Restriction map for pF1KA1121
 (Linear) (Six-base) MAPSORT of: KIAA1121.seq  Check: 1427  from: 1  to: 4059



KIAA1121.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2924    4059 
   Size:       2924    1135
 
AatII G_ACGT'C

Cuts at:      0     942    4059 
   Size:        942    3117
 
AccI GT'mk_AC

Cuts at:      0     942    2412    2792    4059 
   Size:        942    1470     380    1267

  Fragments arranged by size: 

                1470    1267     942     380
 
AclI AA'CG_TT

Cuts at:      0    2331    4059 
   Size:       2331    1728
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     583    1048    2438    2662    3205    3272    3307    4059 
   Size:        583     465    1390     224     543      67      35     752

  Fragments arranged by size: 

                1390     752     583     543     465     224      67      35
            
 
AflIII A'CryG_T

Cuts at:      0    1010    1655    2709    4059 
   Size:       1010     645    1054    1350

  Fragments arranged by size: 

                1350    1054    1010     645
 
AgeI A'CCGG_T

Cuts at:      0     473    4059 
   Size:        473    3586
 
AhdI GACnn_n'nnGTC

Cuts at:      0      12    4059 
   Size:         12    4047
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      82    4059 
   Size:         82    3977
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     851     883    1150    1182    4059 
   Size:        851      32     267      32    2877

  Fragments arranged by size: 

                2877     851     267      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     456    2859    4059 
   Size:        456    2403    1200

  Fragments arranged by size: 

                2403    1200     456
 
ApoI r'AATT_y

Cuts at:      0     718     976    1999    2902    4059 
   Size:        718     258    1023     903    1157

  Fragments arranged by size: 

                1157    1023     903     718     258
 
AvaI C'yCGr_G

Cuts at:      0     620    3673    4059 
   Size:        620    3053     386

  Fragments arranged by size: 

                3053     620     386
 
BanI G'GyrC_C

Cuts at:      0     154     160    1330    2221    3138    3184    4059 
   Size:        154       6    1170     891     917      46     875

  Fragments arranged by size: 

                1170     917     891     875     154      46       6
 
BanII G_rGCy'C

Cuts at:      0     124     706    1980    4059 
   Size:        124     582    1274    2079

  Fragments arranged by size: 

                2079    1274     582     124
 
BbeI G_GCGC'C

Cuts at:      0     158     164    2225    4059 
   Size:        158       6    2061    1834

  Fragments arranged by size: 

                2061    1834     158       6
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     621    4049    4058    4059 
   Size:        621    3428       9       1

  Fragments arranged by size: 

                3428     621       9       1
 
BbvCI CC'TCA_GC

Cuts at:      0     682    1937    4059 
   Size:        682    1255    2122

  Fragments arranged by size: 

                2122    1255     682
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2165    2199    4059 
   Size:       2165      34    1860

  Fragments arranged by size: 

                2165    1860      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1002    1086    4059 
   Size:       1002      84    2973

  Fragments arranged by size: 

                2973    1002      84
 
BclI T'GATC_A

Cuts at:      0    1299    3774    4059 
   Size:       1299    2475     285

  Fragments arranged by size: 

                2475    1299     285
 
BglI GCCn_nnn'nGGC

Cuts at:      0     251    4059 
   Size:        251    3808
 
BglII A'GATC_T

Cuts at:      0    1518    3195    4059 
   Size:       1518    1677     864

  Fragments arranged by size: 

                1677    1518     864
 
BlpI GC'TnA_GC

Cuts at:      0     700    4059 
   Size:        700    3359
 
Bme1580I G_kGCm'C

Cuts at:      0    1333    1892    4059 
   Size:       1333     559    2167

  Fragments arranged by size: 

                2167    1333     559
 
BmgBI CAC'GTC

Cuts at:      0    3684    4059 
   Size:       3684     375
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2476    4059 
   Size:       2476    1583
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252    1084    2807    4059 
   Size:        252     832    1723    1252

  Fragments arranged by size: 

                1723    1252     832     252
 
Bpu10I CC'TnA_GC

Cuts at:      0     682    1937    4059 
   Size:        682    1255    2122

  Fragments arranged by size: 

                2122    1255     682
 
BsaAI yAC'GTr

Cuts at:      0    3699    4059 
   Size:       3699     360
 
BsaHI Gr'CG_yC

Cuts at:      0     155     161     939    1403    2222    3215    3692    4059 
   Size:        155       6     778     464     819     993     477     367

  Fragments arranged by size: 

                 993     819     778     477     464     367     155       6
            
 
BsaWI w'CCGG_w

Cuts at:      0     473    3824    3953    4059 
   Size:        473    3351     129     106

  Fragments arranged by size: 

