Restriction map for pF1KB8644
 (Linear) (Six-base) MAPSORT of: KIBB8644.seq  Check: 6356  from: 1  to: 459



KIBB8644.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AatII G_ACGT'C

Cuts at:      0      19     459 
   Size:         19     440
 
AccI GT'mk_AC

Cuts at:      0      13     459 
   Size:         13     446
 
AflIII A'CryG_T

Cuts at:      0     288     459 
   Size:        288     171
 
AlwNI CAG_nnn'CTG

Cuts at:      0     101     459 
   Size:        101     358
 
AvaI C'yCGr_G

Cuts at:      0     125     182     259     459 
   Size:        125      57      77     200

  Fragments arranged by size: 

                 200     125      77      57
 
BanII G_rGCy'C

Cuts at:      0     457     459 
   Size:        457       2
 
BbvCI CC'TCA_GC

Cuts at:      0     189     459 
   Size:        189     270
 
Bme1580I G_kGCm'C

Cuts at:      0      35     355     459 
   Size:         35     320     104

  Fragments arranged by size: 

                 320     104      35
 
BmgBI CAC'GTC

Cuts at:      0     434     459 
   Size:        434      25
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      19      52     459 
   Size:         19      33     407

  Fragments arranged by size: 

                 407      33      19
 
Bpu10I CC'TnA_GC

Cuts at:      0     189     459 
   Size:        189     270
 
BsaAI yAC'GTr

Cuts at:      0     315     459 
   Size:        315     144
 
BsaHI Gr'CG_yC

Cuts at:      0      16     212     459 
   Size:         16     196     247

  Fragments arranged by size: 

                 247     196      16
 
BseYI C'CCAG_C

Cuts at:      0     136     459 
   Size:        136     323
 
BsiHKAI G_wGCw'C

Cuts at:      0     401     459 
   Size:        401      58
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     424     459 
   Size:        424      35
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     355     401     457     459 
   Size:         35     320      46      56       2

  Fragments arranged by size: 

                 320      56      46      35       2
 
BsrBI CCG'CTC

Cuts at:      0      39     108     459 
   Size:         39      69     351

  Fragments arranged by size: 

                 351      69      39
 
BssSI C'ACGA_G

Cuts at:      0     427     459 
   Size:        427      32
 
BstEII G'GTnAC_C

Cuts at:      0     336     459 
   Size:        336     123
 
BtgI C'CryG_G

Cuts at:      0     243     386     459 
   Size:        243     143      73

  Fragments arranged by size: 

                 243     143      73
 
DrdI GACnn_nn'nnGTC

Cuts at:      0       9     459 
   Size:          9     450
 
EaeI y'GGCC_r

Cuts at:      0     441     459 
   Size:        441      18
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      81     459 
   Size:         81     378
 
EcoO109I rG'GnC_Cy

Cuts at:      0     129     164     459 
   Size:        129      35     295

  Fragments arranged by size: 

                 295     129      35
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       1      33     253     285     421     453     459 
   Size:          1      32     220      32     136      32       6

  Fragments arranged by size: 

                 220     136      32      32      32       6       1
 
HincII GTy'rAC

Cuts at:      0      14     459 
   Size:         14     445
 
MscI TGG'CCA

Cuts at:      0     443     459 
   Size:        443      16
 
MspA1I CmG'CkG

Cuts at:      0     193     408     459 
   Size:        193     215      51

  Fragments arranged by size: 

                 215     193      51
 
NcoI C'CATG_G

Cuts at:      0     243     459 
   Size:        243     216
 
NspI r_CATG'y

Cuts at:      0     292     459 
   Size:        292     167
 
PciI A'CATG_T

Cuts at:      0     288     459 
   Size:        288     171
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     387     450     459 
   Size:        387      63       9

  Fragments arranged by size: 

                 387      63       9
 
PpuMI rG'GwC_Cy

Cuts at:      0     129     164     459 
   Size:        129      35     295

  Fragments arranged by size: 

                 295     129      35
 
PvuII CAG'CTG

Cuts at:      0     193     408     459 
   Size:        193     215      51

  Fragments arranged by size: 

                 215     193      51
 
SalI G'TCGA_C

Cuts at:      0      12     459 
   Size:         12     447
 
SmaI CCC'GGG

Cuts at:      0     184     459 
   Size:        184     275
 
SrfI GCCC'GGGC

Cuts at:      0     184     459 
   Size:        184     275
 
StyI C'CwwG_G

Cuts at:      0     243     459 
   Size:        243     216
 
Tth111I GACn'n_nGTC

Cuts at:      0     435     459 
   Size:        435      24
 
XmaI C'CCGG_G

Cuts at:      0     182     459 
   Size:        182     277
 
ZraI GAC'GTC

Cuts at:      0      17     459 
   Size:         17     442
 

 Enzymes that do cut:

    AatII     AccI   AflIII    AlwNI     AvaI    BanII    BbvCI Bme1580I
    BmgBI     BmrI   Bpu10I    BsaAI    BsaHI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BsrBI    BssSI   BstEII     BtgI     DrdI     EaeI    EcoNI
 EcoO109I    Hin4I   HincII     MscI   MspA1I     NcoI     NspI     PciI
    PflMI    PpuMI    PvuII     SalI     SmaI     SrfI     StyI  Tth111I
     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     SwaI    TaqII     TatI     TstI     XbaI     XcmI
     XhoI     XmnI
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