Restriction map for pF1KE0219
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BanI        BanII       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiHKAI     BsiWI       BsmFI       BsmI        Bsp1286I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       CfoI        ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MslI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TfiI        Tth111I     Van91I      XbaI        
XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII          99  CGCG                 CG^CG
AcsI          302  GAATTC               [GA]^AATT[CT]
ApoI          302  GAATTC               [GA]^AATT[CT]
BalI          275  TGGCCA               TGG^CCA
BamHI         120  GGATCC               G^GATCC
BbvCI         318  CCTCAGC              CCTCAGC(-5/-2)
BbvI          215  GCAGC                GCAGC(8/12)
BcnI          117  CCGGG                CC^[GC]GG
BpmI            7  CTGGAG               CTGGAG(16/14)
BsaAI         244  TACGTA               [CT]AC^GT[GA]
BsaJI         284  CCCTGG               C^C[ACGT][ACGT]GG
BsiYI         111  CCAGGACCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          111  CCAGGACCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          10  GAGAC                GTCTC(1/5)
BsmBI          10  GAGACG               CGTCTC(1/5)
Bsp1407I      251  TGTACA               T^GTACA
BspMI         361  ACCTGC               ACCTGC(4/8)
BsrGI         251  TGTACA               T^GTACA
BstF5I        162  GGATG                GGATG(2/0)
BstUI          99  CGCG                 CG^CG
BstXI         278  CCATTCCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         467  GCCTGC               GC[ACGT]^[ACGT]GC
EaeI          275  TGGCCA               [CT]^GGCC[GA]
EcoRI         302  GAATTC               G^AATTC
FauI          298  GCGGG                CCCGC(4/6)
FokI          162  GGATG                GGATG(9/13)
HphI          180  GGTGA                GGTGA(8/7)
MluNI         275  TGGCCA               TGG^CCA
MscI          275  TGGCCA               TGG^CCA
MseI          343  TTAA                 T^TAA
MvnI           99  CGCG                 CG^CG
NciI          117  CCGGG                CC^[GC]GG
NlaIV         120  GGATCC               GG[ACGT]^[ACGT]CC
PaeR7I         73  CTCGAG               C^TCGAG
PstI          213  CTGCAG               CTGCA^G
PvuII         321  CAGCTG               CAG^CTG
SapI           33  GCTCTTC              GCTCTTC(1/4)
SnaBI         244  TACGTA               TAC^GTA
SspBI         251  TGTACA               T^GTACA
Tru9I         343  TTAA                 T^TAA
TseI          215  GCAGC                G^C[AT]GC
Tsp509I       303  AATT                 ^AATT
TspRI         383  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoI           73  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AvaI           73  CTCGAG               C^[CT]CG[GA]G
               88  CCCGAG              
AvaII         108  GGACC                G^G[AT]CC
              114  GGACC               
BsgI          328  CTGCAC               GTGCAG(16/14)
              385  GTGCAG              
BsoBI          73  CTCGAG               C^[CT]CG[GA]G
               88  CCCGAG              
BsrDI         294  CATTGC               GCAATG(2/0)
              420  CATTGC              
BsrFI         128  ACCGGC               [GA]^CCGG[CT]
              177  GCCGGT              
BstYI         120  GGATCC               [GA]^GATC[CT]
              209  GGATCT              
Cfr10I        128  ACCGGC               [GA]^CCGG[CT]
              177  GCCGGT              
Cfr13I        108  GGACC                G^G[ACGT]CC
              114  GGACC               
DdeI          319  CTCAG                C^T[ACGT]AG
              475  CTGAG               
Fnu4HI         68  GCGGC                GC^[ACGT]GC
              215  GCAGC               
HinfI          49  GACTC                G^A[ACGT]TC
              103  GACTC               
ItaI           68  GCGGC                GC^[ACGT]GC
              215  GCAGC               
MaeII          26  ACGT                 A^CGT
              245  ACGT                
MaeIII        181  GTGAC                ^GT[ACGT]AC
              406  GTGAC               
MflI          120  GGATCC               [GA]^GATC[CT]
              209  GGATCT              
MspA1I         66  CAGCGG               C[AC]G^C[GT]G
              321  CAGCTG              
NlaIII         39  CATG                 CATG^
              220  CATG                
PleI           49  GACTC                GAGTC(4/5)
              103  GACTC               
Sau96I        108  GGACC                G^G[ACGT]CC
              114  GGACC               
SfaNI         449  GATGC                GCATC(5/9)
              141  GCATC               
SfcI           54  CTACAG               C^T[GA][CT]AG
              213  CTGCAG              
SmlI           73  CTCGAG               C^T[CT][GA]AG
              145  CTCAAG              
TaqI           74  TCGA                 T^CGA
              158  TCGA                
Tsp45I        181  GTGAC                ^GT[GC]AC
              406  GTGAC               
TthHB8I        74  TCGA                 T^CGA
              158  TCGA                
XhoII         120  GGATCC               [GA]^GATC[CT]
              209  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BssKI       
BstNI       DpnII       EarI        EcoRII      HaeIII      
HapII       HpaII       Ksp632I     MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
RsaI        Sau3AI      ScrFI       
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