Restriction map for pF1KE0486
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BanI        BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII         442  CGCG                 CG^CG
ApaI          519  GGGCCC               GGGCC^C
BalI          462  TGGCCA               TGG^CCA
BanII         519  GGGCCC               G[GA]GC[CT]^C
BbrPI         466  CACGTG               CAC^GTG
BglI          418  GCCACCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         452  AGATCT               A^GATCT
BsaAI         466  CACGTG               [CT]AC^GT[GA]
BseRI         244  GAGGAG               GAGGAG(10/8)
BsmI          332  GCATTC               GAATGC(1/-1)
BspMI         491  ACCTGC               ACCTGC(4/8)
BsrFI         338  ACCGGC               [GA]^CCGG[CT]
BsrI          167  CCAGT                ACTGG(1/-1)
BstUI         442  CGCG                 CG^CG
BstXI          50  CCATCTTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         452  AGATCT               [GA]^GATC[CT]
Cfr10I        338  ACCGGC               [GA]^CCGG[CT]
DraIII        343  CACACCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           77  CTCTTC               CTCTTC(1/4)
EcoT14I       422  CCATGG               C^C[AT][AT]GG
FauI          315  GCGGG                CCCGC(4/6)
HaeII         431  AGCGCC               [GA]GCGC^[CT]
Ksp632I        77  CTCTTC               CTCTTC(1/4)
MaeII         467  ACGT                 A^CGT
MaeIII        392  GTGAC                ^GT[ACGT]AC
MflI          452  AGATCT               [GA]^GATC[CT]
MluNI         462  TGGCCA               TGG^CCA
MscI          462  TGGCCA               TGG^CCA
MseI          209  TTAA                 T^TAA
MspA1I         88  CAGCTG               C[AC]G^C[GT]G
MvnI          442  CGCG                 CG^CG
NcoI          422  CCATGG               C^CATGG
PmaCI         466  CACGTG               CAC^GTG
PmlI          466  CACGTG               CAC^GTG
PpuMI         533  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          88  CAGCTG               CAG^CTG
SapI           76  GCTCTTC              GCTCTTC(1/4)
StyI          422  CCATGG               C^C[AT][AT]GG
TaqI          100  TCGA                 T^CGA
TfiI          174  GAATC                G^A[AT]TC
Tru9I         209  TTAA                 T^TAA
Tsp45I        392  GTGAC                ^GT[GC]AC
Tsp509I       186  AATT                 ^AATT
TthHB8I       100  TCGA                 T^CGA
XhoII         452  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AciI           94  GCGG                 CCGC(-3/-1)
              315  GCGG                
AvaII         135  GGTCC                G^G[AT]CC
              534  GGACC               
BcnI           31  CCCGG                CC^[GC]GG
              472  CCCGG               
BsmAI         230  GTCTC                GTCTC(1/5)
              478  GTCTC               
BstF5I         72  GGATG                GGATG(2/0)
              264  GGATG               
DpnII         195  GATC                 ^GATC
              453  GATC                
DsaI          347  CCGTGG               C^C[GA][CT]GG
              422  CCATGG              
EaeI           44  CGGCCA               [CT]^GGCC[GA]
              462  TGGCCA              
FokI           72  GGATG                GGATG(9/13)
              264  GGATG               
HphI          351  GGTGA                GGTGA(8/7)
              336  TCACC               
MboI          195  GATC                 ^GATC
              453  GATC                
MslI          259  CATGTGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              345  CACCGTGGTG          
NciI           31  CCCGG                CC^[GC]GG
              472  CCCGG               
NdeII         195  GATC                 ^GATC
              453  GATC                
NlaIII        259  CATG                 CATG^
              423  CATG                
PleI           97  GACTC                GAGTC(4/5)
              247  GAGTC               
Sau3AI        195  GATC                 ^GATC
              453  GATC                
TspRI         168  CAGTG                CA[GC]TG[ACGT][ACGT]^
              325  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AluI        AlwNI       BbvI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstNI       Cac8I       CfoI        Cfr13I      
DdeI        DraII       EcoO109I    EcoRII      Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        MboII       MnlI        MspI        
MvaI        MwoI        NlaIV       PstI        Sau96I      
ScrFI       SfaNI       SfcI        TseI        XcmI        
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