Restriction map for pF1KE0430
 (Linear) (Six-base) MAPSORT of: KIEE0430.seq  Check: 9726  from: 1  to: 1251



KIEE0430.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     882    1251 
   Size:        882     369
 
AccI GT'mk_AC

Cuts at:      0     731    1251 
   Size:        731     520
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436    1251 
   Size:        436     815
 
AlwNI CAG_nnn'CTG

Cuts at:      0     399     670    1251 
   Size:        399     271     581

  Fragments arranged by size: 

                 581     399     271
 
ApaI G_GGCC'C

Cuts at:      0     381     449     711    1251 
   Size:        381      68     262     540

  Fragments arranged by size: 

                 540     381     262      68
 
ApaLI G'TGCA_C

Cuts at:      0    1127    1251 
   Size:       1127     124
 
ApoI r'AATT_y

Cuts at:      0     234    1251 
   Size:        234    1017
 
AvrII C'CTAG_G

Cuts at:      0     834    1251 
   Size:        834     417
 
BanI G'GyrC_C

Cuts at:      0     436    1251 
   Size:        436     815
 
BanII G_rGCy'C

Cuts at:      0     381     449     711     802     901    1251 
   Size:        381      68     262      91      99     350

  Fragments arranged by size: 

                 381     350     262      99      91      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     101    1085    1251 
   Size:        101     984     166

  Fragments arranged by size: 

                 984     166     101
 
BbvCI CC'TCA_GC

Cuts at:      0      49     122    1251 
   Size:         49      73    1129

  Fragments arranged by size: 

                1129      73      49
 
BglI GCCn_nnn'nGGC

Cuts at:      0     434     554     584    1141    1251 
   Size:        434     120      30     557     110

  Fragments arranged by size: 

                 557     434     120     110      30
 
Bme1580I G_kGCm'C

Cuts at:      0     381     449     539     657     711    1131    1251 
   Size:        381      68      90     118      54     420     120

  Fragments arranged by size: 

                 420     381     120     118      90      68      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      99    1251 
   Size:         99    1152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     199     301     346    1251 
   Size:         70     129     102      45     905

  Fragments arranged by size: 

                 905     129     102      70      45
 
Bpu10I CC'TnA_GC

Cuts at:      0      49     122     663    1148    1251 
   Size:         49      73     541     485     103

  Fragments arranged by size: 

                 541     485     103      73      49
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     706    1251 
   Size:        706     545
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253    1251 
   Size:        253     998
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     274     304     340     370    1251 
   Size:        274      30      36      30     881

  Fragments arranged by size: 

                 881     274      36      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     352    1251 
   Size:        352     899
 
BseYI C'CCAG_C

Cuts at:      0      30     704    1251 
   Size:         30     674     547

  Fragments arranged by size: 

                 674     547      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312    1251 
   Size:        312     939
 
BsiHKAI G_wGCw'C

Cuts at:      0      41     406    1131    1251 
   Size:         41     365     725     120

  Fragments arranged by size: 

                 725     365     120      41
 
Bsp1286I G_dGCh'C

Cuts at:      0      41     381     406     449     539     657     711     802 
   Size:         41     340      25      43      90     118      54      91

Cuts at:    802     901    1131    1251 
   Size:         99     230     120

  Fragments arranged by size: 

                 340     230     120     118      99      91      90      54
                  43      41      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     151     882    1251 
   Size:        151     731     369

  Fragments arranged by size: 

                 731     369     151
 
BsrDI GCAATG_nn'

Cuts at:      0     697    1251 
   Size:        697     554
 
BsrFI r'CCGG_y

Cuts at:      0     553    1251 
   Size:        553     698
 
BssSI C'ACGA_G

Cuts at:      0     521    1251 
   Size:        521     730
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     624    1251 
   Size:        624     627
 
BstYI r'GATC_y

Cuts at:      0    1040    1251 
   Size:       1040     211
 
Bsu36I CC'TnA_GG

Cuts at:      0     671    1251 
   Size:        671     580
 
BtgI C'CryG_G

Cuts at:      0       7    1251 
   Size:          7    1244
 
BtsI GCAGTG_nn'

