Restriction map for pF1KA1633
 (Linear) (Six-base) MAPSORT of: KIAA1633.seq  Check: 5148  from: 1  to: 5442



KIAA1633.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     400     453    5442 
   Size:        400      53    4989

  Fragments arranged by size: 

                4989     400      53
 
AatII G_ACGT'C

Cuts at:      0      29    5442 
   Size:         29    5413
 
AccI GT'mk_AC

Cuts at:      0    2002    2089    5442 
   Size:       2002      87    3353

  Fragments arranged by size: 

                3353    2002      87
 
AclI AA'CG_TT

Cuts at:      0    3013    5442 
   Size:       3013    2429
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     425    1118    1462    2041    2566    2944    2996 
   Size:        358      67     693     344     579     525     378      52

Cuts at:   2996    3212    3227    4043    4133    4465    4639    4702    5173 
   Size:        216      15     816      90     332     174      63     471

Cuts at:   5173    5442 
   Size:        269

  Fragments arranged by size: 

                 816     693     579     525     471     378     358     344
                 332     269     216     174      90      67      63      52
                  15
 
AflII C'TTAA_G

Cuts at:      0    1957    2244    5442 
   Size:       1957     287    3198

  Fragments arranged by size: 

                3198    1957     287
 
AflIII A'CryG_T

Cuts at:      0    1469    3543    5442 
   Size:       1469    2074    1899

  Fragments arranged by size: 

                2074    1899    1469
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2313    5442 
   Size:       2313    3129
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    5055    5087    5406    5438    5442 
   Size:       5055      32     319      32       4

  Fragments arranged by size: 

                5055     319      32      32       4
 
AlwNI CAG_nnn'CTG

Cuts at:      0      53    1221    2019    3615    4563    4596    4635    5442 
   Size:         53    1168     798    1596     948      33      39     807

  Fragments arranged by size: 

                1596    1168     948     807     798      53      39      33
            
 
ApaI G_GGCC'C

Cuts at:      0    2286    3459    5442 
   Size:       2286    1173    1983

  Fragments arranged by size: 

                2286    1983    1173
 
ApoI r'AATT_y

Cuts at:      0     271    1084    1483    1806    1841    3381    3651    4202 
   Size:        271     813     399     323      35    1540     270     551

Cuts at:   4202    4285    4352    5442 
   Size:         83      67    1090

  Fragments arranged by size: 

                1540    1090     813     551     399     323     271     270
                  83      67      35
 
AseI AT'TA_AT

Cuts at:      0    2162    3308    5442 
   Size:       2162    1146    2134

  Fragments arranged by size: 

                2162    2134    1146
 
AvaI C'yCGr_G

Cuts at:      0    5179    5442 
   Size:       5179     263
 
BamHI G'GATC_C

Cuts at:      0     872    4405    5442 
   Size:        872    3533    1037

  Fragments arranged by size: 

                3533    1037     872
 
BanI G'GyrC_C

Cuts at:      0    2742    5442 
   Size:       2742    2700
 
BanII G_rGCy'C

Cuts at:      0     317     776    1553    2286    3223    3459    3758    5406 
   Size:        317     459     777     733     937     236     299    1648

Cuts at:   5406    5442 
   Size:         36

  Fragments arranged by size: 

                1648     937     777     733     459     317     299     236
                  36
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1106    1381    1822    2479    4019    4150    4465    5442 
   Size:       1106     275     441     657    1540     131     315     977

  Fragments arranged by size: 

                1540    1106     977     657     441     315     275     131
            
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2233    5442 
   Size:       2233    3209
 
BclI T'GATC_A

Cuts at:      0    1433    1881    3123    3291    5334    5442 
   Size:       1433     448    1242     168    2043     108

  Fragments arranged by size: 

                2043    1433    1242     448     168     108
 
BglI GCCn_nnn'nGGC

Cuts at:      0     523    1057    1138    2751    5442 
   Size:        523     534      81    1613    2691

  Fragments arranged by size: 

                2691    1613     534     523      81
 
BglII A'GATC_T

Cuts at:      0     471    2900    3705    5442 
   Size:        471    2429     805    1737

  Fragments arranged by size: 

