Restriction map for pF1KE0122
 (Linear) (Six-base) MAPSORT of: KIEE0122.seq  Check: 3726  from: 1  to: 795



KIEE0122.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

Acc65I G'GTAC_C

Cuts at:      0     473     795 
   Size:        473     322
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     795 
   Size:        265     530
 
AfeI AGC'GCT

Cuts at:      0     149     795 
   Size:        149     646
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     391     423     795 
   Size:        391      32     372

  Fragments arranged by size: 

                 391     372      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     294     351     630     732     795 
   Size:        294      57     279     102      63

  Fragments arranged by size: 

                 294     279     102      63      57
 
ApaLI G'TGCA_C

Cuts at:      0     484     795 
   Size:        484     311
 
AvaI C'yCGr_G

Cuts at:      0      45     419     795 
   Size:         45     374     376

  Fragments arranged by size: 

                 376     374      45
 
BamHI G'GATC_C

Cuts at:      0     273     795 
   Size:        273     522
 
BanI G'GyrC_C

Cuts at:      0      97     283     473     795 
   Size:         97     186     190     322

  Fragments arranged by size: 

                 322     190     186      97
 
BanII G_rGCy'C

Cuts at:      0      44     687     741     795 
   Size:         44     643      54      54

  Fragments arranged by size: 

                 643      54      54      44
 
BbeI G_GCGC'C

Cuts at:      0     101     795 
   Size:        101     694
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      28     795 
   Size:         28     767
 
Bme1580I G_kGCm'C

Cuts at:      0     288     488     795 
   Size:        288     200     307

  Fragments arranged by size: 

                 307     288     200
 
BmgBI CAC'GTC

Cuts at:      0     780     795 
   Size:        780      15
 
BmtI G_CTAG'C

Cuts at:      0     457     795 
   Size:        457     338
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     795 
   Size:        328     467
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     217     411     648     795 
   Size:        217     194     237     147

  Fragments arranged by size: 

                 237     217     194     147
 
BsaHI Gr'CG_yC

Cuts at:      0      98     795 
   Size:         98     697
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     391     421     442     472     795 
   Size:        391      30      21      30     323

  Fragments arranged by size: 

                 391     323      30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     282     463     763     795 
   Size:        282     181     300      32

  Fragments arranged by size: 

                 300     282     181      32
 
BseYI C'CCAG_C

Cuts at:      0     507     719     753     795 
   Size:        507     212      34      42

  Fragments arranged by size: 

                 507     212      42      34
 
BsiEI CG_ry'CG

Cuts at:      0     372     795 
   Size:        372     423
 
BsiHKAI G_wGCw'C

Cuts at:      0     426     488     795 
   Size:        426      62     307

  Fragments arranged by size: 

                 426     307      62
 
BsmI GAATG_Cn'

Cuts at:      0     595     795 
   Size:        595     200
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     288     426     488     687     741     795 
   Size:         44     244     138      62     199      54      54

  Fragments arranged by size: 

                 244     199     138      62      54      54      44
 
BsrBI CCG'CTC

Cuts at:      0     714     795 
   Size:        714      81
 
BsrFI r'CCGG_y

Cuts at:      0     100     160     795 
   Size:        100      60     635

  Fragments arranged by size: 

                 635     100      60
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     492     795 
   Size:        492     303
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     528     795 
   Size:        528     267
 
BstYI r'GATC_y

Cuts at:      0     273     290     795 
   Size:        273      17     505

  Fragments arranged by size: 

                 505     273      17
 
Bsu36I CC'TnA_GG

Cuts at:      0     253     733     795 
   Size:        253     480      62

  Fragments arranged by size: 

                 480     253      62
 
BtgI C'CryG_G

Cuts at:      0     167     658     795 
   Size:        167     491     137

  Fragments arranged by size: 

                 491     167     137
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     187     795 
   Size:        187     608
 
EaeI y'GGCC_r

Cuts at:      0     155     795 
   Size:        155     640
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     328     795 
   Size:        265      63     467

