Restriction map for pF1KB6129
 (Linear) (Six-base) MAPSORT of: KIBB6129.seq  Check: 4603  from: 1  to: 1761



KIBB6129.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1163    1761 
   Size:       1163     598
 
AccI GT'mk_AC

Cuts at:      0     305    1761 
   Size:        305    1456
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     224    1435    1761 
   Size:        224    1211     326

  Fragments arranged by size: 

                1211     326     224
 
AflII C'TTAA_G

Cuts at:      0    1591    1761 
   Size:       1591     170
 
AflIII A'CryG_T

Cuts at:      0     779    1716    1761 
   Size:        779     937      45

  Fragments arranged by size: 

                 937     779      45
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     925     957    1761 
   Size:        925      32     804

  Fragments arranged by size: 

                 925     804      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     879    1761 
   Size:        879     882
 
ApoI r'AATT_y

Cuts at:      0     536    1761 
   Size:        536    1225
 
AvaI C'yCGr_G

Cuts at:      0     310     515     809    1761 
   Size:        310     205     294     952

  Fragments arranged by size: 

                 952     310     294     205
 
BamHI G'GATC_C

Cuts at:      0    1064    1761 
   Size:       1064     697
 
BanII G_rGCy'C

Cuts at:      0     153    1544    1643    1656    1761 
   Size:        153    1391      99      13     105

  Fragments arranged by size: 

                1391     153     105      99      13
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     230     257     502    1399    1761 
   Size:        230      27     245     897     362

  Fragments arranged by size: 

                 897     362     245     230      27
 
BbvCI CC'TCA_GC

Cuts at:      0      79     216    1270    1689    1761 
   Size:         79     137    1054     419      72

  Fragments arranged by size: 

                1054     419     137      79      72
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     685     719    1267    1301    1761 
   Size:        685      34     548      34     460

  Fragments arranged by size: 

                 685     548     460      34      34
 
BlpI GC'TnA_GC

Cuts at:      0     452    1761 
   Size:        452    1309
 
Bme1580I G_kGCm'C

Cuts at:      0      96    1761 
   Size:         96    1665
 
BmgBI CAC'GTC

Cuts at:      0     743    1320    1761 
   Size:        743     577     441

  Fragments arranged by size: 

                 743     577     441
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     119    1515    1761 
   Size:        119    1396     246

  Fragments arranged by size: 

                1396     246     119
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     153    1026    1761 
   Size:        153     873     735

  Fragments arranged by size: 

                 873     735     153
 
Bpu10I CC'TnA_GC

Cuts at:      0      79     216    1270    1642    1689    1761 
   Size:         79     137    1054     372      47      72

  Fragments arranged by size: 

                1054     372     137      79      72      47
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      41     307     691    1044    1761 
   Size:         41     266     384     353     717

  Fragments arranged by size: 

                 717     384     353     266      41
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     220     250     925     955    1761 
   Size:        220      30     675      30     806

  Fragments arranged by size: 

                 806     675     220      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     212     583     837    1761 
   Size:        212     371     254     924

  Fragments arranged by size: 

                 924     371     254     212
 
BseYI C'CCAG_C

Cuts at:      0    1655    1761 
   Size:       1655     106
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     108     971    1761 
   Size:         49      59     863     790

  Fragments arranged by size: 

                 863     790      59      49
 
BsiHKAI G_wGCw'C

Cuts at:      0     153    1761 
   Size:        153    1608
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1297    1761 
   Size:       1297     464
 
Bsp1286I G_dGCh'C

Cuts at:      0      96     153    1544    1643    1656    1761 
   Size:         96      57    1391      99      13     105

  Fragments arranged by size: 

                1391     105      99      96      57      13
 
BspHI T'CATG_A

Cuts at:      0     269    1122    1761 
   Size:        269     853     639

  Fragments arranged by size: 

                 853     639     269
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     392    1163    1761 
   Size:        392     771     598

  Fragments arranged by size: 

                 771     598     392
 
BsrBI CCG'CTC

Cuts at:      0     512    1761 
   Size:        512    1249
 
BsrDI GCAATG_nn'

Cuts at:      0    1283    1761 
   Size:       1283     478
 
BstYI r'GATC_y

Cuts at:      0    1050    1064    1148    1761 
   Size:       1050      14      84     613

  Fragments arranged by size: 

                1050     613      84      14
 
Bsu36I CC'TnA_GG

Cuts at:      0     224    1761 
   Size:        224    1537
 
BtsI GCAGTG_nn'

Cuts at:      0     959    1761 
   Size:        959     802
 
DraI TTT'AAA

Cuts at:      0     206    1761 
   Size:        206    1555
 
EarI CTCTTCn'nnn_

Cuts at:      0     367    1427    1752    1761 
   Size:        367    1060     325       9

