Restriction map for pF1KB6452
 (Linear) (Six-base) MAPSORT of: KIBB6452.seq  Check: 3704  from: 1  to: 1050



KIBB6452.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AccI GT'mk_AC

Cuts at:      0      35    1050 
   Size:         35    1015
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     796    1050 
   Size:        232     564     254

  Fragments arranged by size: 

                 564     254     232
 
AflIII A'CryG_T

Cuts at:      0     644    1050 
   Size:        644     406
 
AhdI GACnn_n'nnGTC

Cuts at:      0     315     877    1050 
   Size:        315     562     173

  Fragments arranged by size: 

                 562     315     173
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     683     715    1050 
   Size:        683      32     335

  Fragments arranged by size: 

                 683     335      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     384     939    1050 
   Size:        384     555     111

  Fragments arranged by size: 

                 555     384     111
 
ApaI G_GGCC'C

Cuts at:      0      30    1050 
   Size:         30    1020
 
ApoI r'AATT_y

Cuts at:      0     541    1050 
   Size:        541     509
 
AseI AT'TA_AT

Cuts at:      0     173    1050 
   Size:        173     877
 
AvaI C'yCGr_G

Cuts at:      0      29      93    1050 
   Size:         29      64     957

  Fragments arranged by size: 

                 957      64      29
 
BanII G_rGCy'C

Cuts at:      0      30     608    1050 
   Size:         30     578     442

  Fragments arranged by size: 

                 578     442      30
 
BglI GCCn_nnn'nGGC

Cuts at:      0     952    1050 
   Size:        952      98
 
BglII A'GATC_T

Cuts at:      0     897    1050 
   Size:        897     153
 
Bme1580I G_kGCm'C

Cuts at:      0      30    1050 
   Size:         30    1020
 
BmgBI CAC'GTC

Cuts at:      0      90    1050 
   Size:         90     960
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      81    1050 
   Size:         81     969
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277    1050 
   Size:        277     773
 
Bpu10I CC'TnA_GC

Cuts at:      0     557    1050 
   Size:        557     493
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     567    1050 
   Size:        567     483
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1039    1050 
   Size:       1039      11
 
BsaWI w'CCGG_w

Cuts at:      0     401    1050 
   Size:        401     649
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     590     620    1050 
   Size:        590      30     430

  Fragments arranged by size: 

                 590     430      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      72     598    1050 
   Size:         72     526     452

  Fragments arranged by size: 

                 526     452      72
 
BseYI C'CCAG_C

Cuts at:      0      98     842     998    1050 
   Size:         98     744     156      52

  Fragments arranged by size: 

                 744     156      98      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1028    1050 
   Size:       1028      22
 
BsiEI CG_ry'CG

Cuts at:      0      14    1050 
   Size:         14    1036
 
BsiHKAI G_wGCw'C

Cuts at:      0     608     965    1050 
   Size:        608     357      85

  Fragments arranged by size: 

                 608     357      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      30     608     965    1050 
   Size:         30     578     357      85

  Fragments arranged by size: 

                 578     357      85      30
 
BspHI T'CATG_A

Cuts at:      0     488    1050 
   Size:        488     562
 
BsrDI GCAATG_nn'

Cuts at:      0     734    1050 
   Size:        734     316
 
BssSI C'ACGA_G

Cuts at:      0     610    1050 
   Size:        610     440
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1015    1050 
   Size:       1015      35
 
BstBI TT'CG_AA

Cuts at:      0     129     684    1050 
   Size:        129     555     366

  Fragments arranged by size: 

                 555     366     129
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     747    1050 
   Size:        747     303
 
BstYI r'GATC_y

Cuts at:      0     380     798     897    1050 
   Size:        380     418      99     153

  Fragments arranged by size: 

                 418     380     153      99
 
Bsu36I CC'TnA_GG

Cuts at:      0     887    1050 
   Size:        887     163
 
BtgI C'CryG_G

Cuts at:      0      14     702     956    1050 
   Size:         14     688     254      94

  Fragments arranged by size: 

                 688     254      94      14
 
EaeI y'GGCC_r

Cuts at:      0      11    1050 
   Size:         11    1039
 
EagI C'GGCC_G

Cuts at:      0      11    1050 
   Size:         11    1039
 
EcoICRI GAG'CTC

Cuts at:      0     606    1050 
   Size:        606     444
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232     277     796    1050 
   Size:        232      45     519     254

  Fragments arranged by size: 

                 519     254     232      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0      26     309     891    1050 
   Size:         26     283     582     159

  Fragments arranged by size: 

                 582     283     159      26
 
EcoRI G'AATT_C

Cuts at:      0     541    1050 
   Size:        541     509
 
EcoRV GAT'ATC

Cuts at:      0     399     816    1050 
   Size:        399     417     234

  Fragments arranged by size: 

                 417     399     234
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     301     333     590     622     863     895    1050 
   Size:        301      32     257      32     241      32     155

  Fragments arranged by size: 

                 301     257     241     155      32      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     467    1050 
   Size:        467     583
 
MspA1I CmG'CkG

Cuts at:      0      16    1050 
   Size:         16    1034
 
NcoI C'CATG_G

Cuts at:      0     702     956    1050 
   Size:        702     254      94

  Fragments arranged by size: 

                 702     254      94
 
NspI r_CATG'y

Cuts at:      0     648    1050 
   Size:        648     402
 
PciI A'CATG_T

Cuts at:      0     644    1050 
   Size:        644     406
 
PpuMI rG'GwC_Cy

Cuts at:      0     309    1050 
   Size:        309     741
 
PspOMI G'GGCC_C

Cuts at:      0      26    1050 
   Size:         26    1024
 
SacI G_AGCT'C

Cuts at:      0     608    1050 
   Size:        608     442
 
SacII CC_GC'GG

Cuts at:      0      17    1050 
   Size:         17    1033
 
ScaI AGT'ACT

Cuts at:      0      70    1050 
   Size:         70     980
 
SmaI CCC'GGG

Cuts at:      0      31    1050 
   Size:         31    1019
 
SmlI C'TyrA_G

Cuts at:      0     582    1050 
   Size:        582     468
 
StyI C'CwwG_G

Cuts at:      0     702     740     956    1050 
   Size:        702      38     216      94

  Fragments arranged by size: 

                 702     216      94      38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     980    1050 
   Size:        980      70
 
TatI w'GTAC_w

Cuts at:      0      68     563    1050 
   Size:         68     495     487

  Fragments arranged by size: 

                 495     487      68
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     954    1050 
   Size:        954      96
 
XmaI C'CCGG_G

Cuts at:      0      29    1050 
   Size:         29    1021
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AloI    AlwNI     ApaI     ApoI
     AseI     AvaI    BanII     BglI    BglII Bme1580I    BmgBI     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BsrDI    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI     EaeI     EagI  EcoICRI
  Eco57MI EcoO109I    EcoRI    EcoRV    Hin4I     MslI   MspA1I     NcoI
     NspI     PciI    PpuMI   PspOMI     SacI    SacII     ScaI     SmaI
     SmlI     StyI    TaqII     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI    ApaLI     AscI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI
     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstEII  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EarI
     EciI    EcoNI     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmnI     ZraI
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