                2429    1737     805     471
 
BlpI GC'TnA_GC

Cuts at:      0      46     399    3026    3917    4597    5305    5442 
   Size:         46     353    2627     891     680     708     137

  Fragments arranged by size: 

                2627     891     708     680     353     137      46
 
Bme1580I G_kGCm'C

Cuts at:      0    2286    2591    3459    5197    5442 
   Size:       2286     305     868    1738     245

  Fragments arranged by size: 

                2286    1738     868     305     245
 
BmgBI CAC'GTC

Cuts at:      0    1470    5442 
   Size:       1470    3972
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      65    3405    5442 
   Size:         65    3340    2037

  Fragments arranged by size: 

                3340    2037      65
 
BmtI G_CTAG'C

Cuts at:      0    2117    5442 
   Size:       2117    3325
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3165    4465    4467    5191    5442 
   Size:       3165    1300       2     724     251

  Fragments arranged by size: 

                3165    1300     724     251       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     788    1273    1285    1801    2465    3120    3616 
   Size:        334     454     485      12     516     664     655     496

Cuts at:   3616    3637    3946    4437    4492    5184    5404    5442 
   Size:         21     309     491      55     692     220      38

  Fragments arranged by size: 

                 692     664     655     516     496     491     485     454
                 334     309     220      55      38      21      12
 
Bpu10I CC'TnA_GC

Cuts at:      0    1552    2480    4052    5389    5442 
   Size:       1552     928    1572    1337      53

  Fragments arranged by size: 

                1572    1552    1337     928      53
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271     301    1567    1931    3489    4157    4383    5442 
   Size:        271      30    1266     364    1558     668     226    1059

  Fragments arranged by size: 

                1558    1266    1059     668     364     271     226      30
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4109    5442 
   Size:       4109    1333
 
BsaAI yAC'GTr

Cuts at:      0    3537    3546    5442 
   Size:       3537       9    1896

  Fragments arranged by size: 

                3537    1896       9
 
BsaHI Gr'CG_yC

Cuts at:      0      26    5442 
   Size:         26    5416
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     141     171    4606    4636    4825    4831    4855    4861 
   Size:        141      30    4435      30     189       6      24       6

Cuts at:   4861    4863    4893    4985    5015    5442 
   Size:          2      30      92      30     427

  Fragments arranged by size: 

                4435     427     189     141      92      30      30      30
                  30      24       6       6       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     685     739    1840    3859    4627    4805    5442 
   Size:        685      54    1101    2019     768     178     637

  Fragments arranged by size: 

                2019    1101     768     685     637     178      54
 
BseYI C'CCAG_C

Cuts at:      0     109    2564    5038    5058    5079    5425    5442 
   Size:        109    2455    2474      20      21     346      17

  Fragments arranged by size: 

                2474    2455     346     109      21      20      17
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     812    1284    2638    2687    2719    3435    4627 
   Size:        478     334     472    1354      49      32     716    1192

Cuts at:   4627    5442 
   Size:        815

  Fragments arranged by size: 

                1354    1192     815     716     478     472     334      49
                  32
 
BsiHKAI G_wGCw'C

Cuts at:      0     171     317     776    1553    1585    3758    4478    4712 
   Size:        171     146     459     777      32    2173     720     234

Cuts at:   4712    5116    5442 
   Size:        404     326

  Fragments arranged by size: 

                2173     777     720     459     404     326     234     171
                 146      32
 
BsmI GAATG_Cn'

Cuts at:      0     266    2314    3027    5442 
   Size:        266    2048     713    2415

  Fragments arranged by size: 

                2415    2048     713     266
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     550    2863    5442 
   Size:        550    2313    2579

  Fragments arranged by size: 

                2579    2313     550
 
Bsp1286I G_dGCh'C

Cuts at:      0     171     317     776    1553    1585    2286    2591    3223 
   Size:        171     146     459     777      32     701     305     632

Cuts at:   3223    3459    3758    4478    4712    5116    5197    5406    5442 
   Size:        236     299     720     234     404      81     209      36

  Fragments arranged by size: 

                 777     720     701     632     459     404     305     299
                 236     234     209     171     146      81      36      32
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     400     453    1745    2333    2651    2741    5442 
   Size:        400      53    1292     588     318      90    2701

