Restriction map for pF1KE0430
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MroI        MunI        MvnI        NarI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          730  GTAGAC               GT^[AC][GT]AC
AcsI          234  GAATTT               [GA]^AATT[CT]
Alw44I       1127  GTGCAC               G^TGCAC
AlwI         1041  GATCC                GGATC(4/5)
AocI          670  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1127  GTGCAC               G^TGCAC
ApoI          234  GAATTT               [GA]^AATT[CT]
AvrII         834  CCTAGG               C^CTAGG
BanI          436  GGCACC               G^G[CT][GA]CC
BlnI          834  CCTAGG               C^CTAGG
BsaI          259  GAGACC               GGTCTC(1/5)
BseRI         337  GAGGAG               GAGGAG(10/8)
BsgI          291  GTGCAG               GTGCAG(16/14)
BsrDI         690  GCAATG               GCAATG(2/0)
BsrFI         553  GCCGGC               [GA]^CCGG[CT]
BssSI         521  CACGAG               CACGAG(-5/-1)
BstXI         617  CCAGCCATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1040  AGATCC               [GA]^GATC[CT]
Bsu36I        670  CCTGAGG              CC^T[ACGT]AGG
CfoI         1221  GCGC                 GCG^C
Cfr10I        553  GCCGGC               [GA]^CCGG[CT]
DpnII        1041  GATC                 ^GATC
DsaI            7  CCATGG               C^C[GA][CT]GG
Eco81I        670  CCTGAGG              CC^T[ACGT]AGG
EcoNI         267  CCTGGTAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI         1221  GCGC                 GCG^C
HinP1I       1221  GCGC                 G^CGC
HincII        157  GTTGAC               GT[CT]^[GA]AC
HindII        157  GTTGAC               GT[CT]^[GA]AC
MboI         1041  GATC                 ^GATC
MflI         1040  AGATCC               [GA]^GATC[CT]
MseI          167  TTAA                 T^TAA
MslI         1023  CATCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI          553  GCCGGC               GCC^GGC
NcoI            7  CCATGG               C^CATGG
NdeII        1041  GATC                 ^GATC
NgoMIV        553  GCCGGC               G^CCGGC
PleI         1048  GAGTC                GAGTC(4/5)
PstI         1096  CTGCAG               CTGCA^G
PvuII          32  CAGCTG               CAG^CTG
Sau3AI       1041  GATC                 ^GATC
SfcI         1096  CTGCAG               C^T[GA][CT]AG
SfiI          577  GGCCAGTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI          721  CTCAAG               C^T[CT][GA]AG
Tru9I         167  TTAA                 T^TAA
XhoII        1040  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI         394  CAGTGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              665  CAGGACCTG           
BalI          576  TGGCCA               TGG^CCA
             1133  TGGCCA              
BbsI         1078  GAAGAC               GAAGAC(2/6)
              108  GTCTTC              
BbvCI          48  CCTCAGC              CCTCAGC(-5/-2)
              121  CCTCAGC             
BfaI           25  CTAG                 C^TAG
              835  CTAG                
BpuAI        1078  GAAGAC               GAAGAC(2/6)
              108  GTCTTC              
BspMI         142  ACCTGC               ACCTGC(4/8)
              891  GCAGGT              
MaeI           25  CTAG                 C^TAG
              835  CTAG                
MaeIII        542  GTGAC                ^GT[ACGT]AC
             1226  GTGAC               
MluNI         576  TGGCCA               TGG^CCA
             1133  TGGCCA              
MscI          576  TGGCCA               TGG^CCA
             1133  TGGCCA              
MspA1I         32  CAGCTG               C[AC]G^C[GT]G
              531  CAGCGG              
PpuMI          72  AGGACCT              [GA]G^G[AT]CC[CT]
              666  AGGACCT             
StuI          569  AGGCCT               AGG^CCT
              827  AGGCCT              
TaqI          308  TCGA                 T^CGA
              911  TCGA                
TfiI          409  GATTC                G^A[AT]TC
              676  GAATC               
Tsp45I        542  GTGAC                ^GT[GC]AC
             1226  GTGAC               
Tsp509I       235  AATT                 ^AATT
              779  AATT                
TthHB8I       308  TCGA                 T^CGA
              911  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        ApaI        AspHI       AvaII       
BanII       BbvI        BcnI        BglI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MboII       MnlI        MspI        MvaI        
MwoI        NciI        NlaIII      NlaIV       Sau96I      
ScrFI       SfaNI       StyI        TseI        TspRI       
XcmI        
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