Restriction map for pF1KB8655
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BamHI       BanI        BbeI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaBI       BsaHI       BseAI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
BstBI       BstEII      BstPI       Bsu36I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII        1069  CGCG                 CG^CG
Asp700I       199  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         835  GTGCTC               G[AT]GC[AT]^C
AvaI         1159  CCCGAG               C^[CT]CG[GA]G
BalI         1115  TGGCCA               TGG^CCA
BanII        1155  GAGCCC               G[GA]GC[CT]^C
BbrPI         130  CACGTG               CAC^GTG
BbvCI        1651  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1304  GCACCAACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1382  CTAG                 C^TAG
BlpI         1404  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1404  GCTCAGC              GC^T[ACGT]AGC
BsaI         1534  GAGACC               GGTCTC(1/5)
BsaWI         761  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       835  GTGCTC               G[AT]GC[AT]^C
BsoBI        1159  CCCGAG               C^[CT]CG[GA]G
BsrDI        1287  CATTGC               GCAATG(2/0)
Bst1107I      325  GTATAC               GTA^TAC
BstAPI        498  GCAAACCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1069  CGCG                 CG^CG
BstXI        1118  CCAGCGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       325  GTATAC               GTA^TAC
CelII        1404  GCTCAGC              GC^T[ACGT]AGC
EaeI         1115  TGGCCA               [CT]^GGCC[GA]
EcoNI         963  CCTGAAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI         1675  GACGC                GACGC(5/10)
HinfI        1036  GAGTC                G^A[ACGT]TC
MaeI         1382  CTAG                 C^TAG
MluNI        1115  TGGCCA               TGG^CCA
MscI         1115  TGGCCA               TGG^CCA
MseI          356  TTAA                 T^TAA
MvnI         1069  CGCG                 CG^CG
NaeI         1386  GCCGGC               GCC^GGC
NgoMIV       1386  GCCGGC               G^CCGGC
PleI         1036  GAGTC                GAGTC(4/5)
PmaCI         130  CACGTG               CAC^GTG
PmlI          130  CACGTG               CAC^GTG
PshAI         949  GACATGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          733  CTGCAG               CTGCA^G
SapI          421  GAAGAGC              GCTCTTC(1/4)
SfcI          733  CTGCAG               C^T[GA][CT]AG
StuI          110  AGGCCT               AGG^CCT
TaqI         1438  TCGA                 T^CGA
Tru9I         356  TTAA                 T^TAA
TthHB8I      1438  TCGA                 T^CGA
XmnI          199  GAACAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          241  GTAGAC               GT^[AC][GT]AC
              325  GTATAC              
AcsI         1014  GAATTT               [GA]^AATT[CT]
             1048  AAATTT              
AfaI         1236  GTAC                 GT^AC
             1338  GTAC                
AlwNI         152  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1195  CAGGCTCTG           
ApoI         1014  GAATTT               [GA]^AATT[CT]
             1048  AAATTT              
AspI          585  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1123  GACGTGGTC           
BcnI          536  CCCGG                CC^[GC]GG
             1604  CCCGG               
BsaAI         130  CACGTG               [CT]AC^GT[GA]
              712  TACGTG              
BsmAI         545  GAGAC                GTCTC(1/5)
             1534  GAGAC               
BsmI          426  GCATTC               GAATGC(1/-1)
             1085  GCATTC              
BspMI        1415  ACCTGC               ACCTGC(4/8)
              969  GCAGGT              
BsrFI        1386  GCCGGC               [GA]^CCGG[CT]
             1448  ACCGGC              
BstYI         101  AGATCC               [GA]^GATC[CT]
              806  GGATCT              
Cfr10I       1386  GCCGGC               [GA]^CCGG[CT]
             1448  ACCGGC              
HaeII         737  AGCGCC               [GA]GCGC^[CT]
             1451  GGCGCT              
MflI          101  AGATCC               [GA]^GATC[CT]
              806  GGATCT              
NciI          536  CCCGG                CC^[GC]GG
             1604  CCCGG               
NspI          644  ACATGC               [GA]CATG^[CT]
             1064  ACATGC              
PflFI         585  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1123  GACGTGGTC           
PpuMI          53  GGGTCCT              [GA]G^G[AT]CC[CT]
              632  AGGACCT             
PvuII        1264  CAGCTG               CAG^CTG
             1293  CAGCTG              
RsaI         1236  GTAC                 GT^AC
             1338  GTAC                
SmlI          187  CTTGAG               C^T[CT][GA]AG
             1420  CTCAAG              
Tth111I       585  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1123  GACGTGGTC           
XhoII         101  AGATCC               [GA]^GATC[CT]
              806  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbsI        
BbvI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NcoI        NdeII       NlaIII      
NlaIV       PflMI       Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI        
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