Restriction map for pF1KE0494
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwNI       AocI        ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BseRI       BsiWI       BsmAI       
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrI        Bst1107I    
BstBI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
Eam1105I    Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        HincII      HindII      HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NaeI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SalI        SbfI        SexAI       
SfaNI       SfcI        SfiI        SfuI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TaqI        TfiI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AcyI          550  GGCGCC               G[GA]^CG[CT]C
Alw44I        309  GTGCAC               G^TGCAC
Aor51HI       486  AGCGCT               AGC^GCT
ApaI          330  GGGCCC               GGGCC^C
ApaLI         309  GTGCAC               G^TGCAC
AvaI          327  CCCGGG               C^[CT]CG[GA]G
BanI          550  GGCGCC               G^G[CT][GA]CC
BanII         330  GGGCCC               G[GA]GC[CT]^C
BbeI          550  GGCGCC               GGCGC^C
BbvCI         579  CCTCAGC              CCTCAGC(-5/-2)
BsaHI         550  GGCGCC               G[GA]^CG[CT]C
BsiEI         567  CGGCCG               CG[GA][CT]^CG
BsmFI         572  GTCCC                GGGAC(10/14)
BsoBI         327  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      491  TGTACA               T^GTACA
BsrGI         491  TGTACA               T^GTACA
BssHII        302  GCGCGC               G^CGCGC
BssSI         397  CTCGTG               CACGAG(-5/-1)
BstAPI        311  GCACCACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII         93  GGTGACC              G^GT[ACGT]ACC
BstF5I        152  GGATG                GGATG(2/0)
BstPI          93  GGTGACC              G^GT[ACGT]ACC
BstYI          20  AGATCC               [GA]^GATC[CT]
EagI          567  CGGCCG               C^GGCCG
EarI          283  CTCTTC               CTCTTC(1/4)
EclXI         567  CGGCCG               C^GGCCG
Eco47III      486  AGCGCT               AGC^GCT
Eco52I        567  CGGCCG               C^GGCCG
EcoO65I        93  GGTGACC              G^GT[ACGT]ACC
EcoT14I       338  CCAAGG               C^C[AT][AT]GG
FokI          152  GGATG                GGATG(9/13)
Hin1I         550  GGCGCC               G[GA]^CG[CT]C
HinfI         202  GACTC                G^A[ACGT]TC
KasI          550  GGCGCC               G^GCGCC
Ksp632I       283  CTCTTC               CTCTTC(1/4)
KspI          607  CCGCGG               CCGC^GG
MaeII         650  ACGT                 A^CGT
MflI           20  AGATCC               [GA]^GATC[CT]
MspA1I        607  CCGCGG               C[AC]G^C[GT]G
NarI          550  GGCGCC               GG^CGCC
NspI          182  ACATGC               [GA]CATG^[CT]
PleI          202  GACTC                GAGTC(4/5)
SacII         607  CCGCGG               CCGC^GG
SapI          282  GCTCTTC              GCTCTTC(1/4)
ScaI          596  AGTACT               AGT^ACT
SfoI          550  GGCGCC               GGC^GCC
SgrAI         624  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          327  CCCGGG               CCC^GGG
SspBI         491  TGTACA               T^GTACA
StyI          338  CCAAGG               C^C[AT][AT]GG
XcmI          338  CCAAGGCGCGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          20  AGATCC               [GA]^GATC[CT]
XmaCI         327  CCCGGG               C^CCGGG
XmaI          327  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          476  AGCT                 AG^CT
              583  AGCT                
AlwI           21  GATCC                GGATC(4/5)
               63  GATCC               
AvaII           5  GGTCC                G^G[AT]CC
              108  GGACC               
BspMI         316  ACCTGC               ACCTGC(4/8)
               90  GCAGGT              
BsrFI         565  ACCGGC               [GA]^CCGG[CT]
              625  ACCGGC              
BstXI         314  CCACCTGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              403  CCACTCTGCTGG        
Cfr10I        565  ACCGGC               [GA]^CCGG[CT]
              625  ACCGGC              
DpnII          21  GATC                 ^GATC
               63  GATC                
DsaI          607  CCGCGG               C^C[GA][CT]GG
              617  CCACGG              
HphI           93  GGTGA                GGTGA(8/7)
              248  TCACC               
MboI           21  GATC                 ^GATC
               63  GATC                
MboII         642  GAAGA                GAAGA(8/7)
              284  TCTTC               
MslI          222  CACCGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              315  CACCTGCGTG          
NdeII          21  GATC                 ^GATC
               63  GATC                
Sau3AI         21  GATC                 ^GATC
               63  GATC                
TspRI         187  CAGTG                CA[GC]TG[ACGT][ACGT]^
              307  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AfaI        AspHI       BbvI        
BcnI        BglI        BmyI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        Bsp1286I    BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        EaeI        EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MaeIII      MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       RsaI        Sau96I      ScrFI       TseI        
Tsp45I      
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