                3351     473     129     106
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     158     188     851     881    1015    1045    1150    1180 
   Size:        158      30     663      30     134      30     105      30

Cuts at:   1180    1787    1817    1916    1946    3775    3805    4059 
   Size:        607      30      99      30    1829      30     254

  Fragments arranged by size: 

                1829     663     607     254     158     134     105      99
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61      64     346     349     352    1036    1162    1295 
   Size:         61       3     282       3       3     684     126     133

Cuts at:   1295    2509    2704    3763    4059 
   Size:       1214     195    1059     296

  Fragments arranged by size: 

                1214    1059     684     296     282     195     133     126
                  61       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     103     141     238     274    1745    4059 
   Size:        103      38      97      36    1471    2314

  Fragments arranged by size: 

                2314    1471     103      97      38      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     200     559    1660    1689    3226    3792    4059 
   Size:        200     359    1101      29    1537     566     267

  Fragments arranged by size: 

                1537    1101     566     359     267     200      29
 
BsiEI CG_ry'CG

Cuts at:      0     128     261     573    4059 
   Size:        128     133     312    3486

  Fragments arranged by size: 

                3486     312     133     128
 
BsiHKAI G_wGCw'C

Cuts at:      0      71     706    1399    1980    2696    4059 
   Size:         71     635     693     581     716    1363

  Fragments arranged by size: 

                1363     716     693     635     581      71
 
BsmI GAATG_Cn'

Cuts at:      0    1569    4059 
   Size:       1569    2490
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2761    3967    4059 
   Size:       2761    1206      92

  Fragments arranged by size: 

                2761    1206      92
 
Bsp1286I G_dGCh'C

Cuts at:      0      71     124     706    1333    1399    1892    1980    2696 
   Size:         71      53     582     627      66     493      88     716

Cuts at:   2696    4059 
   Size:       1363

  Fragments arranged by size: 

                1363     716     627     582     493      88      71      66
                  53
 
BspEI T'CCGG_A

Cuts at:      0    3953    4059 
   Size:       3953     106
 
BspHI T'CATG_A

Cuts at:      0     527    4059 
   Size:        527    3532
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1052    1632    2924    3248    4059 
   Size:       1052     580    1292     324     811

  Fragments arranged by size: 

                1292    1052     811     580     324
 
BsrBI CCG'CTC

Cuts at:      0      56     318    3003    4059 
   Size:         56     262    2685    1056

  Fragments arranged by size: 

                2685    1056     262      56
 
BsrDI GCAATG_nn'

Cuts at:      0    2901    4059 
   Size:       2901    1158
 
BsrFI r'CCGG_y

Cuts at:      0     127     157     260     428     473    1071    2609    4059 
   Size:        127      30     103     168      45     598    1538    1450

  Fragments arranged by size: 

                1538    1450     598     168     127     103      45      30
            
 
BsrGI T'GTAC_A

Cuts at:      0    1013    3755    4059 
   Size:       1013    2742     304

  Fragments arranged by size: 

                2742    1013     304
 
BssHII G'CGCG_C

Cuts at:      0      89      91    4059 
   Size:         89       2    3968

  Fragments arranged by size: 

                3968      89       2
 
BstBI TT'CG_AA

Cuts at:      0     857    4059 
   Size:        857    3202
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     420    1002    2281    2831    3657    4059 
   Size:        420     582    1279     550     826     402

  Fragments arranged by size: 

                1279     826     582     550     420     402
 
BstYI r'GATC_y

Cuts at:      0    1518    2955    3195    4059 
   Size:       1518    1437     240     864

  Fragments arranged by size: 

                1518    1437     864     240
 
Bsu36I CC'TnA_GG

Cuts at:      0    1697    4059 
   Size:       1697    2362
 
BtgI C'CryG_G

Cuts at:      0     735     751    1865    2280    4059 
   Size:        735      16    1114     415    1779

  Fragments arranged by size: 

                1779    1114     735     415      16
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     369    4049    4059 
   Size:        369    3680      10

  Fragments arranged by size: 

                3680     369      10
 
BtsI GCAGTG_nn'

Cuts at:      0    1360    4059 
   Size:       1360    2699
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1253    1288    4059 
   Size:       1253      35    2771

  Fragments arranged by size: 

                2771    1253      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2775    4059 
   Size:       2775    1284
 
EaeI y'GGCC_r

Cuts at:      0     125     258     713    1052    1764    2118    3686    4059 
   Size:        125     133     455     339     712     354    1568     373

  Fragments arranged by size: 

                1568     712     455     373     354     339     133     125
            
 
EagI C'GGCC_G

Cuts at:      0     125     258    4059 
   Size:        125     133    3801

  Fragments arranged by size: 

                3801     133     125
 
EarI CTCTTCn'nnn_

Cuts at:      0     302     343     559     643     737    1603    2013    2573 
   Size:        302      41     216      84      94     866     410     560

