Restriction map for pF1KB7835
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        AspEI       
AspI        AvaI        AviII       BalI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BcnI        
BfrI        BglI        BglII       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HindIII     
HpaII       KasI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        345  GGTACC               G^GTACC
AflIII        122  ACATGT               A^C[GA][CT]GT
Alw44I        239  GTGCAC               G^TGCAC
ApaLI         239  GTGCAC               G^TGCAC
AseI          741  ATTAAT               AT^TAAT
AsnI          741  ATTAAT               AT^TAAT
Asp718I       345  GGTACC               G^GTACC
AvrII         406  CCTAGG               C^CTAGG
BamHI        1001  GGATCC               G^GATCC
BanII         764  GAGCTC               G[GA]GC[CT]^C
BcgI          537  GCAGCCTTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI          406  CCTAGG               C^CTAGG
BlpI         1606  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1606  GCTCAGC              GC^T[ACGT]AGC
BseRI        1355  GAGGAG               GAGGAG(10/8)
BsmBI        1358  GAGACG               CGTCTC(1/5)
BspLU11I      122  ACATGT               A^CATGT
BspMI        1577  ACCTGC               ACCTGC(4/8)
BstEII        727  GGTAACC              G^GT[ACGT]ACC
BstPI         727  GGTAACC              G^GT[ACGT]ACC
CelII        1606  GCTCAGC              GC^T[ACGT]AGC
EaeI         1308  TGGCCG               [CT]^GGCC[GA]
EcoNI        1478  CCTATGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       727  GGTAACC              G^GT[ACGT]ACC
EcoRI         449  GAATTC               G^AATTC
HgaI         1198  GCGTC                GACGC(5/10)
HpaI         1080  GTTAAC               GTT^AAC
KpnI          345  GGTACC               GGTAC^C
MspA1I        199  CAGCTG               C[AC]G^C[GT]G
PshBI         741  ATTAAT               AT^TAAT
PstI         1379  CTGCAG               CTGCA^G
PvuII         199  CAGCTG               CAG^CTG
SacI          764  GAGCTC               GAGCT^C
SapI          761  GAAGAGC              GCTCTTC(1/4)
ScaI         1259  AGTACT               AGT^ACT
SexAI          44  ACCAGGT              A^CC[AT]GGT
XcmI          423  CCACTCCCAGGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccI           97  GTCGAC               GT^[AC][GT]AC
             1223  GTCGAC              
Asp700I       832  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1321  GAAGACCTTC          
AspHI         239  GTGCAC               G[AT]GC[AT]^C
              764  GAGCTC              
BanI          345  GGTACC               G^G[CT][GA]CC
             1443  GGCACC              
BbsI         1321  GAAGAC               GAAGAC(2/6)
              138  GTCTTC              
BpuAI        1321  GAAGAC               GAAGAC(2/6)
              138  GTCTTC              
BsiHKAI       239  GTGCAC               G[AT]GC[AT]^C
              764  GAGCTC              
BsrI         1517  ACTGG                ACTGG(1/-1)
              135  CCAGT               
BstYI         867  AGATCC               [GA]^GATC[CT]
             1001  GGATCC              
DpnII         868  GATC                 ^GATC
             1002  GATC                
EarI         1330  CTCTTC               CTCTTC(1/4)
              761  GAAGAG              
EcoT14I       406  CCTAGG               C^C[AT][AT]GG
              752  CCTTGG              
HinfI         402  GAATC                G^A[ACGT]TC
              594  GAATC               
Ksp632I      1330  CTCTTC               CTCTTC(1/4)
              761  GAAGAG              
MaeII         326  ACGT                 A^CGT
             1361  ACGT                
MboI          868  GATC                 ^GATC
             1002  GATC                
MflI          867  AGATCC               [GA]^GATC[CT]
             1001  GGATCC              
NdeII         868  GATC                 ^GATC
             1002  GATC                
NspI           59  GCATGT               [GA]CATG^[CT]
              122  ACATGT              
SalI           97  GTCGAC               G^TCGAC
             1223  GTCGAC              
Sau3AI        868  GATC                 ^GATC
             1002  GATC                
SfaNI         178  GCATC                GCATC(5/9)
             1210  GCATC               
SfcI          129  CTACAG               C^T[GA][CT]AG
             1379  CTGCAG              
SmlI         1040  CTCAAG               C^T[CT][GA]AG
             1229  CTCAAG              
StuI          246  AGGCCT               AGG^CCT
             1155  AGGCCT              
StyI          406  CCTAGG               C^C[AT][AT]GG
              752  CCTTGG              
TfiI          402  GAATC                G^A[AT]TC
              594  GAATC               
TspRI         102  CAGTG                CA[GC]TG[ACGT][ACGT]^
             1382  CAGTG               
XhoII         867  AGATCC               [GA]^GATC[CT]
             1001  GGATCC              
XmnI          832  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1321  GAAGACCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AvaII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        EcoRII      Fnu4HI      FokI        HaeIII      
HincII      HindII      HphI        ItaI        MaeI        
MaeIII      MboII       MnlI        MseI        MvaI        
MwoI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TthHB8I     
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