Restriction map for
pF1KB8644
(Circular) (Six-base) MAPSORT of: pF1KB8644.seq Check: 1601 from: 1 to: 3572
pF1KB8644 3572 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AatII G_ACGT'C
Cuts at: 127 127
Size: 3572
AccI GT'mk_AC
Cuts at: 121 610 121
Size: 489 3083
Acc65I G'GTAC_C
Cuts at: 588 588
Size: 3572
AclI AA'CG_TT
Cuts at: 3412 3412
Size: 3572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 1397 1829 2576 2844 98
Size: 1299 432 747 268 826
Fragments arranged by size:
1299 826 747 432 268
AflIII A'CryG_T
Cuts at: 396 924 2028 3005 396
Size: 528 1104 977 963
Fragments arranged by size:
1104 977 963 528
AgeI A'CCGG_T
Cuts at: 1960 1960
Size: 3572
AlwNI CAG_nnn'CTG
Cuts at: 209 877 2444 209
Size: 668 1567 1337
Fragments arranged by size:
1567 1337 668
ApaLI G'TGCA_C
Cuts at: 2342 2342
Size: 3572
ApoI r'AATT_y
Cuts at: 576 2925 3181 576
Size: 2349 256 967
Fragments arranged by size:
2349 967 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3572
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
AvaI C'yCGr_G
Cuts at: 233 290 367 592 233
Size: 57 77 225 3213
Fragments arranged by size:
3213 225 77 57
BamHI G'GATC_C
Cuts at: 597 597
Size: 3572
BanI G'GyrC_C
Cuts at: 588 1002 1250 1285 588
Size: 414 248 35 2875
Fragments arranged by size:
2875 414 248 35
BanII G_rGCy'C
Cuts at: 565 586 1616 565
Size: 21 1030 2521
Fragments arranged by size:
2521 1030 21
BbeI G_GCGC'C
Cuts at: 1006 1254 1006
Size: 248 3324
BbvCI CC'TCA_GC
Cuts at: 297 297
Size: 3572
BciVI GTATCCnnnnn_n'
Cuts at: 1470 2231 3572 1470
Size: 761 1341 1470
Fragments arranged by size:
1470 1341 761
BclI T'GATC_A
Cuts at: 1092 1092
Size: 3572
BglII A'GATC_T
Cuts at: 1087 3124 1087
Size: 2037 1535
BlpI GC'TnA_GC
Cuts at: 684 684
Size: 3572
Bme1580I G_kGCm'C
Cuts at: 143 463 1197 1290 2346 143
Size: 320 734 93 1056 1369
Fragments arranged by size:
1369 1056 734 320 93
BmgBI CAC'GTC
Cuts at: 542 542
Size: 3572
BmrI ACTGGGnnnn_n'
Cuts at: 127 160 784 865 1200 3344 127
Size: 33 624 81 335 2144 355
Fragments arranged by size:
2144 624 355 335 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1939
Size: 3572
Bpu10I CC'TnA_GC
Cuts at: 297 297
Size: 3572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 1627 2119 2417 748
Size: 879 492 298 1903
Fragments arranged by size:
1903 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3276 3276
Size: 3572
BsaAI yAC'GTr
Cuts at: 423 925 423
Size: 502 3070
BsaBI GATnn'nnATC
Cuts at: 6 1086 3123 6
Size: 1080 2037 455
Fragments arranged by size:
2037 1080 455
BsaHI Gr'CG_yC
Cuts at: 124 320 1003 1251 1953 3449 124
Size: 196 683 248 702 1496 247
Fragments arranged by size:
1496 702 683 248 247 196
BsaWI w'CCGG_w
Cuts at: 761 1282 1960 2234 2381 2792 761
Size: 521 678 274 147 411 1541
Fragments arranged by size:
1541 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 244 997 1755 2332 2828 244
Size: 753 758 577 496 988
Fragments arranged by size:
988 758 753 577 496
BsiEI CG_ry'CG
Cuts at: 104 1114 1160 2368 104
