Restriction map for pF1KB8598
 (Linear) (Six-base) MAPSORT of: KIBB8598.seq  Check: 2442  from: 1  to: 2328



KIBB8598.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      78    2328 
   Size:         78    2250
 
AccI GT'mk_AC

Cuts at:      0    1810    2328 
   Size:       1810     518
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55    1495    1819    2328 
   Size:         55    1440     324     509

  Fragments arranged by size: 

                1440     509     324      55
 
AflII C'TTAA_G

Cuts at:      0     274     310    2328 
   Size:        274      36    2018

  Fragments arranged by size: 

                2018     274      36
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1630    2328 
   Size:       1630     698
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     182     214    2328 
   Size:        182      32    2114

  Fragments arranged by size: 

                2114     182      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     772    1872    2328 
   Size:        772    1100     456

  Fragments arranged by size: 

                1100     772     456
 
ApaI G_GGCC'C

Cuts at:      0     667    2328 
   Size:        667    1661
 
ApoI r'AATT_y

Cuts at:      0     280     349     404     748     902     916     924    2328 
   Size:        280      69      55     344     154      14       8    1404

  Fragments arranged by size: 

                1404     344     280     154      69      55      14       8
            
 
AvaI C'yCGr_G

Cuts at:      0    2231    2328 
   Size:       2231      97
 
AvrII C'CTAG_G

Cuts at:      0     996    2328 
   Size:        996    1332
 
BanII G_rGCy'C

Cuts at:      0     134     243     667    1624    2328 
   Size:        134     109     424     957     704

  Fragments arranged by size: 

                 957     704     424     134     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1334    1347    1376    2120    2328 
   Size:       1334      13      29     744     208

  Fragments arranged by size: 

                1334     744     208      29      13
 
BclI T'GATC_A

Cuts at:      0    2243    2328 
   Size:       2243      85
 
BfrBI ATG'CAT

Cuts at:      0     652    2255    2328 
   Size:        652    1603      73

  Fragments arranged by size: 

                1603     652      73
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1724    2328 
   Size:       1724     604
 
BglII A'GATC_T

Cuts at:      0      56    1545    2328 
   Size:         56    1489     783

  Fragments arranged by size: 

                1489     783      56
 
BlpI GC'TnA_GC

Cuts at:      0    1618    2328 
   Size:       1618     710
 
Bme1580I G_kGCm'C

Cuts at:      0     667    2328 
   Size:        667    1661
 
BmgBI CAC'GTC

Cuts at:      0    1739    2328 
   Size:       1739     589
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     760    2328 
   Size:        760    1568
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1518    2328 
   Size:       1518     810
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215     254    2202    2328 
   Size:        215      39    1948     126

  Fragments arranged by size: 

                1948     215     126      39
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    2328 
   Size:        826    1502
 
BsaAI yAC'GTr

Cuts at:      0     624    2328 
   Size:        624    1704
 
BsaBI GATnn'nnATC

Cuts at:      0    1825    2328 
   Size:       1825     503
 
BsaHI Gr'CG_yC

Cuts at:      0    1807    2328 
   Size:       1807     521
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     182     212     492     522    1264    1294    1754    1784 
   Size:        182      30     280      30     742      30     460      30

Cuts at:   1784    2328 
   Size:        544

  Fragments arranged by size: 

                 742     544     460     280     182      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     203     251    1327    2328 
   Size:        203      48    1076    1001

  Fragments arranged by size: 

                1076    1001     203      48
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1885    2328 
   Size:       1885     443
 
BsiHKAI G_wGCw'C

Cuts at:      0     134     243    1624    2328 
   Size:        134     109    1381     704

  Fragments arranged by size: 

                1381     704     134     109
 
BsmI GAATG_Cn'

Cuts at:      0    1150    1607    2328 
   Size:       1150     457     721

  Fragments arranged by size: 

                1150     721     457
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     243     667    1624    2328 
   Size:        134     109     424     957     704

  Fragments arranged by size: 

                 957     704     424     134     109
 
BspHI T'CATG_A

Cuts at:      0     837    2153    2328 
   Size:        837    1316     175

  Fragments arranged by size: 

                1316     837     175
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      78    2328 
   Size:         78    2250
 
BsrFI r'CCGG_y

Cuts at:      0      76    2328 
   Size:         76    2252
 
BsrGI T'GTAC_A

Cuts at:      0      92     612    1098    1651    2328 
   Size:         92     520     486     553     677

  Fragments arranged by size: 

                 677     553     520     486      92
 
BssSI C'ACGA_G

Cuts at:      0     185    2328 
   Size:        185    2143
 
BstEII G'GTnAC_C

Cuts at:      0    1221    2328 
   Size:       1221    1107
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     126    2328 
   Size:        126    2202
 
BstYI r'GATC_y

Cuts at:      0      56    1545    2328 
   Size:         56    1489     783

  Fragments arranged by size: 

                1489     783      56
 
BtgI C'CryG_G

Cuts at:      0      27    2328 
   Size:         27    2301
 
ClaI AT'CG_AT

Cuts at:      0     378    2328 
   Size:        378    1950
 
DraI TTT'AAA

Cuts at:      0     551    1079    2328 
   Size:        551     528    1249

  Fragments arranged by size: 

                1249     551     528
 
EaeI y'GGCC_r

Cuts at:      0     110    1725    2138    2328 
   Size:        110    1615     413     190

  Fragments arranged by size: 

                1615     413     190     110
 
EarI CTCTTCn'nnn_

Cuts at:      0     813    2328 
   Size:        813    1515
 
EcoICRI GAG'CTC

Cuts at:      0     132     241    1622    2328 
   Size:        132     109    1381     706

