Restriction map for pF1KB6447
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AflII       AflIII      AhdI        Alw44I      AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BseRI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstZ17I     Bsu36I      Cfr13I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     FauI        FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KasI        KpnI        KspI        MaeII       
MamI        MfeI        MluI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccII         316  CGCG                 CG^CG
AccIII        719  TCCGGA               T^CCGGA
AcyI          105  GGCGTC               G[GA]^CG[CT]C
AgeI          671  ACCGGT               A^CCGGT
Asp700I       570  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         412  GAGCAC               G[AT]GC[AT]^C
BalI          560  TGGCCA               TGG^CCA
BamHI         311  GGATCC               G^GATCC
BanI          547  GGCACC               G^G[CT][GA]CC
BfaI          466  CTAG                 C^TAG
BlpI          423  GCTGAGC              GC^T[ACGT]AGC
BmyI          412  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      423  GCTGAGC              GC^T[ACGT]AGC
BsaHI         105  GGCGTC               G[GA]^CG[CT]C
BsaJI         488  CCGAGG               C^C[ACGT][ACGT]GG
BseAI         719  TCCGGA               T^CCGGA
BsgI          175  GTGCAG               GTGCAG(16/14)
BsiEI         673  CGGTCG               CG[GA][CT]^CG
BsiHKAI       412  GAGCAC               G[AT]GC[AT]^C
BsmAI         148  GAGAC                GTCTC(1/5)
BsmFI         114  GTCCC                GGGAC(10/14)
Bsp1286I      412  GAGCAC               G[AGT]GC[ACT]^C
BspEI         719  TCCGGA               T^CCGGA
BspMI         218  ACCTGC               ACCTGC(4/8)
BsrBI          61  GAGCGG               CCGCTC(-3/-3)
BsrFI         671  ACCGGT               [GA]^CCGG[CT]
BstUI         316  CGCG                 CG^CG
BstYI         311  GGATCC               [GA]^GATC[CT]
CelII         423  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        671  ACCGGT               [GA]^CCGG[CT]
EcoT22I       160  ATGCAT               ATGCA^T
HgaI          106  GCGTC                GACGC(5/10)
Hin1I         105  GGCGTC               G[GA]^CG[CT]C
MaeI          466  CTAG                 C^TAG
MaeIII        607  GTGAC                ^GT[ACGT]AC
MflI          311  GGATCC               [GA]^GATC[CT]
MluNI         560  TGGCCA               TGG^CCA
MroI          719  TCCGGA               T^CCGGA
MscI          560  TGGCCA               TGG^CCA
MslI          552  CATTAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          316  CGCG                 CG^CG
NsiI          160  ATGCAT               ATGCA^T
NspI          362  ACATGC               [GA]CATG^[CT]
PinAI         671  ACCGGT               A^CCGGT
PvuII         640  CAGCTG               CAG^CTG
ScaI           38  AGTACT               AGT^ACT
SmlI          349  CTCAAG               C^T[CT][GA]AG
TfiI          493  GAATC                G^A[AT]TC
Tsp45I        607  GTGAC                ^GT[GC]AC
Tsp509I       659  AATT                 ^AATT
TspRI         620  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI          122  CCATCTGGCTACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         311  GGATCC               [GA]^GATC[CT]
XmnI          570  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI           39  GTAC                 GT^AC
              462  GTAC                
AlwNI         367  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              418  CAGCGGCTG           
BbsI           25  GAAGAC               GAAGAC(2/6)
              531  GAAGAC              
BpuAI          25  GAAGAC               GAAGAC(2/6)
              531  GAAGAC              
BsaWI         671  ACCGGT               [AT]^CCGG[AT]
              719  TCCGGA              
BssKI          30  CCTGG                ^CC[ACGT]GG
              291  CCAGG               
BstNI          30  CCTGG                CC^[AT]GG
              291  CCAGG               
BstXI         118  CCATCCATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              551  CCATTAAGATGG        
EaeI           32  TGGCCG               [CT]^GGCC[GA]
              560  TGGCCA              
EarI          574  CTCTTC               CTCTTC(1/4)
               19  GAAGAG              
EcoNI         465  CCTAGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              597  CCTGAAGGAGG         
EcoRII         30  CCTGG                ^CC[AT]GG
              291  CCAGG               
HaeII         399  GGCGCT               [GA]GCGC^[CT]
              427  AGCGCC              
HaeIII         33  GGCC                 GG^CC
              561  GGCC                
HphI          606  GGTGA                GGTGA(8/7)
              693  GGTGA               
Ksp632I       574  CTCTTC               CTCTTC(1/4)
               19  GAAGAG              
MseI          242  TTAA                 T^TAA
              554  TTAA                
MspA1I        418  CAGCGG               C[AC]G^C[GT]G
              640  CAGCTG              
MvaI           30  CCTGG                CC^[AT]GG
              291  CCAGG               
NlaIV         311  GGATCC               GG[ACGT]^[ACGT]CC
              547  GGCACC              
PstI           49  CTGCAG               CTGCA^G
               70  CTGCAG              
RsaI           39  GTAC                 GT^AC
              462  GTAC                
ScrFI          30  CCTGG                CC^[ACGT]GG
              291  CCAGG               
SfcI           49  CTGCAG               C^T[GA][CT]AG
               70  CTGCAG              
Tru9I         242  TTAA                 T^TAA
              554  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AciI        AluI        AlwI        BbvI        BpmI        
BsiYI       BslI        BsrI        BstF5I      Cac8I       
CfoI        DdeI        DpnII       Fnu4HI      FokI        
HapII       HhaI        HinP1I      HinfI       HpaII       
ItaI        MboI        MboII       MnlI        MspI        
MwoI        NdeII       NlaIII      PleI        Sau3AI      
SfaNI       TseI        
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