Restriction map for pF1KB6352
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BbrPI       
BbvCI       BcgI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmI        Bsp1407I    BspEI       BspHI       BspLU11I    
BsrGI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       CpoI        
DraI        DraII       DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        HgaI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MseI        MslI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

AcyI          620  GGCGCC               G[GA]^CG[CT]C
AlwNI         209  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI          46  GAGCTC               G[AT]GC[AT]^C
AvaI          491  CCCGGG               C^[CT]CG[GA]G
AviII         341  TGCGCA               TGC^GCA
BalI          464  TGGCCA               TGG^CCA
BamHI         524  GGATCC               G^GATCC
BanI          620  GGCGCC               G^G[CT][GA]CC
BanII          46  GAGCTC               G[GA]GC[CT]^C
BbeI          620  GGCGCC               GGCGC^C
BciVI         711  GTATCC               GTATCC(6/5)
BclI           59  TGATCA               T^GATCA
BmyI           46  GAGCTC               G[AGT]GC[ACT]^C
BpmI          473  CTGGAG               CTGGAG(16/14)
BsaHI         620  GGCGCC               G[GA]^CG[CT]C
BsaI          304  GGTCTC               GGTCTC(1/5)
BsaWI         164  ACCGGA               [AT]^CCGG[AT]
BsiHKAI        46  GAGCTC               G[AT]GC[AT]^C
BsmBI         661  GAGACG               CGTCTC(1/5)
BsmFI         512  GGGAC                GGGAC(10/14)
BsoBI         491  CCCGGG               C^[CT]CG[GA]G
Bsp1286I       46  GAGCTC               G[AGT]GC[ACT]^C
BspDI         583  ATCGAT               AT^CGAT
BspMI         724  ACCTGC               ACCTGC(4/8)
BsrBI         127  GAGCGG               CCGCTC(-3/-3)
BsrDI          27  CATTGC               GCAATG(2/0)
BsrI          642  CCAGT                ACTGG(1/-1)
BstAPI        693  GCAGCTCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         524  GGATCC               [GA]^GATC[CT]
ClaI          583  ATCGAT               AT^CGAT
DrdI          775  GACTACTCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          503  CCGTGG               C^C[GA][CT]GG
EcoT14I       197  CCAAGG               C^C[AT][AT]GG
FbaI           59  TGATCA               T^GATCA
FspI          341  TGCGCA               TGC^GCA
HaeII         620  GGCGCC               [GA]GCGC^[CT]
Hin1I         620  GGCGCC               G[GA]^CG[CT]C
KasI          620  GGCGCC               G^GCGCC
MflI          524  GGATCC               [GA]^GATC[CT]
MluNI         464  TGGCCA               TGG^CCA
MscI          464  TGGCCA               TGG^CCA
NaeI          617  GCCGGC               GCC^GGC
NarI          620  GGCGCC               GG^CGCC
NgoMIV        617  GCCGGC               G^CCGGC
PleI          316  GACTC                GAGTC(4/5)
PstI          367  CTGCAG               CTGCA^G
PvuII          55  CAGCTG               CAG^CTG
SacI           46  GAGCTC               GAGCT^C
SapI           76  GAAGAGC              GCTCTTC(1/4)
SfcI          367  CTGCAG               C^T[GA][CT]AG
SfoI          620  GGCGCC               GGC^GCC
SmaI          491  CCCGGG               CCC^GGG
StuI          674  AGGCCT               AGG^CCT
StyI          197  CCAAGG               C^C[AT][AT]GG
TaqI          584  TCGA                 T^CGA
TfiI           19  GATTC                G^A[AT]TC
Tsp45I        413  GTGAC                ^GT[GC]AC
TthHB8I       584  TCGA                 T^CGA
XhoII         524  GGATCC               [GA]^GATC[CT]
XmaCI         491  CCCGGG               C^CCGGG
XmaI          491  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AocI          449  CCTCAGG              CC^T[ACGT]AGG
              670  CCTGAGG             
AvaII         666  GGACC                G^G[AT]CC
              783  GGTCC               
BsaAI         460  TACGTG               [CT]AC^GT[GA]
              847  TACGTG              
BsrFI         301  GCCGGT               [GA]^CCGG[CT]
              617  GCCGGC              
BssSI           7  CTCGTG               CACGAG(-5/-1)
              697  CTCGTG              
Bsu36I        449  CCTCAGG              CC^T[ACGT]AGG
              670  CCTGAGG             
Cac8I         617  GCCGGC               GC[ACGT]^[ACGT]GC
              702  GCTGGC              
Cfr10I        301  GCCGGT               [GA]^CCGG[CT]
              617  GCCGGC              
EarI           76  GAAGAG               CTCTTC(1/4)
              274  GAAGAG              
Eco81I        449  CCTCAGG              CC^T[ACGT]AGG
              670  CCTGAGG             
EcoNI         445  CCTGCCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              528  CCTGGCCGAGG         
FauI           23  CCCGC                CCCGC(4/6)
              633  CCCGC               
HinfI          19  GATTC                G^A[ACGT]TC
              316  GACTC               
HphI          412  GGTGA                GGTGA(8/7)
              495  GGTGA               
Ksp632I        76  GAAGAG               CTCTTC(1/4)
              274  GAAGAG              
MaeII         461  ACGT                 A^CGT
              848  ACGT                
MaeIII        413  GTGAC                ^GT[ACGT]AC
              545  GTTAC               
SfaNI         132  GATGC                GCATC(5/9)
              205  GATGC               
TspRI         520  CAGTG                CA[GC]TG[ACGT][ACGT]^
              643  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   46 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        BbsI        
BbvI        BcnI        BpuAI       BsaJI       BsiYI       
BslI        BsmAI       BssKI       BstF5I      BstNI       
BstUI       CfoI        Cfr13I      DdeI        DpnII       
EaeI        EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TseI        
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