Restriction map for pF1KB6129
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BanI        BbeI        BbrPI       BciVI       
BclI        BglI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XcmI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          304  GTCTAC               GT^[AC][GT]AC
AcsI          536  GAATTC               [GA]^AATT[CT]
AflII        1591  CTTAAG               C^TTAAG
AlwNI         874  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          223  CCTCAGG              CC^T[ACGT]AGG
ApoI          536  GAATTC               [GA]^AATT[CT]
AspHI         149  GAGCTC               G[AT]GC[AT]^C
BamHI        1064  GGATCC               G^GATCC
BfrI         1591  CTTAAG               C^TTAAG
BlpI          451  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      451  GCTGAGC              GC^T[ACGT]AGC
BsiHKAI       149  GAGCTC               G[AT]GC[AT]^C
BsmBI        1303  GAGACG               CGTCTC(1/5)
BsrBI         510  CCGCTC               CCGCTC(-3/-3)
BsrDI        1284  CATTGC               GCAATG(2/0)
Bsu36I        223  CCTCAGG              CC^T[ACGT]AGG
CelII         451  GCTGAGC              GC^T[ACGT]AGC
CfoI          731  GCGC                 GCG^C
DraI          204  TTTAAA               TTT^AAA
DraII         626  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eco81I        223  CCTCAGG              CC^T[ACGT]AGG
EcoNI         399  CCTCCAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      626  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         536  GAATTC               G^AATTC
EcoT14I      1088  CCAAGG               C^C[AT][AT]GG
FauI          509  CCCGC                CCCGC(4/6)
HaeII         730  AGCGCC               [GA]GCGC^[CT]
HhaI          731  GCGC                 GCG^C
HinP1I        731  GCGC                 G^CGC
MspA1I       1114  CAGCTG               C[AC]G^C[GT]G
PaeR7I        310  CTCGAG               C^TCGAG
PpuMI         626  GGGACCT              [GA]G^G[AT]CC[CT]
PvuII        1114  CAGCTG               CAG^CTG
SacI          149  GAGCTC               GAGCT^C
StyI         1088  CCAAGG               C^C[AT][AT]GG
TaqI          311  TCGA                 T^CGA
TthHB8I       311  TCGA                 T^CGA
XbaI          846  TCTAGA               T^CTAGA
XhoI          310  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AflIII        779  ACATGT               A^C[GA][CT]GT
             1716  ACATGT              
Asp700I       156  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1249  GAATCTATTC          
BcgI          696  CGAGACCTATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1278  CGATTTCATTGC        
BpmI          168  CTCCAG               CTGGAG(16/14)
             1041  CTCCAG              
BspHI         269  TCATGA               T^CATGA
             1122  TCATGA              
BspLU11I      779  ACATGT               A^CATGT
             1716  ACATGT              
BspMI         383  ACCTGC               ACCTGC(4/8)
             1154  ACCTGC              
HindIII       862  AAGCTT               A^AGCTT
             1217  AAGCTT              
PstI         1111  CTGCAG               CTGCA^G
             1725  CTGCAG              
RcaI          269  TCATGA               T^CATGA
             1122  TCATGA              
SfaNI         616  GATGC                GCATC(5/9)
             1609  GATGC               
SmaI          515  CCCGGG               CCC^GGG
              809  CCCGGG              
SmlI          310  CTCGAG               C^T[CT][GA]AG
             1591  CTTAAG              
XmaCI         515  CCCGGG               C^CCGGG
              809  CCCGGG              
XmaI          515  CCCGGG               C^CCGGG
              809  CCCGGG              
XmnI          156  GAAGGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1249  GAATCTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
AvaII       BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BmyI        BpuAI       BsaI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EarI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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