  Fragments arranged by size: 

                2701    1292     588     400     318      90      53
 
BsrDI GCAATG_nn'

Cuts at:      0    1347    1373    4397    5442 
   Size:       1347      26    3024    1045

  Fragments arranged by size: 

                3024    1347    1045      26
 
BsrGI T'GTAC_A

Cuts at:      0    2499    5442 
   Size:       2499    2943
 
BssSI C'ACGA_G

Cuts at:      0     616    4112    5442 
   Size:        616    3496    1330

  Fragments arranged by size: 

                3496    1330     616
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1791    2742    3528    4635    5442 
   Size:       1791     951     786    1107     807

  Fragments arranged by size: 

                1791    1107     951     807     786
 
BstBI TT'CG_AA

Cuts at:      0    4206    5442 
   Size:       4206    1236
 
BstEII G'GTnAC_C

Cuts at:      0    5235    5442 
   Size:       5235     207
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2065    2293    3867    5442 
   Size:       2065     228    1574    1575

  Fragments arranged by size: 

                2065    1575    1574     228
 
BstYI r'GATC_y

Cuts at:      0     471     872    1542    2900    3677    3705    4279    4405 
   Size:        471     401     670    1358     777      28     574     126

Cuts at:   4405    5442 
   Size:       1037

  Fragments arranged by size: 

                1358    1037     777     670     574     471     401     126
                  28
 
BstZ17I GTA'TAC

Cuts at:      0    2003    5442 
   Size:       2003    3439
 
BtgI C'CryG_G

Cuts at:      0     971    2630    3789    4742    5442 
   Size:        971    1659    1159     953     700

  Fragments arranged by size: 

                1659    1159     971     953     700
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     646    5442 
   Size:        646    4796
 
BtsI GCAGTG_nn'

Cuts at:      0      63    2699    2987    3423    4793    5112    5442 
   Size:         63    2636     288     436    1370     319     330

  Fragments arranged by size: 

                2636    1370     436     330     319     288      63
 
DraI TTT'AAA

Cuts at:      0    2934    5442 
   Size:       2934    2508
 
DraIII CAC_nnn'GTG

Cuts at:      0    2742    3528    4883    5442 
   Size:       2742     786    1355     559

  Fragments arranged by size: 

                2742    1355     786     559
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      31    3294    5442 
   Size:         31    3263    2148

  Fragments arranged by size: 

                3263    2148      31
 
EaeI y'GGCC_r

Cuts at:      0    2627    2722    3786    3881    4587    4905    4932    5117 
   Size:       2627      95    1064      95     706     318      27     185

Cuts at:   5117    5187    5442 
   Size:         70     255

  Fragments arranged by size: 

                2627    1064     706     318     255     185      95      95
                  70      27
 
EarI CTCTTCn'nnn_

Cuts at:      0      14     575     685     881    1279    1590    1646    2678 
   Size:         14     561     110     196     398     311      56    1032

Cuts at:   2678    4700    5129    5442 
   Size:       2022     429     313

  Fragments arranged by size: 

                2022    1032     561     429     398     313     311     196
                 110      56      14
 
EcoICRI GAG'CTC

Cuts at:      0     315     774    1551    3756    5442 
   Size:        315     459     777    2205    1686

  Fragments arranged by size: 

                2205    1686     777     459     315
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     358     425     788    1118    1273    1285    1462 
   Size:        334      24      67     363     330     155      12     177

Cuts at:   1462    1801    2041    2465    2566    2944    2996    3120    3212 
   Size:        339     240     424     101     378      52     124      92

Cuts at:   3212    3227    3616    3637    3946    4043    4133    4437    4465 
   Size:         15     389      21     309      97      90     304      28

Cuts at:   4465    4492    4639    4702    5173    5184    5404    5442 
   Size:         27     147      63     471      11     220      38

  Fragments arranged by size: 

                 471     424     389     378     363     339     334     330
                 309     304     240     220     177     155     147     124
                 101      97      92      90      67      63      52      38
                  28      27      24      21      15      12      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     721    4954    5134    5365    5442 
   Size:        721    4233     180     231      77

