Restriction map for pF1KB6825
##################################
# NO CUT ENZYMES (  168 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BseAI       BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      CpoI        DraIII      
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
KasI        KpnI        MaeI        MamI        MfeI        
MflI        MluI        MroI        MslI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccII          97  CGCG                 CG^CG
AflII         223  CTTAAG               C^TTAAG
ApaI           47  GGGCCC               GGGCC^C
AvaII          81  GGACC                G^G[AT]CC
BalI          304  TGGCCA               TGG^CCA
BanII          47  GGGCCC               G[GA]GC[CT]^C
BcnI           84  CCCGG                CC^[GC]GG
BfrI          223  CTTAAG               C^TTAAG
BmyI           47  GGGCCC               G[AGT]GC[ACT]^C
BpmI           52  CTGGAG               CTGGAG(16/14)
BsaWI         103  TCCGGT               [AT]^CCGG[AT]
BsgI          282  CTGCAC               GTGCAG(16/14)
BsmAI         402  GAGAC                GTCTC(1/5)
Bsp1286I       47  GGGCCC               G[AGT]GC[ACT]^C
BspDI         339  ATCGAT               AT^CGAT
BsrBI          43  GAGCGG               CCGCTC(-3/-3)
BstF5I        450  CATCC                GGATG(2/0)
BstNI          51  CCTGG                CC^[AT]GG
BstUI          97  CGCG                 CG^CG
ClaI          339  ATCGAT               AT^CGAT
DpnII         338  GATC                 ^GATC
DraI          190  TTTAAA               TTT^AAA
DraII          47  GGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI           82  GACCCGGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI           96  CCGCGG               C^C[GA][CT]GG
EaeI          304  TGGCCA               [CT]^GGCC[GA]
EarI           28  GAAGAG               CTCTTC(1/4)
EcoO109I       47  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         148  GAATTC               G^AATTC
EcoRII         51  CCTGG                ^CC[AT]GG
FauI           45  GCGGG                CCCGC(4/6)
FokI          450  CATCC                GGATG(9/13)
HincII        211  GTTAAC               GT[CT]^[GA]AC
HindII        211  GTTAAC               GT[CT]^[GA]AC
HinfI         142  GAATC                G^A[ACGT]TC
HpaI          211  GTTAAC               GTT^AAC
HphI          113  TCACC                GGTGA(8/7)
Ksp632I        28  GAAGAG               CTCTTC(1/4)
KspI           96  CCGCGG               CCGC^GG
MboI          338  GATC                 ^GATC
MluNI         304  TGGCCA               TGG^CCA
MscI          304  TGGCCA               TGG^CCA
MvaI           51  CCTGG                CC^[AT]GG
MvnI           97  CGCG                 CG^CG
NciI           84  CCCGG                CC^[GC]GG
NdeII         338  GATC                 ^GATC
NlaIII        435  CATG                 CATG^
PvuII          61  CAGCTG               CAG^CTG
SacII          96  CCGCGG               CCGC^GG
Sau3AI        338  GATC                 ^GATC
ScaI           71  AGTACT               AGT^ACT
SfaNI         200  GCATC                GCATC(5/9)
TfiI          142  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AfaI           72  GTAC                 GT^AC
              247  GTAC                
BsaJI          50  CCCTGG               C^C[ACGT][ACGT]GG
               96  CCGCGG              
BsrI          166  ACTGG                ACTGG(1/-1)
              307  CCAGT               
BssKI          51  CCTGG                ^CC[ACGT]GG
               84  CCCGG               
HaeIII         48  GGCC                 GG^CC
              305  GGCC                
MaeII         110  ACGT                 A^CGT
              249  ACGT                
MaeIII        107  GTCAC                ^GT[ACGT]AC
              112  GTCAC               
MspA1I         61  CAGCTG               C[AC]G^C[GT]G
               96  CCGCGG              
MwoI          269  GCCTTTGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              387  GCTGTGCTTGC         
RsaI           72  GTAC                 GT^AC
              247  GTAC                
ScrFI          51  CCTGG                CC^[ACGT]GG
               84  CCCGG               
SmlI           67  CTCAAG               C^T[CT][GA]AG
              223  CTTAAG              
Tsp45I        107  GTCAC                ^GT[GC]AC
              112  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BseRI       Cac8I       Cfr13I      DdeI        Fnu4HI      
HapII       HpaII       ItaI        MboII       MnlI        
MseI        MspI        NlaIV       Sau96I      TaqI        
Tru9I       TseI        Tsp509I     TthHB8I     
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