Restriction map for pF1KA1633
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccII       AccIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BbeI        BbvCI       BcgI        BlnI        BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrFI       
BssHII      BstUI       Bsu36I      Cfr10I      ClaI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        HpaI        KasI        KpnI        KspI        
MamI        MluI        MroI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmeI        PvuI        RcaI        SacII       SalI        
ScaI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       SpeI        SphI        SspI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AatII          25  GACGTC               GACGT^C
AclI         3012  AACGTT               AA^CGTT
AcyI           25  GACGTC               G[GA]^CG[CT]C
AvaI         5179  CCCGAG               C^[CT]CG[GA]G
BanI         2742  GGTGCC               G^G[CT][GA]CC
BbrPI        3544  CACGTG               CAC^GTG
BciVI        2239  GGATAC               GTATCC(6/5)
BcnI         2834  CCCGG                CC^[GC]GG
BsaHI          25  GACGTC               G[GA]^CG[CT]C
BsaI         4115  GAGACC               GGTCTC(1/5)
BsoBI        5179  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     2499  TGTACA               T^GTACA
BsrGI        2499  TGTACA               T^GTACA
Bst1107I     2001  GTATAC               GTA^TAC
BstBI        4205  TTCGAA               TT^CGAA
BstEII       5235  GGTCACC              G^GT[ACGT]ACC
BstPI        5235  GGTCACC              G^GT[ACGT]ACC
BstZ17I      2001  GTATAC               GTA^TAC
CpoI         3558  CGGTCCG              CG^G[AT]CCG
DraI         2932  TTTAAA               TTT^AAA
EcoO65I      5235  GGTCACC              G^GT[ACGT]ACC
HaeII        5312  AGCGCC               [GA]GCGC^[CT]
HapII        2835  CCGG                 C^CGG
Hin1I          25  GACGTC               G[GA]^CG[CT]C
HincII       3812  GTCAAC               GT[CT]^[GA]AC
HindII       3812  GTCAAC               GT[CT]^[GA]AC
HpaII        2835  CCGG                 C^CGG
MfeI         5338  CAATTG               C^AATTG
MslI         2452  CACCTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI         2835  CCGG                 C^CGG
MunI         5338  CAATTG               C^AATTG
NciI         2834  CCCGG                CC^[GC]GG
NheI         2113  GCTAGC               G^CTAGC
NspV         4205  TTCGAA               TT^CGAA
PmaCI        3544  CACGTG               CAC^GTG
PmlI         3544  CACGTG               CAC^GTG
PshAI        2081  GACTTGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     3012  AACGTT               AA^CGTT
RsrII        3558  CGGTCCG              CG^G[AT]CCG
SbfI         1737  CCTGCAGG             CCTGCA^GG
SfuI         4205  TTCGAA               TT^CGAA
Sse8387I     1737  CCTGCAGG             CCTGCA^GG
SspBI        2499  TGTACA               T^GTACA


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AccI         2001  GTATAC               GT^[AC][GT]AC
             2088  GTCTAC              
AflII        1957  CTTAAG               C^TTAAG
             2244  CTTAAG              
AflIII       1469  ACGTGT               A^C[GA][CT]GT
             3543  ACACGT              
ApaI         2282  GGGCCC               GGGCC^C
             3455  GGGCCC              
AseI         2161  ATTAAT               AT^TAAT
             3307  ATTAAT              
AsnI         2161  ATTAAT               AT^TAAT
             3307  ATTAAT              
BamHI         872  GGATCC               G^GATCC
             4405  GGATCC              
BfrI         1957  CTTAAG               C^TTAAG
             2244  CTTAAG              
BsaAI        3535  TACGTG               [CT]AC^GT[GA]
             3544  CACGTG              
BsmBI        2857  CGTCTC               CGTCTC(1/5)
              556  GAGACG              
BssSI         616  CACGAG               CACGAG(-5/-1)
             4112  CACGAG              
DrdI           25  GACGTCACCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3288  GACTGATCAGTC        
PpuMI         890  AGGACCT              [GA]G^G[AT]CC[CT]
             1259  AGGACCT             
PshBI        2161  ATTAAT               AT^TAAT
             3307  ATTAAT              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BalI        BanII       BbsI        BbvI        BclI        
BfaI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspMI       BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstXI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        HaeIII      HgaI        HhaI        HinP1I      
HindIII     HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MspA1I      MvaI        MwoI        NcoI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RsaI        SacI        SapI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmnI        
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