Restriction map for pF1KE0501
 (Linear) (Six-base) MAPSORT of: KIEE0501.seq  Check: 1688  from: 1  to: 615



KIEE0501.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     615 
   Size:        142     473
 
AleI CACnn'nnGTG

Cuts at:      0     590     615 
   Size:        590      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     151     492     615 
   Size:        151     341     123

  Fragments arranged by size: 

                 341     151     123
 
ApaI G_GGCC'C

Cuts at:      0     110     537     615 
   Size:        110     427      78

  Fragments arranged by size: 

                 427     110      78
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     472     505     615 
   Size:        472      33     110

  Fragments arranged by size: 

                 472     110      33
 
BanI G'GyrC_C

Cuts at:      0     584     595     615 
   Size:        584      11      20

  Fragments arranged by size: 

                 584      20      11
 
BanII G_rGCy'C

Cuts at:      0     101     110     537     615 
   Size:        101       9     427      78

  Fragments arranged by size: 

                 427     101      78       9
 
BbvCI CC'TCA_GC

Cuts at:      0     280     615 
   Size:        280     335
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     577     615 
   Size:        577      38
 
Bme1580I G_kGCm'C

Cuts at:      0     110     537     615 
   Size:        110     427      78

  Fragments arranged by size: 

                 427     110      78
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     615 
   Size:        517      98
 
Bpu10I CC'TnA_GC

Cuts at:      0     280     615 
   Size:        280     335
 
BsaAI yAC'GTr

Cuts at:      0     449     615 
   Size:        449     166
 
BsaBI GATnn'nnATC

Cuts at:      0      58     367     615 
   Size:         58     309     248

  Fragments arranged by size: 

                 309     248      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     406     436     615 
   Size:        406      30     179

  Fragments arranged by size: 

                 406     179      30
 
BseYI C'CCAG_C

Cuts at:      0     242     388     615 
   Size:        242     146     227

  Fragments arranged by size: 

                 242     227     146
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     457     571     615 
   Size:        181     276     114      44

  Fragments arranged by size: 

                 276     181     114      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     101     320     615 
   Size:        101     219     295

  Fragments arranged by size: 

                 295     219     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     101     110     320     537     615 
   Size:        101       9     210     217      78

  Fragments arranged by size: 

                 217     210     101      78       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     136     500     615 
   Size:        136     364     115

  Fragments arranged by size: 

                 364     136     115
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     492     558     615 
   Size:        492      66      57

  Fragments arranged by size: 

                 492      66      57
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     303     403     615 
   Size:        303     100     212

  Fragments arranged by size: 

                 303     212     100
 
Bsu36I CC'TnA_GG

Cuts at:      0     289     615 
   Size:        289     326
 
BtgI C'CryG_G

Cuts at:      0     402     477     615 
   Size:        402      75     138

  Fragments arranged by size: 

                 402     138      75
 
BtsI GCAGTG_nn'

Cuts at:      0     586     615 
   Size:        586      29
 
EcoICRI GAG'CTC

Cuts at:      0      99     615 
   Size:         99     516
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     517     615 
   Size:        142     375      98

  Fragments arranged by size: 

                 375     142      98
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     160     615 
   Size:        160     455
 
EcoO109I rG'GnC_Cy

Cuts at:      0     107     533     534     615 
   Size:        107     426       1      81

  Fragments arranged by size: 

                 426     107      81       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     406     438     615 
   Size:        406      32     177

  Fragments arranged by size: 

                 406     177      32
 
MslI CAynn'nnrTG

Cuts at:      0      92     301     401     590     615 
   Size:         92     209     100     189      25

  Fragments arranged by size: 

                 209     189     100      92      25
 
MspA1I CmG'CkG

Cuts at:      0     242     615 
   Size:        242     373
 
NcoI C'CATG_G

Cuts at:      0     402     615 
   Size:        402     213
 
NspI r_CATG'y

Cuts at:      0     579     615 
   Size:        579      36
 
PasI CC'CwG_GG

Cuts at:      0     173     615 
   Size:        173     442
 
PmlI CAC'GTG

Cuts at:      0     449     615 
   Size:        449     166
 
PspOMI G'GGCC_C

Cuts at:      0     106     533     615 
   Size:        106     427      82

  Fragments arranged by size: 

                 427     106      82
 
PstI C_TGCA'G

Cuts at:      0     488     615 
   Size:        488     127
 
SacI G_AGCT'C

Cuts at:      0     101     615 
   Size:        101     514
 
SfcI C'TryA_G

Cuts at:      0     484     615 
   Size:        484     131
 
StyI C'CwwG_G

Cuts at:      0     402     615 
   Size:        402     213
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     165     615 
   Size:        165     450
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     161     193     615 
   Size:        161      32     422

  Fragments arranged by size: 

                 422     161      32
 
XmnI GAAnn'nnTTC

Cuts at:      0      77     615 
   Size:         77     538
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApaI     BaeI     BanI    BanII    BbvCI
    BciVI Bme1580I     BpmI   Bpu10I    BsaAI    BsaBI    BsaXI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI   BstAPI    BstXI   Bsu36I     BtgI
     BtsI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I     MslI   MspA1I
     NcoI     NspI     PasI     PmlI   PspOMI     PstI     SacI     SfcI
     StyI    TaqII     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BbeI     BbsI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
    BpuEI     BsaI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com