Restriction map for pF1KB0998
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaII       AviII       BalI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlpI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DraI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       KasI        KpnI        Ksp632I     KspI        
MaeII       MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TaqI        Tru9I       
Tth111I     TthHB8I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AcsI          469  AAATTT               [GA]^AATT[CT]
ApoI          469  AAATTT               [GA]^AATT[CT]
AspHI         618  GAGCTC               G[AT]GC[AT]^C
AvaI          332  CCCGAG               C^[CT]CG[GA]G
AvrII          36  CCTAGG               C^CTAGG
BamHI         425  GGATCC               G^GATCC
BanII         618  GAGCTC               G[GA]GC[CT]^C
BbsI          316  GTCTTC               GAAGAC(2/6)
BfaI           37  CTAG                 C^TAG
BlnI           36  CCTAGG               C^CTAGG
BpuAI         316  GTCTTC               GAAGAC(2/6)
BseRI         270  CTCCTC               GAGGAG(10/8)
BsiHKAI       618  GAGCTC               G[AT]GC[AT]^C
BsmFI         434  GGGAC                GGGAC(10/14)
BsoBI         332  CCCGAG               C^[CT]CG[GA]G
BspMI         358  GCAGGT               ACCTGC(4/8)
BstAPI        302  GCATGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         425  GGATCC               [GA]^GATC[CT]
DpnII         426  GATC                 ^GATC
DraII         143  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        183  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      143  AGGGCCT              [GA]G^G[ACGT]CC[CT]
FauI          220  CCCGC                CCCGC(4/6)
HincII        635  GTCAAC               GT[CT]^[GA]AC
HindII        635  GTCAAC               GT[CT]^[GA]AC
MaeI           37  CTAG                 C^TAG
MboI          426  GATC                 ^GATC
MflI          425  GGATCC               [GA]^GATC[CT]
MslI          246  CATGATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII         426  GATC                 ^GATC
PleI          365  GAGTC                GAGTC(4/5)
PvuII          84  CAGCTG               CAG^CTG
SacI          618  GAGCTC               GAGCT^C
Sau3AI        426  GATC                 ^GATC
XcmI          338  CCAAAGACAGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         425  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AciI          221  CCGC                 CCGC(-3/-1)
              352  GCGG                
AlwI          426  GATCC                GGATC(4/5)
              425  GGATC               
BanI          109  GGTGCC               G^G[CT][GA]CC
              453  GGTGCC              
BbvI           75  GCTGC                GCAGC(8/12)
              235  GCTGC               
BmyI           57  GGGCAC               G[AGT]GC[ACT]^C
              618  GAGCTC              
Bsp1286I       57  GGGCAC               G[AGT]GC[ACT]^C
              618  GAGCTC              
Cfr13I        144  GGGCC                G^G[ACGT]CC
              213  GGGCC               
Fnu4HI         75  GCTGC                GC^[ACGT]GC
              235  GCTGC               
ItaI           75  GCTGC                GC^[ACGT]GC
              235  GCTGC               
MspA1I         84  CAGCTG               C[AC]G^C[GT]G
              221  CCGCTG              
PflMI          18  CCAACTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              625  CCAAGGCCTGG         
Sau96I        144  GGGCC                G^G[ACGT]CC
              213  GGGCC               
SfcI          198  CTATAG               C^T[GA][CT]AG
              669  CTACAG              
TseI           75  GCTGC                G^C[AT]GC
              235  GCTGC               
Tsp509I       470  AATT                 ^AATT
              664  AATT                
Van91I         18  CCAACTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              625  CCAAGGCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       EcoRII      
EcoT14I     FokI        HaeIII      HinfI       HphI        
MaeIII      MboII       MnlI        MvaI        MwoI        
NlaIII      NlaIV       ScrFI       SfaNI       StuI        
StyI        TfiI        Tsp45I      TspRI       
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