  Fragments arranged by size: 

                1060     367     325       9
 
EcoICRI GAG'CTC

Cuts at:      0     151    1761 
   Size:        151    1610
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     153     224    1026    1435    1761 
   Size:        153      71     802     409     326

  Fragments arranged by size: 

                 802     409     326     153      71
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     403    1761 
   Size:        403    1358
 
EcoO109I rG'GnC_Cy

Cuts at:      0     627    1761 
   Size:        627    1134
 
EcoRI G'AATT_C

Cuts at:      0     536    1761 
   Size:        536    1225
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     910    1027    1235    1617    1761 
   Size:        910     117     208     382     144

  Fragments arranged by size: 

                 910     382     208     144     117
 
HaeII r_GCGC'y

Cuts at:      0     734    1761 
   Size:        734    1027
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     151     183     220     252    1024    1056    1121    1153 
   Size:        151      32      37      32     772      32      65      32

Cuts at:   1153    1706    1738    1761 
   Size:        553      32      23

  Fragments arranged by size: 

                 772     553     151      65      37      32      32      32
                  32      32      23
 
HindIII A'AGCT_T

Cuts at:      0     862    1217    1761 
   Size:        862     355     544

  Fragments arranged by size: 

                 862     544     355
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1166    1761 
   Size:       1166     595
 
MspA1I CmG'CkG

Cuts at:      0    1116    1761 
   Size:       1116     645
 
NspI r_CATG'y

Cuts at:      0     434     783    1720    1761 
   Size:        434     349     937      41

  Fragments arranged by size: 

                 937     434     349      41
 
PasI CC'CwG_GG

Cuts at:      0    1636    1761 
   Size:       1636     125
 
PciI A'CATG_T

Cuts at:      0     779    1716    1761 
   Size:        779     937      45

  Fragments arranged by size: 

                 937     779      45
 
PfoI T'CCnGG_A

Cuts at:      0    1340    1427    1761 
   Size:       1340      87     334

  Fragments arranged by size: 

                1340     334      87
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     925     957    1761 
   Size:        925      32     804

  Fragments arranged by size: 

                 925     804      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     627    1761 
   Size:        627    1134
 
PstI C_TGCA'G

Cuts at:      0    1115    1729    1761 
   Size:       1115     614      32

  Fragments arranged by size: 

                1115     614      32
 
PvuII CAG'CTG

Cuts at:      0    1116    1761 
   Size:       1116     645
 
SacI G_AGCT'C

Cuts at:      0     153    1761 
   Size:        153    1608
 
SfcI C'TryA_G

Cuts at:      0    1111    1377    1725    1761 
   Size:       1111     266     348      36

  Fragments arranged by size: 

                1111     348     266      36
 
SmaI CCC'GGG

Cuts at:      0     517     811    1761 
   Size:        517     294     950

  Fragments arranged by size: 

                 950     517     294
 
SmlI C'TyrA_G

Cuts at:      0     310    1591    1761 
   Size:        310    1281     170

  Fragments arranged by size: 

                1281     310     170
 
StyI C'CwwG_G

Cuts at:      0    1088    1761 
   Size:       1088     673
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     201    1761 
   Size:        201    1560
 
TatI w'GTAC_w

Cuts at:      0    1352    1761 
   Size:       1352     409
 
XbaI T'CTAG_A

Cuts at:      0     846    1761 
   Size:        846     915
 
XhoI C'TCGA_G

Cuts at:      0     310    1761 
   Size:        310    1451
 
XmaI C'CCGG_G

Cuts at:      0     515     809    1761 
   Size:        515     294     952

  Fragments arranged by size: 

                 952     515     294
 
XmnI GAAnn'nnTTC

Cuts at:      0     160    1253    1761 
   Size:        160    1093     508

  Fragments arranged by size: 

                1093     508     160
 

 Enzymes that do cut:

     AarI     AccI     AcuI    AflII   AflIII     AloI    AlwNI     ApoI
     AvaI    BamHI    BanII     BbsI    BbvCI     BcgI     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I     BsaI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BstYI   Bsu36I     BtsI     DraI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI    HaeII    Hin4I  HindIII     MmeI   MspA1I
     NspI     PasI     PciI     PfoI     PpiI    PpuMI     PstI    PvuII
     SacI     SfcI     SmaI     SmlI     StyI    TaqII     TatI     XbaI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI     AlfI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BanI
     BbeI    BciVI     BclI    BfrBI     BglI    BglII     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI    EcoRV     FseI     FspI    FspAI   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI    PflMI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XcmI
     ZraI
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