     453     158     100      86
 
AvrII C'CTAG_G

Cuts at:      0      94    1509 
   Size:         94    1415
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     792     825    1509 
   Size:        792      33     684

  Fragments arranged by size: 

                 792     684      33
 
BanI G'GyrC_C

Cuts at:      0    1358    1509 
   Size:       1358     151
 
BanII G_rGCy'C

Cuts at:      0      93     230     344     878    1162    1300    1509 
   Size:         93     137     114     534     284     138     209

  Fragments arranged by size: 

                 534     284     209     138     137     114      93
 
BbeI G_GCGC'C

Cuts at:      0    1362    1509 
   Size:       1362     147
 
BbvCI CC'TCA_GC

Cuts at:      0     148    1509 
   Size:        148    1361
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     820    1509 
   Size:        820     689
 
BglI GCCn_nnn'nGGC

Cuts at:      0      88     408     635     913    1509 
   Size:         88     320     227     278     596

  Fragments arranged by size: 

                 596     320     278     227      88
 
BglII A'GATC_T

Cuts at:      0     161    1509 
   Size:        161    1348
 
Bme1580I G_kGCm'C

Cuts at:      0      63      69      76      93     230     374    1325    1509 
   Size:         63       6       7      17     137     144     951     184

  Fragments arranged by size: 

                 951     184     144     137      63      17       7       6
            
 
BmgBI CAC'GTC

Cuts at:      0    1005    1478    1509 
   Size:       1005     473      31

  Fragments arranged by size: 

                1005     473      31
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      79     641    1509 
   Size:         79     562     868

  Fragments arranged by size: 

                 868     562      79
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     749    1509 
   Size:        749     760
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     217     358     700    1509 
   Size:        186      31     141     342     809

  Fragments arranged by size: 

                 809     342     186     141      31
 
Bpu10I CC'TnA_GC

Cuts at:      0     148    1259    1509 
   Size:        148    1111     250

  Fragments arranged by size: 

                1111     250     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1090    1415    1509 
   Size:       1090     325      94

  Fragments arranged by size: 

                1090     325      94
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1105    1509 
   Size:       1105     404
 
BsaAI yAC'GTr

Cuts at:      0    1320    1398    1509 
   Size:       1320      78     111

  Fragments arranged by size: 

                1320     111      78
 
BsaHI Gr'CG_yC

Cuts at:      0     560    1359    1509 
   Size:        560     799     150

  Fragments arranged by size: 

                 799     560     150
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     673     703     865     895    1509 
   Size:        673      30     162      30     614

  Fragments arranged by size: 

                 673     614     162      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1477    1509 
   Size:       1477      32
 
BseYI C'CCAG_C

Cuts at:      0     125     222     568     624    1191    1195    1227    1274 
   Size:        125      97     346      56     567       4      32      47

Cuts at:   1274    1509 
   Size:        235

  Fragments arranged by size: 

                 567     346     235     125      97      56      47      32
                   4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     481    1027    1375    1509 
   Size:        175     306     546     348     134

  Fragments arranged by size: 

                 546     348     306     175     134
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     767     878    1162    1509 
   Size:        188     579     111     284     347

  Fragments arranged by size: 

                 579     347     284     188     111
 
Bsp1286I G_dGCh'C

Cuts at:      0      63      69      76      93     188     230     344     374 
   Size:         63       6       7      17      95      42     114      30

Cuts at:    374     767     878    1162    1300    1325    1509 
   Size:        393     111     284     138      25     184

  Fragments arranged by size: 

                 393     284     184     138     114     111      95      63
                  42      30      25      17       7       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     197    1509 
   Size:        197    1312
 
BsrBI CCG'CTC

Cuts at:      0      85     621    1263    1509 
   Size:         85     536     642     246

  Fragments arranged by size: 

                 642     536     246      85
 
BsrDI GCAATG_nn'

Cuts at:      0    1242    1509 
   Size:       1242     267
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1017    1398    1509 
   Size:       1017     381     111

  Fragments arranged by size: 

                1017     381     111
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     924    1509 
   Size:        924     585
 
BstYI r'GATC_y

Cuts at:      0     161    1013    1509 
   Size:        161     852     496

  Fragments arranged by size: 

                 852     496     161
 
BtsI GCAGTG_nn'

Cuts at:      0     494     932    1509 
   Size:        494     438     577

  Fragments arranged by size: 

                 577     494     438
 
DraIII CAC_nnn'GTG

Cuts at:      0    1320    1398    1509 
   Size:       1320      78     111

  Fragments arranged by size: 

                1320     111      78
 
EaeI y'GGCC_r

Cuts at:      0     491     636    1034    1509 
   Size:        491     145     398     475

  Fragments arranged by size: 

                 491     475     398     145
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     886     904    1509 
   Size:        886      18     605

  Fragments arranged by size: 

                 886     605      18
 
EcoICRI GAG'CTC

Cuts at:      0     876    1160    1509 
   Size:        876     284     349

  Fragments arranged by size: 

                 876     349     284
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     186     217     358     535     700    1159    1423    1509 
   Size:        186      31     141     177     165     459     264      86

  Fragments arranged by size: 