  Fragments arranged by size: 

                4233     721     231     180      77
 
EcoO109I rG'GnC_Cy

Cuts at:      0     891    1059    1260    2282    3455    4380    4968    5442 
   Size:        891     168     201    1022    1173     925     588     474

  Fragments arranged by size: 

                1173    1022     925     891     588     474     201     168
            
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1972    2512    2693    2826    4122    5442 
   Size:       1972     540     181     133    1296    1320

  Fragments arranged by size: 

                1972    1320    1296     540     181     133
 
HaeII r_GCGC'y

Cuts at:      0    5316    5442 
   Size:       5316     126
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     715     729     745     747     761     777    1425    1457 
   Size:        715      14      16       2      14      16     648      32

Cuts at:   1457    1873    1905    3669    3701    4825    4831    4857    4863 
   Size:        416      32    1764      32    1124       6      26       6

Cuts at:   4863    4985    5017    5442 
   Size:        122      32     425

  Fragments arranged by size: 

                1764    1124     715     648     425     416     122      32
                  32      32      32      26      16      16      14      14
                   6       6       2
 
HincII GTy'rAC

Cuts at:      0    3814    5442 
   Size:       3814    1628
 
HindIII A'AGCT_T

Cuts at:      0     592     701    5155    5293    5442 
   Size:        592     109    4454     138     149

  Fragments arranged by size: 

                4454     592     149     138     109
 
MfeI C'AATT_G

Cuts at:      0    5338    5442 
   Size:       5338     104
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    3945    4247    5442 
   Size:       3945     302    1195

  Fragments arranged by size: 

                3945    1195     302
 
MscI TGG'CCA

Cuts at:      0    2629    3788    4907    5189    5442 
   Size:       2629    1159    1119     282     253

  Fragments arranged by size: 

                2629    1159    1119     282     253
 
MslI CAynn'nnrTG

Cuts at:      0    2456    5442 
   Size:       2456    2986
 
MspA1I CmG'CkG

Cuts at:      0      50     366     429    2889    3564    3615    3903    4485 
   Size:         50     316      63    2460     675      51     288     582

Cuts at:   4485    4563    4580    4586    4635    5304    5442 
   Size:         78      17       6      49     669     138

  Fragments arranged by size: 

                2460     675     669     582     316     288     138      78
                  63      51      50      49      17       6
 
NcoI C'CATG_G

Cuts at:      0     971    3789    4742    5442 
   Size:        971    2818     953     700

  Fragments arranged by size: 

                2818     971     953     700
 
NheI G'CTAG_C

Cuts at:      0    2113    5442 
   Size:       2113    3329
 
NspI r_CATG'y

Cuts at:      0     746    3663    5420    5442 
   Size:        746    2917    1757      22

  Fragments arranged by size: 

                2917    1757     746      22
 
PasI CC'CwG_GG

Cuts at:      0      37    5442 
   Size:         37    5405
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2877    2904    3354    4825    4944    5442 
   Size:       2877      27     450    1471     119     498

  Fragments arranged by size: 

                2877    1471     498     450     119      27
 
PfoI T'CCnGG_A

Cuts at:      0     350     644    3893    4453    4568    5409    5442 
   Size:        350     294    3249     560     115     841      33

  Fragments arranged by size: 

                3249     841     560     350     294     115      33
 
PmlI CAC'GTG

Cuts at:      0    3546    5442 
   Size:       3546    1896
 
PpuMI rG'GwC_Cy

Cuts at:      0     891    1260    5442 
   Size:        891     369    4182

  Fragments arranged by size: 

                4182     891     369
 
PshAI GACnn'nnGTC

Cuts at:      0    2085    5442 
   Size:       2085    3357
 
PspOMI G'GGCC_C

Cuts at:      0    2282    3455    5442 
   Size:       2282    1173    1987

  Fragments arranged by size: 

                2282    1987    1173
 
PstI C_TGCA'G

Cuts at:      0      58    1539    1715    1742    2894    2915    3641    3692 
   Size:         58    1481     176      27    1152      21     726      51

Cuts at:   3692    3857    4526    4585    5442 
   Size:        165     669      59     857

