Restriction map for pF1KB8875
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        HphI        
KasI        Ksp632I     KspI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SapI        ScaI        SexAI       
SfiI        SfoI        SfuI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
SwaI        TaqI        Tru9I       Tsp509I     Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XhoII       
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        220  GGTACC               G^GTACC
AfaI          221  GTAC                 GT^AC
Asp718I       220  GGTACC               G^GTACC
AspHI         283  GAGCAC               G[AT]GC[AT]^C
AvaI           50  CCCGGG               C^[CT]CG[GA]G
BanII         449  GGGCTC               G[GA]GC[CT]^C
BcgI          158  GCACCGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          277  CTAG                 C^TAG
BsaBI         391  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          415  GGTCTC               GGTCTC(1/5)
BsaWI         217  TCCGGT               [AT]^CCGG[AT]
BseRI          10  GAGGAG               GAGGAG(10/8)
BsiEI          27  CGACCG               CG[GA][CT]^CG
BsiHKAI       283  GAGCAC               G[AT]GC[AT]^C
BsmAI         416  GTCTC                GTCTC(1/5)
BsmFI         404  GGGAC                GGGAC(10/14)
BsoBI          50  CCCGGG               C^[CT]CG[GA]G
BspHI         143  TCATGA               T^CATGA
BspMI         138  GCAGGT               ACCTGC(4/8)
BssSI         460  CTCGTG               CACGAG(-5/-1)
BstAPI         63  GCAGGAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         164  CCATCGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII         391  GATC                 ^GATC
DraIII        481  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I       425  CCTTGG               C^C[AT][AT]GG
HaeII         132  GGCGCT               [GA]GCGC^[CT]
HincII        298  GTCAAC               GT[CT]^[GA]AC
HindII        298  GTCAAC               GT[CT]^[GA]AC
KpnI          220  GGTACC               GGTAC^C
MaeI          277  CTAG                 C^TAG
MaeIII        353  GTCAC                ^GT[ACGT]AC
MamI          391  GATCTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          391  GATC                 ^GATC
MslI          357  CACTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           22  GCCGGC               GCC^GGC
NdeII         391  GATC                 ^GATC
NgoMIV         22  GCCGGC               G^CCGGC
PflMI         480  CCACATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         380  CAGCTG               CAG^CTG
RcaI          143  TCATGA               T^CATGA
RsaI          221  GTAC                 GT^AC
Sau3AI        391  GATC                 ^GATC
SbfI          471  CCTGCAGG             CCTGCA^GG
SfaNI          99  GCATC                GCATC(5/9)
SgrAI          21  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI           50  CCCGGG               CCC^GGG
Sse8387I      471  CCTGCAGG             CCTGCA^GG
StyI          425  CCTTGG               C^C[AT][AT]GG
Tsp45I        353  GTCAC                ^GT[GC]AC
Van91I        480  CCACATGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          50  CCCGGG               C^CCGGG
XmaI           50  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII          19  CGCG                 CG^CG
               88  CGCG                
AcyI          106  GACGCC               G[GA]^CG[CT]C
              406  GACGCC              
AvaII         273  GGTCC                G^G[AT]CC
              456  GGACC               
BpmI            7  CTGGAG               CTGGAG(16/14)
              172  CTGGAG              
BsaHI         106  GACGCC               G[GA]^CG[CT]C
              406  GACGCC              
BsgI           82  CTGCAC               GTGCAG(16/14)
               61  GTGCAG              
BsrFI          22  GCCGGC               [GA]^CCGG[CT]
               29  ACCGGC              
BstF5I        100  CATCC                GGATG(2/0)
              468  CATCC               
BstUI          19  CGCG                 CG^CG
               88  CGCG                
Cfr10I         22  GCCGGC               [GA]^CCGG[CT]
               29  ACCGGC              
DdeI           34  CTGAG                C^T[ACGT]AG
              452  CTCAG               
DraII         272  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              455  AGGACCT             
EcoO109I      272  AGGTCCT              [GA]G^G[ACGT]CC[CT]
              455  AGGACCT             
FauI          245  CCCGC                CCCGC(4/6)
              492  CCCGC               
FokI          100  CATCC                GGATG(9/13)
              468  CATCC               
Hin1I         106  GACGCC               G[GA]^CG[CT]C
              406  GACGCC              
HinfI         254  GATTC                G^A[ACGT]TC
              385  GAATC               
MaeII          59  ACGT                 A^CGT
              302  ACGT                
MspA1I        380  CAGCTG               C[AC]G^C[GT]G
              493  CCGCTG              
MvnI           19  CGCG                 CG^CG
               88  CGCG                
PpuMI         272  AGGTCCT              [GA]G^G[AT]CC[CT]
              455  AGGACCT             
TfiI          254  GATTC                G^A[AT]TC
              385  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AciI        AluI        BanI        BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        Bsp1286I    
BsrI        BssKI       BstNI       Cac8I       CfoI        
Cfr13I      EcoRII      Fnu4HI      HaeIII      HapII       
HgaI        HhaI        HinP1I      HpaII       ItaI        
MnlI        MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PstI        Sau96I      ScrFI       
SfcI        TseI        TspRI       
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