  Fragments arranged by size: 

                1381     706     132     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     215     254    1495    1819    2202    2328 
   Size:         55     160      39    1241     324     383     126

  Fragments arranged by size: 

                1241     383     324     160     126      55      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     663     664    1628    2199    2328 
   Size:        663       1     964     571     129

  Fragments arranged by size: 

                 964     663     571     129       1
 
EcoRI G'AATT_C

Cuts at:      0     748    2328 
   Size:        748    1580
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     312    2328 
   Size:        312    2016
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      65      97     492     524    1530    1562    2328 
   Size:         65      32     395      32    1006      32     766

  Fragments arranged by size: 

                1006     766     395      65      32      32      32
 
HincII GTy'rAC

Cuts at:      0       8     674    1811    2046    2328 
   Size:          8     666    1137     235     282

  Fragments arranged by size: 

                1137     666     282     235       8
 
HindIII A'AGCT_T

Cuts at:      0    1481    2328 
   Size:       1481     847
 
HpaI GTT'AAC

Cuts at:      0     674    2046    2328 
   Size:        674    1372     282

  Fragments arranged by size: 

                1372     674     282
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     998    1655    2328 
   Size:        998     657     673

  Fragments arranged by size: 

                 998     673     657
 
MscI TGG'CCA

Cuts at:      0     112    2328 
   Size:        112    2216
 
MslI CAynn'nnrTG

Cuts at:      0    1534    1612    2053    2158    2328 
   Size:       1534      78     441     105     170

  Fragments arranged by size: 

                1534     441     170     105      78
 
MspA1I CmG'CkG

Cuts at:      0    1872    2211    2328 
   Size:       1872     339     117

  Fragments arranged by size: 

                1872     339     117
 
NaeI GCC'GGC

Cuts at:      0      78    2328 
   Size:         78    2250
 
NgoMIV G'CCGG_C

Cuts at:      0      76    2328 
   Size:         76    2252
 
NsiI A_TGCA'T

Cuts at:      0     654    2257    2328 
   Size:        654    1603      71

  Fragments arranged by size: 

                1603     654      71
 
NspI r_CATG'y

Cuts at:      0     966    1521    2328 
   Size:        966     555     807

  Fragments arranged by size: 

                 966     807     555
 
PfoI T'CCnGG_A

Cuts at:      0     986    2328 
   Size:        986    1342
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     182     214     866     898    2328 
   Size:        182      32     652      32    1430

  Fragments arranged by size: 

                1430     652     182      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1628    2199    2328 
   Size:       1628     571     129

  Fragments arranged by size: 

                1628     571     129
 
PsiI TTA'TAA

Cuts at:      0     149    2024    2328 
   Size:        149    1875     304

  Fragments arranged by size: 

                1875     304     149
 
PspOMI G'GGCC_C

Cuts at:      0     663    2328 
   Size:        663    1665
 
PspXI vC'TCGA_Gb

Cuts at:      0    2231    2328 
   Size:       2231      97
 
PvuII CAG'CTG

Cuts at:      0    1872    2211    2328 
   Size:       1872     339     117

  Fragments arranged by size: 

                1872     339     117
 
SacI G_AGCT'C

Cuts at:      0     134     243    1624    2328 
   Size:        134     109    1381     704

  Fragments arranged by size: 

                1381     704     134     109
 
SalI G'TCGA_C

Cuts at:      0    1809    2328 
   Size:       1809     519
 
ScaI AGT'ACT

Cuts at:      0     744    2328 
   Size:        744    1584
 
SfcI C'TryA_G

Cuts at:      0    2313    2328 
   Size:       2313      15
 
SmlI C'TyrA_G

Cuts at:      0     274     310     805    2231    2328 
   Size:        274      36     495    1426      97

  Fragments arranged by size: 

                1426     495     274      97      36
 
SpeI A'CTAG_T

Cuts at:      0     160    2328 
   Size:        160    2168
 
SspI AAT'ATT

Cuts at:      0    2281    2328 
   Size:       2281      47
 
StyI C'CwwG_G

Cuts at:      0     996    2328 
   Size:        996    1332
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1891    2328 
   Size:       1891     437
 
TatI w'GTAC_w

Cuts at:      0      92     157     342     612     742    1098    1361    1496 
   Size:         92      65     185     270     130     356     263     135

Cuts at:   1496    1651    1838    2328 
   Size:        155     187     490

  Fragments arranged by size: 

                 490     356     270     263     187     185     155     135
                 130      92      65
 
XbaI T'CTAG_A

Cuts at:      0     979    2328 
   Size:        979    1349
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2188    2328 
   Size:       2188     140
 
XhoI C'TCGA_G

Cuts at:      0    2231    2328 
   Size:       2231      97
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII     AlfI     AloI    AlwNI     ApaI
     ApoI     AvaI    AvrII    BanII     BbsI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BspMI    BsrFI    BsrGI    BssSI   BstEII    BstXI
    BstYI     BtgI     ClaI     DraI     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     HpaI     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NsiI     NspI     PfoI
     PpiI    PpuMI     PsiI   PspOMI    PspXI    PvuII     SacI     SalI
     ScaI     SfcI     SmlI     SpeI     SspI     StyI    TaqII     TatI
     XbaI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI     AleI
    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BmtI   Bpu10I     BsaI    BsaWI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrDI   BssHII   BstAPI
    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI    CspCI   DraIII     DrdI
     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI     NheI
     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI     PmlI
    PshAI     PsrI     PstI     PvuI    RsrII    SacII    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XmaI     XmnI
     ZraI
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