  Fragments arranged by size: 

                1481    1152     857     726     669     176     165      59
                  58      51      27      21
 
PvuII CAG'CTG

Cuts at:      0     366    2889    3615    3903    4563    4635    5304    5442 
   Size:        366    2523     726     288     660      72     669     138

  Fragments arranged by size: 

                2523     726     669     660     366     288     138      72
            
 
RsrII CG'GwC_CG

Cuts at:      0    3559    5442 
   Size:       3559    1883
 
SacI G_AGCT'C

Cuts at:      0     317     776    1553    3758    5442 
   Size:        317     459     777    2205    1684

  Fragments arranged by size: 

                2205    1684     777     459     317
 
SapI GCTCTTCn'nnn_

Cuts at:      0     575     685    1279    1590    1646    4700    5442 
   Size:        575     110     594     311      56    3054     742

  Fragments arranged by size: 

                3054     742     594     575     311     110      56
 
SbfI CC_TGCA'GG

Cuts at:      0    1742    5442 
   Size:       1742    3700
 
SfcI C'TryA_G

Cuts at:      0      54     920    1063    1535    1711    1738    2090    2890 
   Size:         54     866     143     472     176      27     352     800

Cuts at:   2890    2911    3637    3688    3853    4522    4581    5142    5442 
   Size:         21     726      51     165     669      59     561     300

  Fragments arranged by size: 

                 866     800     726     669     561     472     352     300
                 176     165     143      59      54      51      27      21
            
 
SmlI C'TyrA_G

Cuts at:      0     250     316    1546    1946    1957    2244    3468    4172 
   Size:        250      66    1230     400      11     287    1224     704

Cuts at:   4172    4362    5442 
   Size:        190    1080

  Fragments arranged by size: 

                1230    1224    1080     704     400     287     250     190
                  66      11
 
StuI AGG'CCT

Cuts at:      0     541    5140    5394    5442 
   Size:        541    4599     254      48

  Fragments arranged by size: 

                4599     541     254      48
 
StyI C'CwwG_G

Cuts at:      0     796     971    1820    2591    3789    4742    5442 
   Size:        796     175     849     771    1198     953     700

  Fragments arranged by size: 

                1198     953     849     796     771     700     175
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1228    2738    2884    3547    3997    5093    5442 
   Size:       1228    1510     146     663     450    1096     349

  Fragments arranged by size: 

                1510    1228    1096     663     450     349     146
 
TatI w'GTAC_w

Cuts at:      0    1171    2499    5442 
   Size:       1171    1328    2943

  Fragments arranged by size: 

                2943    1328    1171
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    5176    5208    5442 
   Size:       5176      32     234

  Fragments arranged by size: 

                5176     234      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111    2361    2874    5127    5379    5442 
   Size:        111    2250     513    2253     252      63

  Fragments arranged by size: 

                2253    2250     513     252     111      63
 
XmnI GAAnn'nnTTC

Cuts at:      0    2405    3212    3697    4289    5442 
   Size:       2405     807     485     592    1153

  Fragments arranged by size: 

                2405    1153     807     592     485
 
ZraI GAC'GTC

Cuts at:      0      27    5442 
   Size:         27    5415
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI    AflII   AflIII     AlfI
     AloI    AlwNI     ApaI     ApoI     AseI     AvaI    BamHI     BanI
    BanII     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspMI    BsrDI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI     DraI
   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FalI    HaeII    Hin4I   HincII  HindIII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NheI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI   PspOMI     PstI    PvuII    RsrII     SacI
     SapI     SbfI     SfcI     SmlI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI     AgeI     AhdI     AleI    ApaLI     AscI    AsiSI
    AvrII     BaeI     BbeI    BbvCI     BcgI    BfrBI    BsaBI    BsaWI
    BsiEI    BsiWI    BspEI    BspHI    BsrBI    BsrFI   BssHII   Bsu36I
     ClaI    CspCI     EagI     EciI    EcoRI    EcoRV     FseI     FspI
    FspAI     HpaI     KasI     KpnI     MluI     NaeI     NarI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI
     PsiI    PspXI     PsrI     PvuI    SacII     SalI    SanDI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     XmaI
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