Cuts at:      0     215     508     964    1230    1251 
   Size:        215     293     456     266      21

  Fragments arranged by size: 

                 456     293     266     215      21
 
EaeI y'GGCC_r

Cuts at:      0     576    1133    1193    1251 
   Size:        576     557      60      58

  Fragments arranged by size: 

                 576     557      60      58
 
EarI CTCTTCn'nnn_

Cuts at:      0      61     224     394    1251 
   Size:         61     163     170     857

  Fragments arranged by size: 

                 857     170     163      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     199     301     346     436    1251 
   Size:         70     129     102      45      90     815

  Fragments arranged by size: 

                 815     129     102      90      70      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     271    1251 
   Size:        271     980
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21      45      73     174     377     378     446     482 
   Size:         21      24      28     101     203       1      68      36

Cuts at:    482     667     708    1251 
   Size:        185      41     543

  Fragments arranged by size: 

                 543     203     185     101      68      41      36      28
                  24      21       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     321    1173    1251 
   Size:        321     852      78

  Fragments arranged by size: 

                 852     321      78
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     223     255    1251 
   Size:        223      32     996

  Fragments arranged by size: 

                 996     223      32
 
HincII GTy'rAC

Cuts at:      0     159    1251 
   Size:        159    1092
 
MscI TGG'CCA

Cuts at:      0     578    1135    1251 
   Size:        578     557     116

  Fragments arranged by size: 

                 578     557     116
 
MslI CAynn'nnrTG

Cuts at:      0    1027    1251 
   Size:       1027     224
 
MspA1I CmG'CkG

Cuts at:      0      34     533    1251 
   Size:         34     499     718

  Fragments arranged by size: 

                 718     499      34
 
NaeI GCC'GGC

Cuts at:      0     555    1251 
   Size:        555     696
 
NcoI C'CATG_G

Cuts at:      0       7    1251 
   Size:          7    1244
 
NgoMIV G'CCGG_C

Cuts at:      0     553    1251 
   Size:        553     698
 
PfoI T'CCnGG_A

Cuts at:      0     191     942    1251 
   Size:        191     751     309

  Fragments arranged by size: 

                 751     309     191
 
PpuMI rG'GwC_Cy

Cuts at:      0      73     667    1251 
   Size:         73     594     584

  Fragments arranged by size: 

                 594     584      73
 
PspOMI G'GGCC_C

Cuts at:      0     377     445     707    1251 
   Size:        377      68     262     544

  Fragments arranged by size: 

                 544     377     262      68
 
PstI C_TGCA'G

Cuts at:      0    1100    1251 
   Size:       1100     151
 
PvuII CAG'CTG

Cuts at:      0      34    1251 
   Size:         34    1217
 
SfcI C'TryA_G

Cuts at:      0    1096    1251 
   Size:       1096     155
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     584    1251 
   Size:        584     667
 
SmlI C'TyrA_G

Cuts at:      0     721    1251 
   Size:        721     530
 
StuI AGG'CCT

Cuts at:      0     571     829    1251 
   Size:        571     258     422

  Fragments arranged by size: 

                 571     422     258
 
StyI C'CwwG_G

Cuts at:      0       7     588     824     834    1251 
   Size:          7     581     236      10     417

  Fragments arranged by size: 

                 581     417     236      10       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     274     306     340     372    1251 
   Size:        274      32      34      32     879

  Fragments arranged by size: 

                 879     274      34      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      38      77     586    1251 
   Size:         38      39     509     665

  Fragments arranged by size: 

                 665     509      39      38
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AlwNI     ApaI    ApaLI     ApoI    AvrII
     BanI    BanII     BbsI    BbvCI     BglI Bme1580I     BmrI     BpmI
   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrDI    BsrFI    BssSI    BstXI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI  Eco57MI    EcoNI EcoO109I     FalI
    Hin4I   HincII     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV
     PfoI    PpuMI   PspOMI     PstI    PvuII     SfcI     SfiI     SmlI
     StuI     StyI     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     AscI     AseI    AsiSI     AvaI     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NarI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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