Restriction map for pF1KB8644
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrDI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstF5I      BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraIII      
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HhaI        HinP1I      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        MvnI        NaeI        
NarI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SapI        
Sau3AI      SbfI        ScaI        SexAI       SfaNI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tru9I       Tsp509I     XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII          15  GACGTC               GACGT^C
AccI           12  GTCGAC               GT^[AC][GT]AC
AflIII        288  ACATGT               A^C[GA][CT]GT
AlwNI          96  CAGGGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         397  GTGCTC               G[AT]GC[AT]^C
AspI          432  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          441  TGGCCA               TGG^CCA
BanII         453  GGGCTC               G[GA]GC[CT]^C
BbvCI         188  CCTCAGC              CCTCAGC(-5/-2)
BbvI           92  GCAGC                GCAGC(8/12)
BsaAI         313  TACGTG               [CT]AC^GT[GA]
BsiHKAI       397  GTGCTC               G[AT]GC[AT]^C
BsmAI         430  GAGAC                GTCTC(1/5)
BsmBI         430  GAGACG               CGTCTC(1/5)
BspLU11I      288  ACATGT               A^CATGT
BssSI         427  CACGAG               CACGAG(-5/-1)
BstEII        336  GGTGACC              G^GT[ACGT]ACC
BstPI         336  GGTGACC              G^GT[ACGT]ACC
DrdI            3  GACTTCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          441  TGGCCA               [CT]^GGCC[GA]
EcoNI          77  CCTTCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       336  GGTGACC              G^GT[ACGT]ACC
EcoT14I       243  CCATGG               C^C[AT][AT]GG
Fnu4HI         92  GCAGC                GC^[ACGT]GC
HapII         183  CCGG                 C^CGG
HgaI          212  GCGTC                GACGC(5/10)
HincII         12  GTCGAC               GT[CT]^[GA]AC
HindII         12  GTCGAC               GT[CT]^[GA]AC
HinfI          10  GAGTC                G^A[ACGT]TC
HpaII         183  CCGG                 C^CGG
ItaI           92  GCAGC                GC^[ACGT]GC
MboII         281  TCTTC                GAAGA(8/7)
MluNI         441  TGGCCA               TGG^CCA
MscI          441  TGGCCA               TGG^CCA
MspI          183  CCGG                 C^CGG
NcoI          243  CCATGG               C^CATGG
NspI          288  ACATGT               [GA]CATG^[CT]
PflFI         432  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           10  GAGTC                GAGTC(4/5)
SalI           12  GTCGAC               G^TCGAC
SmaI          182  CCCGGG               CCC^GGG
StyI          243  CCATGG               C^C[AT][AT]GG
TseI           92  GCAGC                G^C[AT]GC
TspRI         320  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       432  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         182  CCCGGG               C^CCGGG
XmaI          182  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI           15  GACGTC               G[GA]^CG[CT]C
              211  GGCGTC              
AfaI          286  GTAC                 GT^AC
              312  GTAC                
AluI          192  AGCT                 AG^CT
              407  AGCT                
BcnI          182  CCCGG                CC^[GC]GG
              183  CCGGG               
BsaHI          15  GACGTC               G[GA]^CG[CT]C
              211  GGCGTC              
BsrBI          37  CCGCTC               CCGCTC(-3/-3)
              106  GAGCGG              
Cac8I         193  GCTGGC               GC[ACGT]^[ACGT]GC
              408  GCTGGC              
DdeI          189  CTCAG                C^T[ACGT]AG
              404  CTCAG               
DraII         128  GGGACCT              [GA]G^G[ACGT]CC[CT]
              163  AGGACCT             
DsaI          243  CCATGG               C^C[GA][CT]GG
              386  CCGTGG              
EcoO109I      128  GGGACCT              [GA]G^G[ACGT]CC[CT]
              163  AGGACCT             
FauI           36  CCCGC                CCCGC(4/6)
              108  GCGGG               
Hin1I          15  GACGTC               G[GA]^CG[CT]C
              211  GGCGTC              
MaeIII         18  GTCAC                ^GT[ACGT]AC
              337  GTGAC               
MspA1I        191  CAGCTG               C[AC]G^C[GT]G
              406  CAGCTG              
NciI          182  CCCGG                CC^[GC]GG
              183  CCGGG               
NlaIII        244  CATG                 CATG^
              289  CATG                
PflMI         381  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              444  CCAGGTCGTGG         
PpuMI         128  GGGACCT              [GA]G^G[AT]CC[CT]
              163  AGGACCT             
PvuII         191  CAGCTG               CAG^CTG
              406  CAGCTG              
RsaI          286  GTAC                 GT^AC
              312  GTAC                
TaqI           13  TCGA                 T^CGA
              302  TCGA                
Tsp45I         18  GTCAC                ^GT[GC]AC
              337  GTGAC               
TthHB8I        13  TCGA                 T^CGA
              302  TCGA                
Van91I        381  CCAGGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              444  CCAGGTCGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AvaI        AvaII       BmyI        BsaJI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BsrI        BssKI       BstNI       Cfr13I      EcoRII      
HaeIII      HphI        MaeII       MnlI        MvaI        
MwoI        NlaIV       Sau96I      ScrFI       
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