Cuts at:   2573    3251    4059 
   Size:        678     808

  Fragments arranged by size: 

                 866     808     678     560     410     302     216      94
                  84      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     597    3700    4059 
   Size:        597    3103     359

  Fragments arranged by size: 

                3103     597     359
 
EcoICRI GAG'CTC

Cuts at:      0     704    1978    4059 
   Size:        704    1274    2081

  Fragments arranged by size: 

                2081    1274     704
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     252     583    1048    1084    2438    2662    2807    3205 
   Size:        252     331     465      36    1354     224     145     398

Cuts at:   3205    3272    3307    4059 
   Size:         67      35     752

  Fragments arranged by size: 

                1354     752     465     398     331     252     224     145
                  67      36      35
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     685    4059 
   Size:        685    3374
 
EcoO109I rG'GnC_Cy

Cuts at:      0     747    3237    3912    4059 
   Size:        747    2490     675     147

  Fragments arranged by size: 

                2490     747     675     147
 
FseI GG_CCGG'CC

Cuts at:      0     264    4059 
   Size:        264    3795
 
FspI TGC'GCA

Cuts at:      0     658    4059 
   Size:        658    3401
 
HaeII r_GCGC'y

Cuts at:      0     137     158     164    2225    4059 
   Size:        137      21       6    2061    1834

  Fragments arranged by size: 

                2061    1834     137      21       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30    1015    1047    2992    3024    3163    3195    4059 
   Size:         30     985      32    1945      32     139      32     864

  Fragments arranged by size: 

                1945     985     864     139      32      32      32      30
            
 
HincII GTy'rAC

Cuts at:      0    1041    2413    2544    3099    3818    4059 
   Size:       1041    1372     131     555     719     241

  Fragments arranged by size: 

                1372    1041     719     555     241     131
 
HindIII A'AGCT_T

Cuts at:      0    2632    4059 
   Size:       2632    1427
 
HpaI GTT'AAC

Cuts at:      0    3099    4059 
   Size:       3099     960
 
KasI G'GCGC_C

Cuts at:      0     154     160    2221    4059 
   Size:        154       6    2061    1838

  Fragments arranged by size: 

                2061    1838     154       6
 
MfeI C'AATT_G

Cuts at:      0    1711    2972    4059 
   Size:       1711    1261    1087

  Fragments arranged by size: 

                1711    1261    1087
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1394    2036    4059 
   Size:        293    1101     642    2023

  Fragments arranged by size: 

                2023    1101     642     293
 
MscI TGG'CCA

Cuts at:      0     715    1054    2120    4059 
   Size:        715     339    1066    1939

  Fragments arranged by size: 

                1939    1066     715     339
 
MslI CAynn'nnrTG

Cuts at:      0     418    1138    2279    3144    3463    3799    4021    4059 
   Size:        418     720    1141     865     319     336     222      38

  Fragments arranged by size: 

                1141     865     720     418     336     319     222      38
            
 
MspA1I CmG'CkG

Cuts at:      0     200     212     360     456     753     888    1369    1941 
   Size:        200      12     148      96     297     135     481     572

Cuts at:   1941    2856    4059 
   Size:        915    1203

  Fragments arranged by size: 

                1203     915     572     481     297     200     148     135
                  96      12
 
NaeI GCC'GGC

Cuts at:      0     129     159     262     430    4059 
   Size:        129      30     103     168    3629

  Fragments arranged by size: 

                3629     168     129     103      30
 
NarI GG'CG_CC

Cuts at:      0     155     161    2222    4059 
   Size:        155       6    2061    1837

  Fragments arranged by size: 

                2061    1837     155       6
 
NcoI C'CATG_G

Cuts at:      0    2280    4059 
   Size:       2280    1779
 
NgoMIV G'CCGG_C

Cuts at:      0     127     157     260     428    4059 
   Size:        127      30     103     168    3631

  Fragments arranged by size: 

                3631     168     127     103      30
 
NspI r_CATG'y

Cuts at:      0    1014    1071    2713    2764    4059 
   Size:       1014      57    1642      51    1295

  Fragments arranged by size: 

                1642    1295    1014      57      51
 
PasI CC'CwG_GG

Cuts at:      0    1335    4059 
   Size:       1335    2724
 
PciI A'CATG_T

Cuts at:      0    1010    2709    4059 
   Size:       1010    1699    1350

  Fragments arranged by size: 

                1699    1350    1010
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     510    4059 
   Size:        510    3549
 
PfoI T'CCnGG_A

Cuts at:      0     706    3914    4059 
   Size:        706    3208     145

  Fragments arranged by size: 

                3208     706     145
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     851     883    1150    1182    4059 
   Size:        851      32     267      32    2877