                 459     264     186     177     165     141      86      31
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     883    1021    1366    1509 
   Size:        883     138     345     143

  Fragments arranged by size: 

                 883     345     143     138
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     226     510    1509 
   Size:         90     136     284     999

  Fragments arranged by size: 

                 999     284     136      90
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     783    1131    1509 
   Size:        783     348     378

  Fragments arranged by size: 

                 783     378     348
 
HaeII r_GCGC'y

Cuts at:      0      40     414    1281    1362    1509 
   Size:         40     374     867      81     147

  Fragments arranged by size: 

                 867     374     147      81      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     430     462    1413    1445    1509 
   Size:        430      32     951      32      64

  Fragments arranged by size: 

                 951     430      64      32      32
 
KasI G'GCGC_C

Cuts at:      0    1358    1509 
   Size:       1358     151
 
MscI TGG'CCA

Cuts at:      0     493     638    1509 
   Size:        493     145     871

  Fragments arranged by size: 

                 871     493     145
 
MslI CAynn'nnrTG

Cuts at:      0     377     715    1444    1509 
   Size:        377     338     729      65

  Fragments arranged by size: 

                 729     377     338      65
 
MspA1I CmG'CkG

Cuts at:      0    1195    1509 
   Size:       1195     314
 
NarI GG'CG_CC

Cuts at:      0    1359    1509 
   Size:       1359     150
 
NspI r_CATG'y

Cuts at:      0    1341    1509 
   Size:       1341     168
 
PasI CC'CwG_GG

Cuts at:      0     344    1509 
   Size:        344    1165
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222     678     755     831    1509 
   Size:        222     456      77      76     678

  Fragments arranged by size: 

                 678     456     222      77      76
 
PfoI T'CCnGG_A

Cuts at:      0     215     335    1509 
   Size:        215     120    1174

  Fragments arranged by size: 

                1174     215     120
 
PmlI CAC'GTG

Cuts at:      0    1320    1398    1509 
   Size:       1320      78     111

  Fragments arranged by size: 

                1320     111      78
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     673     705    1509 
   Size:        673      32     804

  Fragments arranged by size: 

                 804     673      32
 
PspOMI G'GGCC_C

Cuts at:      0      89     226    1509 
   Size:         89     137    1283

  Fragments arranged by size: 

                1283     137      89
 
PstI C_TGCA'G

Cuts at:      0     161     328     533     939    1509 
   Size:        161     167     205     406     570

  Fragments arranged by size: 

                 570     406     205     167     161
 
PvuII CAG'CTG

Cuts at:      0    1195    1509 
   Size:       1195     314
 
SacI G_AGCT'C

Cuts at:      0     878    1162    1509 
   Size:        878     284     347

  Fragments arranged by size: 

                 878     347     284
 
SbfI CC_TGCA'GG

Cuts at:      0     328    1509 
   Size:        328    1181
 
SfcI C'TryA_G

Cuts at:      0     157     324     354     529     935    1452    1509 
   Size:        157     167      30     175     406     517      57

  Fragments arranged by size: 

                 517     406     175     167     157      57      30
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     635    1509 
   Size:        635     874
 
SfoI GGC'GCC

Cuts at:      0    1360    1509 
   Size:       1360     149
 
SmaI CCC'GGG

Cuts at:      0     541    1509 
   Size:        541     968
 
SmlI C'TyrA_G

Cuts at:      0    1105    1430    1509 
   Size:       1105     325      79

  Fragments arranged by size: 

                1105     325      79
 
SphI G_CATG'C

Cuts at:      0    1341    1509 
   Size:       1341     168
 
StuI AGG'CCT

Cuts at:      0     775    1257    1470    1509 
   Size:        775     482     213      39

  Fragments arranged by size: 

                 775     482     213      39
 
StyI C'CwwG_G

Cuts at:      0      94     452     749    1509 
   Size:         94     358     297     760

  Fragments arranged by size: 

                 760     358     297      94
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     336    1509 
   Size:        336    1173
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     140    1192    1324    1509 
   Size:        140    1052     132     185

  Fragments arranged by size: 

                1052     185     140     132
 
XmaI C'CCGG_G

Cuts at:      0     539    1509 
   Size:        539     970
 
XmnI GAAnn'nnTTC

Cuts at:      0    1408    1509 
   Size:       1408     101
 
ZraI GAC'GTC

Cuts at:      0     561    1509 
   Size:        561     948
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AfeI     AleI     AlfI     AloI
    AlwNI     ApaI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI    BciVI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI   BstAPI
    BstXI    BstYI     BtsI   DraIII     EaeI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MscI     MslI
   MspA1I     NarI     NspI     PasI    PflMI     PfoI     PmlI     PpiI
   PspOMI     PstI    PvuII     SacI     SbfI     SfcI     SfiI     SfoI
     SmaI     SmlI     SphI     StuI     StyI    TaqII     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI    AflII   AflIII     AgeI     AhdI    ApaLI     ApoI
     AscI     AseI    AsiSI    BamHI     BbsI     BcgI     BclI    BfrBI
     BlpI     BmtI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI
     EagI     EarI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PmeI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SgfI    SgrAI
    SnaBI     SpeI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI
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