Size: 1010 46 1208 1308
Fragments arranged by size:
1308 1208 1010 46
BsiHKAI G_wGCw'C
Cuts at: 509 586 1364 1554 2346 509
Size: 77 778 190 792 1735
Fragments arranged by size:
1735 792 778 190 77
BsmBI CGTCTCn'nnnn_
Cuts at: 532 532
Size: 3572
Bsp1286I G_dGCh'C
Cuts at: 143 463 509 565 586 1197 1290 1364 1554
Size: 320 46 56 21 611 93 74 190
Cuts at: 1554 1616 2346 143
Size: 62 730 1369
Fragments arranged by size:
1369 730 611 320 190 93 74 62
56 46 21
BspEI T'CCGG_A
Cuts at: 2792 2792
Size: 3572
BspHI T'CATG_A
Cuts at: 2748 2748
Size: 3572
BspMI ACCTGCnnnn'nnnn_
Cuts at: 622 836 1138 1519 622
Size: 214 302 381 2675
Fragments arranged by size:
2675 381 302 214
BsrBI CCG'CTC
Cuts at: 50 147 216 1864 1918 3402 3567 50
Size: 97 69 1648 54 1484 165 55
Fragments arranged by size:
1648 1484 165 97 69 55 54
BsrDI GCAATG_nn'
Cuts at: 1484 1484
Size: 3572
BsrFI r'CCGG_y
Cuts at: 1570 1751 1960 1570
Size: 181 209 3182
Fragments arranged by size:
3182 209 181
BssSI C'ACGA_G
Cuts at: 535 1843 2201 535
Size: 1308 358 1906
Fragments arranged by size:
1906 1308 358
BstAPI GCAn_nnn'nTGC
Cuts at: 949 949
Size: 3572
BstBI TT'CG_AA
Cuts at: 1933 1933
Size: 3572
BstEII G'GTnAC_C
Cuts at: 444 444
Size: 3572
BstYI r'GATC_y
Cuts at: 1 597 1064 1087 1422 1668 2680 2766 2778
Size: 596 467 23 335 246 1012 86 12
Cuts at: 2778 3124 1
Size: 346 449
Fragments arranged by size:
1012 596 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 351 494 351
Size: 143 3429
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1522 1703 1866 1522
Size: 181 163 3228
Fragments arranged by size:
3228 181 163
BtsI GCAGTG_nn'
Cuts at: 815 815
Size: 3572
DraI TTT'AAA
Cuts at: 571 571
Size: 3572
DrdI GACnn_nn'nnGTC
Cuts at: 117 1278 2136 117
Size: 1161 858 1553
Fragments arranged by size:
1553 1161 858
EaeI y'GGCC_r
Cuts at: 549 1157 1331 1722 1749 3094 549
Size: 608 174 391 27 1345 1027
Fragments arranged by size:
1345 1027 608 391 174 27
EagI C'GGCC_G
Cuts at: 1157 1157
Size: 3572
EarI CTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1780 2090 2236 3382 1780
Size: 310 146 1146 1970
Fragments arranged by size:
1970 1146 310 146
EcoICRI GAG'CTC
Cuts at: 584 584
Size: 3572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 1397 1829 2576 2844 98
Size: 1299 432 747 268 826
Fragments arranged by size:
1299 826 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 189 189
Size: 3572
EcoO109I rG'GnC_Cy
Cuts at: 237 272 711 237
Size: 35 439 3098
Fragments arranged by size:
3098 439 35
EcoRI G'AATT_C
Cuts at: 576 576
Size: 3572
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1591 1591
Size: 3572
FspI TGC'GCA
Cuts at: 1353 1353
Size: 3572
HaeII r_GCGC'y
Cuts at: 1006 1254 2276 3255 1006
Size: 248 1022 979 1323
Fragments arranged by size:
1323 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 361 393 529 561 1414 1444 1446
Size: 32 220 32 136 32 853 30 2
Cuts at: 1446 1476 109
Size: 30 2205
Fragments arranged by size:
