Restriction map for pF1KE0494
 (Linear) (Six-base) MAPSORT of: KIEE0494.seq  Check: 7195  from: 1  to: 654



KIEE0494.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      81     325     654 
   Size:         81     244     329

  Fragments arranged by size: 

                 329     244      81
 
AfeI AGC'GCT

Cuts at:      0     488     654 
   Size:        488     166
 
AleI CACnn'nnGTG

Cuts at:      0     226     319     654 
   Size:        226      93     335

  Fragments arranged by size: 

                 335     226      93
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     244     654 
   Size:        244     410
 
ApaI G_GGCC'C

Cuts at:      0     334     654 
   Size:        334     320
 
ApaLI G'TGCA_C

Cuts at:      0     309     654 
   Size:        309     345
 
AvaI C'yCGr_G

Cuts at:      0     327     654 
   Size:        327     327
 
BanI G'GyrC_C

Cuts at:      0     550     654 
   Size:        550     104
 
BanII G_rGCy'C

Cuts at:      0     334     654 
   Size:        334     320
 
BbeI G_GCGC'C

Cuts at:      0     554     654 
   Size:        554     100
 
BbvCI CC'TCA_GC

Cuts at:      0     580     654 
   Size:        580      74
 
BglI GCCn_nnn'nGGC

Cuts at:      0      47      87     612     654 
   Size:         47      40     525      42

  Fragments arranged by size: 

                 525      47      42      40
 
Bme1580I G_kGCm'C

Cuts at:      0     182     313     334     458     654 
   Size:        182     131      21     124     196

  Fragments arranged by size: 

                 196     182     131     124      21
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     385     654 
   Size:        385     269
 
Bpu10I CC'TnA_GC

Cuts at:      0     580     654 
   Size:        580      74
 
BsaHI Gr'CG_yC

Cuts at:      0     551     654 
   Size:        551     103
 
BseYI C'CCAG_C

Cuts at:      0      50     477     500     654 
   Size:         50     427      23     154

  Fragments arranged by size: 

                 427     154      50      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     250     546     654 
   Size:        187      63     296     108

  Fragments arranged by size: 

                 296     187     108      63
 
BsiEI CG_ry'CG

Cuts at:      0     570     654 
   Size:        570      84
 
BsiHKAI G_wGCw'C

Cuts at:      0     175     223     313     371     654 
   Size:        175      48      90      58     283

  Fragments arranged by size: 

                 283     175      90      58      48
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     182     223     313     334     371     458     654 
   Size:        175       7      41      90      21      37      87     196

  Fragments arranged by size: 

                 196     175      90      87      41      37      21       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      81     325     654 
   Size:         81     244     329

  Fragments arranged by size: 

                 329     244      81
 
BsrFI r'CCGG_y

Cuts at:      0     565     625     654 
   Size:        565      60      29

  Fragments arranged by size: 

                 565      60      29
 
BsrGI T'GTAC_A

Cuts at:      0     491     654 
   Size:        491     163
 
BssHII G'CGCG_C

Cuts at:      0     302     654 
   Size:        302     352
 
BssSI C'ACGA_G

Cuts at:      0     397     654 
   Size:        397     257
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     317     654 
   Size:        317     337
 
BstEII G'GTnAC_C

Cuts at:      0      93     654 
   Size:         93     561
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     321     410     654 
   Size:        321      89     244

  Fragments arranged by size: 

                 321     244      89
 
BstYI r'GATC_y

Cuts at:      0      20     654 
   Size:         20     634
 
BtgI C'CryG_G

Cuts at:      0     607     617     654 
   Size:        607      10      37

  Fragments arranged by size: 

                 607      37      10
 
BtsI GCAGTG_nn'

Cuts at:      0     193     313     654 
   Size:        193     120     341

  Fragments arranged by size: 

                 341     193     120
 
EaeI y'GGCC_r

Cuts at:      0     335     506     567     620     654 
   Size:        335     171      61      53      34

  Fragments arranged by size: 

                 335     171      61      53      34
 
EagI C'GGCC_G

Cuts at:      0     567     654 
   Size:        567      87
 
EarI CTCTTCn'nnn_

Cuts at:      0     289     654 
   Size:        289     365
 
HaeII r_GCGC'y

Cuts at:      0     247     260     397     490     554     607     654 
   Size:        247      13     137      93      64      53      47

  Fragments arranged by size: 

                 247     137      93      64      53      47      13
 
KasI G'GCGC_C

Cuts at:      0     550     654 
   Size:        550     104
 
MslI CAynn'nnrTG

Cuts at:      0     226     319     654 
   Size:        226      93     335

  Fragments arranged by size: 

                 335     226      93
 
MspA1I CmG'CkG

Cuts at:      0     609     654 
   Size:        609      45
 
NarI GG'CG_CC

Cuts at:      0     551     654 
   Size:        551     103
 
NspI r_CATG'y

Cuts at:      0     186     654 
   Size:        186     468
 
PfoI T'CCnGG_A

Cuts at:      0     104     654 
   Size:        104     550
 
PspOMI G'GGCC_C

Cuts at:      0     330     654 
   Size:        330     324
 
SacII CC_GC'GG

Cuts at:      0     610     654 
   Size:        610      44
 
SapI GCTCTTCn'nnn_

Cuts at:      0     289     654 
   Size:        289     365
 
ScaI AGT'ACT

Cuts at:      0     598     654 
   Size:        598      56
 
SfoI GGC'GCC

Cuts at:      0     552     654 
   Size:        552     102
 
SgrAI Cr'CCGG_yG

Cuts at:      0     625     654 
   Size:        625      29
 
SmaI CCC'GGG

Cuts at:      0     329     654 
   Size:        329     325
 
StyI C'CwwG_G

Cuts at:      0     338     654 
   Size:        338     316
 
TatI w'GTAC_w

Cuts at:      0     491     596     654 
   Size:        491     105      58

  Fragments arranged by size: 

                 491     105      58
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     555     587     654 
   Size:        555      32      67

  Fragments arranged by size: 

                 555      67      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     345     654 
   Size:        345     309
 
XmaI C'CCGG_G

Cuts at:      0     327     654 
   Size:        327     327
 

 Enzymes that do cut:

     AarI     AfeI     AleI     AlfI     ApaI    ApaLI     AvaI     BanI
    BanII     BbeI    BbvCI     BglI Bme1580I     BplI   Bpu10I    BsaHI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI     BtsI
     EaeI     EagI     EarI    HaeII     KasI     MslI   MspA1I     NarI
     NspI     PfoI   PspOMI    SacII     SapI     ScaI     SfoI    SgrAI
     SmaI     StyI     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BstBI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SgfI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII  Tth111I     XbaI     XhoI     XmnI     ZraI
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