Restriction map for pF1KB6741
 (Circular) (Six-base) MAPSORT of: pF1KB6741.seq  Check: 9157  from: 1  to: 3407



pF1KB6741  3407 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    266     266 
   Size:       3407
 
AccI GT'mk_AC

Cuts at:    445     445 
   Size:       3407
 
Acc65I G'GTAC_C

Cuts at:    423     423 
   Size:       3407
 
AclI AA'CG_TT

Cuts at:   3247    3247 
   Size:       3407
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     285     415    1232    1664    2411    2679     271 
   Size:         14     130     817     432     747     268     999

  Fragments arranged by size: 

                 999     817     747     432     268     130      14
 
AflIII A'CryG_T

Cuts at:    759    1863    2840     759 
   Size:       1104     977    1326

  Fragments arranged by size: 

                1326    1104     977
 
AgeI A'CCGG_T

Cuts at:   1795    1795 
   Size:       3407
 
AlwNI CAG_nnn'CTG

Cuts at:    249     712    2279     249 
   Size:        463    1567    1377

  Fragments arranged by size: 

                1567    1377     463
 
ApaLI G'TGCA_C

Cuts at:   2177    2177 
   Size:       3407
 
ApoI r'AATT_y

Cuts at:    411    2760    3016     411 
   Size:       2349     256     802

  Fragments arranged by size: 

                2349     802     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3407
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3407
 
AvaI C'yCGr_G

Cuts at:    427     427 
   Size:       3407
 
BamHI G'GATC_C

Cuts at:    432     432 
   Size:       3407
 
BanI G'GyrC_C

Cuts at:    423     837    1085    1120     423 
   Size:        414     248      35    2710

  Fragments arranged by size: 

                2710     414     248      35
 
BanII G_rGCy'C

Cuts at:    421    1451     421 
   Size:       1030    2377
 
BbeI G_GCGC'C

Cuts at:    841    1089     841 
   Size:        248    3159
 
BbsI GAAGACnn'nnnn_

Cuts at:    403     403 
   Size:       3407
 
BciVI GTATCCnnnnn_n'

Cuts at:   1305    2066    3407    1305 
   Size:        761    1341    1305

  Fragments arranged by size: 

                1341    1305     761
 
BclI T'GATC_A

Cuts at:    263     927     263 
   Size:        664    2743
 
BglII A'GATC_T

Cuts at:    233     323     922    2959     233 
   Size:         90     599    2037     681

  Fragments arranged by size: 

                2037     681     599      90
 
BlpI GC'TnA_GC

Cuts at:    176     519     176 
   Size:        343    3064
 
Bme1580I G_kGCm'C

Cuts at:   1032    1125    2181    1032 
   Size:         93    1056    2258

  Fragments arranged by size: 

                2258    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    322     619     700    1035    3179     322 
   Size:        297      81     335    2144     550

  Fragments arranged by size: 

                2144     550     335     297      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1774    1774 
   Size:       3407
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    583    1462    1954    2252     583 
   Size:        879     492     298    1738

  Fragments arranged by size: 

                1738     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3111    3111 
   Size:       3407
 
BsaAI yAC'GTr

Cuts at:    760     760 
   Size:       3407
 
BsaBI GATnn'nnATC

Cuts at:      6     921    2958       6 
   Size:        915    2037     455

  Fragments arranged by size: 

                2037     915     455
 
BsaHI Gr'CG_yC

Cuts at:    838    1086    1788    3284     838 
   Size:        248     702    1496     961

  Fragments arranged by size: 

                1496     961     702     248
 
BsaWI w'CCGG_w

Cuts at:    596    1117    1795    2069    2216    2627     596 
   Size:        521     678     274     147     411    1376

  Fragments arranged by size: 

                1376     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    832    1590    2167    2663     832 
   Size:        758     577     496    1576

  Fragments arranged by size: 

                1576     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     949     995    2203     104 
   Size:        845      46    1208    1308

  Fragments arranged by size: 

                1308    1208     845      46
 
BsiHKAI G_wGCw'C

Cuts at:    421    1199    1389    2181     421 
   Size:        778     190     792    1647

  Fragments arranged by size: 

                1647     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    421    1032    1125    1199    1389    1451    2181     421 
   Size:        611      93      74     190      62     730    1647

  Fragments arranged by size: 

                1647     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   2627    2627 
   Size:       3407
 
BspHI T'CATG_A

Cuts at:    143    2583     143 
   Size:       2440     967
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    266     457     671     973    1354     266 
   Size:        191     214     302     381    2319

  Fragments arranged by size: 

                2319     381     302     214     191
 
BsrBI CCG'CTC

Cuts at:     50    1699    1753    3237    3402      50 
   Size:       1649      54    1484     165      55

  Fragments arranged by size: 

                1649    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1319    1319 
   Size:       3407
 
BsrFI r'CCGG_y

Cuts at:   1405    1586    1795    1405 
   Size:        181     209    3017

  Fragments arranged by size: 

                3017     209     181
 
BssSI C'ACGA_G

Cuts at:   1678    2036    1678 
   Size:        358    3049
 
BstAPI GCAn_nnn'nTGC

Cuts at:    784     784 
   Size:       3407
 
BstBI TT'CG_AA

Cuts at:   1768    1768 
   Size:       3407
 
BstYI r'GATC_y

Cuts at:      1     233     323     432     899     922    1257    1503    2515 
   Size:        232      90     109     467      23     335     246    1012

Cuts at:   2515    2601    2613    2959       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     246     232     109
                  90      86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1357    1538    1701    1357 
   Size:        181     163    3063