2205 853 220 136 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 122 611 122
Size: 489 3083
KasI G'GCGC_C
Cuts at: 1002 1250 1002
Size: 248 3324
KpnI G_GTAC'C
Cuts at: 592 592
Size: 3572
MluI A'CGCG_T
Cuts at: 3005 3005
Size: 3572
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2243 2427 2243
Size: 184 3388
MscI TGG'CCA
Cuts at: 551 1333 551
Size: 782 2790
MslI CAynn'nnrTG
Cuts at: 1561 1688 1561
Size: 127 3445
MspA1I CmG'CkG
Cuts at: 301 516 683 997 1357 2370 2615 301
Size: 215 167 314 360 1013 245 1258
Fragments arranged by size:
1258 1013 360 314 245 215 167
NaeI GCC'GGC
Cuts at: 1753 1753
Size: 3572
NarI GG'CG_CC
Cuts at: 1003 1251 1003
Size: 248 3324
NcoI C'CATG_G
Cuts at: 351 351
Size: 3572
NgoMIV G'CCGG_C
Cuts at: 1751 1751
Size: 3572
NspI r_CATG'y
Cuts at: 400 625 1125 2032 400
Size: 225 500 907 1940
Fragments arranged by size:
1940 907 500 225
PciI A'CATG_T
Cuts at: 396 2028 396
Size: 1632 1940
PflMI CCAn_nnn'nTGG
Cuts at: 495 558 495
Size: 63 3509
PmeI GTTT'AAAC
Cuts at: 571 571
Size: 3572
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 2743 2711
Size: 32 3540
PpuMI rG'GwC_Cy
Cuts at: 237 272 237
Size: 35 3537
PstI C_TGCA'G
Cuts at: 619 1304 619
Size: 685 2887
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3572
PvuII CAG'CTG
Cuts at: 301 516 997 1357 301
Size: 215 481 360 2516
Fragments arranged by size:
2516 481 360 215
RsrII CG'GwC_CG
Cuts at: 1767 1767
Size: 3572
SacI G_AGCT'C
Cuts at: 586 586
Size: 3572
SalI G'TCGA_C
Cuts at: 120 609 120
Size: 489 3083
SapI GCTCTTCn'nnn_
Cuts at: 1595 1805 1595
Size: 210 3362
SbfI CC_TGCA'GG
Cuts at: 619 619
Size: 3572
ScaI AGT'ACT
Cuts at: 3089 3089
Size: 3572
SfcI C'TryA_G
Cuts at: 33 615 1300 2293 2484 33
Size: 582 685 993 191 1121
Fragments arranged by size:
1121 993 685 582 191
SfoI GGC'GCC
Cuts at: 1004 1252 1004
Size: 248 3324
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3572
SmaI CCC'GGG
Cuts at: 292 594 292
Size: 302 3270
SmlI C'TyrA_G
Cuts at: 727 1642 2134 2396 727
Size: 915 492 262 1903
Fragments arranged by size:
1903 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3572
SphI G_CATG'C
Cuts at: 625 1125 625
Size: 500 3072
SrfI GCCC'GGGC
Cuts at: 292 292
Size: 3572
StyI C'CwwG_G
Cuts at: 351 706 1060 351
Size: 355 354 2863
Fragments arranged by size:
2863 355 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1155 1288 1747 1956 1155
Size: 133 459 209 2771
Fragments arranged by size:
2771 459 209 133
TatI w'GTAC_w
Cuts at: 3087 3087
Size: 3572
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3089 3121 3089
Size: 32 3540
Tth111I GACn'n_nGTC
Cuts at: 543 1369 543
Size: 826 2746
XbaI T'CTAG_A
Cuts at: 603 603
Size: 3572
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1067 1067
Size: 3572
XmaI C'CCGG_G
Cuts at: 290 592 290
Size: 302 3270
ZraI GAC'GTC
Cuts at: 125 125
Size: 3572
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmI BsrGI BssHII
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SspI StuI SwaI XhoI XmnI