  Fragments arranged by size: 

                2877     851     267      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     747    3237    3912    4059 
   Size:        747    2490     675     147

  Fragments arranged by size: 

                2490     747     675     147
 
PshAI GACnn'nnGTC

Cuts at:      0    1900    3706    4059 
   Size:       1900    1806     353

  Fragments arranged by size: 

                1900    1806     353
 
PstI C_TGCA'G

Cuts at:      0     326     359     461    4013    4059 
   Size:        326      33     102    3552      46

  Fragments arranged by size: 

                3552     326     102      46      33
 
PvuII CAG'CTG

Cuts at:      0     360     456     888    1369    1941    2856    4059 
   Size:        360      96     432     481     572     915    1203

  Fragments arranged by size: 

                1203     915     572     481     432     360      96
 
RsrII CG'GwC_CG

Cuts at:      0    3822    4059 
   Size:       3822     237
 
SacI G_AGCT'C

Cuts at:      0     706    1980    4059 
   Size:        706    1274    2079

  Fragments arranged by size: 

                2079    1274     706
 
SacII CC_GC'GG

Cuts at:      0     754    4059 
   Size:        754    3305
 
SalI G'TCGA_C

Cuts at:      0    2411    4059 
   Size:       2411    1648
 
SapI GCTCTTCn'nnn_

Cuts at:      0     559    4059 
   Size:        559    3500
 
SexAI A'CCwGG_T

Cuts at:      0    3811    4059 
   Size:       3811     248
 
SfcI C'TryA_G

Cuts at:      0     322     355     457    1291    2250    3888    4009    4059 
   Size:        322      33     102     834     959    1638     121      50

  Fragments arranged by size: 

                1638     959     834     322     121     102      50      33
            
 
SfoI GGC'GCC

Cuts at:      0     156     162    2223    4059 
   Size:        156       6    2061    1836

  Fragments arranged by size: 

                2061    1836     156       6
 
SgrAI Cr'CCGG_yG

Cuts at:      0     157    4059 
   Size:        157    3902
 
SmaI CCC'GGG

Cuts at:      0     622    3675    4059 
   Size:        622    3053     384

  Fragments arranged by size: 

                3053     622     384
 
SpeI A'CTAG_T

Cuts at:      0    2661    4059 
   Size:       2661    1398
 
SphI G_CATG'C

Cuts at:      0    1071    2764    4059 
   Size:       1071    1693    1295

  Fragments arranged by size: 

                1693    1295    1071
 
StuI AGG'CCT

Cuts at:      0    1226    2292    4059 
   Size:       1226    1066    1767

  Fragments arranged by size: 

                1767    1226    1066
 
StyI C'CwwG_G

Cuts at:      0    1221    1919    2280    4059 
   Size:       1221     698     361    1779

  Fragments arranged by size: 

                1779    1221     698     361
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     166    1348    4059 
   Size:        166    1182    2711

  Fragments arranged by size: 

                2711    1182     166
 
TatI w'GTAC_w

Cuts at:      0    1013    1652    2130    3755    4059 
   Size:       1013     639     478    1625     304

  Fragments arranged by size: 

                1625    1013     639     478     304
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190    1111    1143    1787    1819    4059 
   Size:        158      32     921      32     644      32    2240

  Fragments arranged by size: 

                2240     921     644     158      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     282     838    1137    4059 
   Size:        282     556     299    2922

  Fragments arranged by size: 

                2922     556     299     282
 
XmaI C'CCGG_G

Cuts at:      0     620    3673    4059 
   Size:        620    3053     386

  Fragments arranged by size: 

                3053     620     386
 
XmnI GAAnn'nnTTC

Cuts at:      0    1384    2675    4059 
   Size:       1384    1291    1384

  Fragments arranged by size: 

                1384    1384    1291
 
ZraI GAC'GTC

Cuts at:      0     940    4059 
   Size:        940    3119
 

 Enzymes that do cut:

     AarI    AatII     AccI     AclI     AcuI   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
    CspCI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FseI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     MfeI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI     PfoI
     PpiI    PpuMI    PshAI     PstI    PvuII    RsrII     SacI    SacII
     SalI     SapI    SexAI     SfcI     SfoI    SgrAI     SmaI     SpeI
     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI
     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AfeI    AflII     AleI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BfrBI     BmtI     BplI    BpuEI
     BsaI    BsaBI    BsiWI    BssSI   BstAPI   BstEII  BstZ17I     ClaI
     DraI   DraIII    EcoRI    EcoRV     FalI    FspAI     KpnI     MluI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PmeI     PmlI
     PsiI   PspOMI    PspXI     PsrI     PvuI    SanDI     SbfI     ScaI
     SfiI     SgfI     SmlI    SnaBI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI
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