  Fragments arranged by size: 

                3063     181     163
 
BtsI GCAGTG_nn'

Cuts at:    128     650     128 
   Size:        522    2885
 
DraI TTT'AAA

Cuts at:    406     406 
   Size:       3407
 
DrdI GACnn_nn'nnGTC

Cuts at:   1113    1971    1113 
   Size:        858    2549
 
EaeI y'GGCC_r

Cuts at:    992    1166    1557    1584    2929     992 
   Size:        174     391      27    1345    1470

  Fragments arranged by size: 

                1470    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    992     992 
   Size:       3407
 
EarI CTCTTCn'nnn_

Cuts at:    247    1430    1640     247 
   Size:       1183     210    2014

  Fragments arranged by size: 

                2014    1183     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1615    1925    2071    3217    1615 
   Size:        310     146    1146    1805

  Fragments arranged by size: 

                1805    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    419     419 
   Size:       3407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     285     415    1232    1664    2411    2679     271 
   Size:         14     130     817     432     747     268     999

  Fragments arranged by size: 

                 999     817     747     432     268     130      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:    243     243 
   Size:       3407
 
EcoO109I rG'GnC_Cy

Cuts at:    546     546 
   Size:       3407
 
EcoRI G'AATT_C

Cuts at:    411     411 
   Size:       3407
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    255    1426     255 
   Size:       1171    2236
 
FspI TGC'GCA

Cuts at:   1188    1188 
   Size:       3407
 
HaeII r_GCGC'y

Cuts at:    841    1089    2111    3090     841 
   Size:        248    1022     979    1158

  Fragments arranged by size: 

                1158    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1249    1279    1281    1311    1249 
   Size:         30       2      30    3345

  Fragments arranged by size: 

                3345      30      30       2
 
HincII GTy'rAC

Cuts at:    446     446 
   Size:       3407
 
KasI G'GCGC_C

Cuts at:    837    1085     837 
   Size:        248    3159
 
KpnI G_GTAC'C

Cuts at:    427     427 
   Size:       3407
 
MluI A'CGCG_T

Cuts at:   2840    2840 
   Size:       3407
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2078    2262    2078 
   Size:        184    3223
 
MscI TGG'CCA

Cuts at:   1168    1168 
   Size:       3407
 
MslI CAynn'nnrTG

Cuts at:   1396    1523    1396 
   Size:        127    3280
 
MspA1I CmG'CkG

Cuts at:    518     832    1192    2205    2450     518 
   Size:        314     360    1013     245    1475

  Fragments arranged by size: 

                1475    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1588    1588 
   Size:       3407
 
NarI GG'CG_CC

Cuts at:    838    1086     838 
   Size:        248    3159
 
NgoMIV G'CCGG_C

Cuts at:   1586    1586 
   Size:       3407
 
NspI r_CATG'y

Cuts at:    460     960    1867     460 
   Size:        500     907    2000

  Fragments arranged by size: 

                2000     907     500
 
PasI CC'CwG_GG

Cuts at:    165     165 
   Size:       3407
 
PciI A'CATG_T

Cuts at:   1863    1863 
   Size:       3407
 
PmeI GTTT'AAAC

Cuts at:    406     406 
   Size:       3407
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2546    2578    2546 
   Size:         32    3375
 
PstI C_TGCA'G

Cuts at:    123     454    1139     123 
   Size:        331     685    2391

  Fragments arranged by size: 

                2391     685     331
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3407
 
PvuII CAG'CTG

Cuts at:    832    1192     832 
   Size:        360    3047
 
RsrII CG'GwC_CG

Cuts at:   1602    1602 
   Size:       3407
 
SacI G_AGCT'C

Cuts at:    421     421 
   Size:       3407
 
SalI G'TCGA_C

Cuts at:    444     444 
   Size:       3407
 
SapI GCTCTTCn'nnn_

Cuts at:    247    1430    1640     247 
   Size:       1183     210    2014

  Fragments arranged by size: 

                2014    1183     210
 
SbfI CC_TGCA'GG

Cuts at:    454     454 
   Size:       3407
 
ScaI AGT'ACT

Cuts at:   2924    2924 
   Size:       3407
 
SfcI C'TryA_G

Cuts at:     33     119     450    1135    2128    2319      33 
   Size:         86     331     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     331     191      86
 
SfoI GGC'GCC

Cuts at:    839    1087     839 
   Size:        248    3159
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3407
 
SmaI CCC'GGG

Cuts at:    429     429 
   Size:       3407
 
SmlI C'TyrA_G

Cuts at:    562    1477    1969    2231     562 
   Size:        915     492     262    1738

  Fragments arranged by size: 

                1738     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3407
 
SphI G_CATG'C

Cuts at:    460     960     460 
   Size:        500    2907
 
StyI C'CwwG_G

Cuts at:    541     895     541 
   Size:        354    3053
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    990    1123    1582    1791     990 
   Size:        133     459     209    2606

  Fragments arranged by size: 

                2606     459     209     133
 
TatI w'GTAC_w

Cuts at:   2922    2922 
   Size:       3407
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    179     211    2924    2956     179 
   Size:         32    2713      32     630

  Fragments arranged by size: 

                2713     630      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1204    1204 
   Size:       3407
 
XbaI T'CTAG_A

Cuts at:    438     438 
   Size:       3407
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    344     902     344 
   Size:        558    2849
 
XmaI C'CCGG_G

Cuts at:    427     427 
   Size:       3407
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PasI     PciI     PmeI     PpiI     PstI     PvuI
    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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