  Fragments arranged by size: 

                 467     265      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249     795 
   Size:        249     546
 
HaeII r_GCGC'y

Cuts at:      0     101     151     181     623     720     795 
   Size:        101      50      30     442      97      75

  Fragments arranged by size: 

                 442     101      97      75      50      30
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     258     290     795 
   Size:        258      32     505

  Fragments arranged by size: 

                 505     258      32
 
KasI G'GCGC_C

Cuts at:      0      97     795 
   Size:         97     698
 
KpnI G_GTAC'C

Cuts at:      0     477     795 
   Size:        477     318
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     636     795 
   Size:        636     159
 
MspA1I CmG'CkG

Cuts at:      0      57     138     169     471     795 
   Size:         57      81      31     302     324

  Fragments arranged by size: 

                 324     302      81      57      31
 
NaeI GCC'GGC

Cuts at:      0     102     162     795 
   Size:        102      60     633

  Fragments arranged by size: 

                 633     102      60
 
NarI GG'CG_CC

Cuts at:      0      98     795 
   Size:         98     697
 
NcoI C'CATG_G

Cuts at:      0     658     795 
   Size:        658     137
 
NgoMIV G'CCGG_C

Cuts at:      0     100     160     795 
   Size:        100      60     635

  Fragments arranged by size: 

                 635     100      60
 
NheI G'CTAG_C

Cuts at:      0     453     795 
   Size:        453     342
 
NruI TCG'CGA

Cuts at:      0     374     795 
   Size:        374     421
 
NspI r_CATG'y

Cuts at:      0     495     795 
   Size:        495     300
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     204     789     795 
   Size:        204     585       6

  Fragments arranged by size: 

                 585     204       6
 
PfoI T'CCnGG_A

Cuts at:      0     389     795 
   Size:        389     406
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     391     423     795 
   Size:        391      32     372

  Fragments arranged by size: 

                 391     372      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     249     795 
   Size:        249     546
 
PstI C_TGCA'G

Cuts at:      0     143     299     795 
   Size:        143     156     496

  Fragments arranged by size: 

                 496     156     143
 
SacII CC_GC'GG

Cuts at:      0     170     795 
   Size:        170     625
 
SbfI CC_TGCA'GG

Cuts at:      0     299     795 
   Size:        299     496
 
SfcI C'TryA_G

Cuts at:      0      52     139     295     795 
   Size:         52      87     156     500

  Fragments arranged by size: 

                 500     156      87      52
 
SfoI GGC'GCC

Cuts at:      0      99     795 
   Size:         99     696
 
SmaI CCC'GGG

Cuts at:      0      47     795 
   Size:         47     748
 
StyI C'CwwG_G

Cuts at:      0     658     795 
   Size:        658     137
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     358     441     580     621     786     795 
   Size:        358      83     139      41     165       9

  Fragments arranged by size: 

                 358     165     139      83      41       9
 
TatI w'GTAC_w

Cuts at:      0     704     795 
   Size:        704      91
 
Tth111I GACn'n_nGTC

Cuts at:      0     205     795 
   Size:        205     590
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     330     435     525     585     795 
   Size:        330     105      90      60     210

  Fragments arranged by size: 

                 330     210     105      90      60
 
XmaI C'CCGG_G

Cuts at:      0      45     795 
   Size:         45     750
 

 Enzymes that do cut:

   Acc65I     AcuI     AfeI     AloI    AlwNI    ApaLI     AvaI    BamHI
     BanI    BanII     BbeI     BcgI Bme1580I    BmgBI     BmtI     BpmI
     BsaI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BsrBI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     EaeI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     KpnI
     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NruI
     NspI    PflMI     PfoI     PpiI    PpuMI     PstI    SacII     SbfI
     SfcI     SfoI     SmaI     StyI    TaqII     TatI  Tth111I     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI
     BsgI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     MfeI     MluI     MscI     MslI     NdeI     NotI     NsiI
     PacI     PasI     PciI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XhoI     XmnI     ZraI
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