Restriction map for pF1KE0163
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflII       
AgeI        AhdI        AlwI        AlwNI       ApaI        
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspMI       
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DpnII       DraI        
DraIII      DrdI        Eam1105I    EarI        EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        Ksp632I     MaeI        
MaeII       MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MscI        MseI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NotI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   77 enzymes) #
###################################

Acc65I        107  GGTACC               G^GTACC
AccIII        218  TCCGGA               T^CCGGA
AcsI          244  GAATTT               [GA]^AATT[CT]
AfaI          108  GTAC                 GT^AC
AflIII        225  ACATGT               A^C[GA][CT]GT
Alw44I        128  GTGCAC               G^TGCAC
AocI            8  CCTTAGG              CC^T[ACGT]AGG
Aor51HI       263  AGCGCT               AGC^GCT
ApaLI         128  GTGCAC               G^TGCAC
ApoI          244  GAATTT               [GA]^AATT[CT]
Asp700I       117  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       107  GGTACC               G^GTACC
AspHI         128  GTGCAC               G[AT]GC[AT]^C
AvaII          14  GGTCC                G^G[AT]CC
BanI          107  GGTACC               G^G[CT][GA]CC
BanII         161  GAGCCC               G[GA]GC[CT]^C
BbvI          187  GCTGC                GCAGC(8/12)
BcgI           24  GCACACTTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI           18  CCCGG                CC^[GC]GG
BpmI          317  CTGGAG               CTGGAG(16/14)
BsaI          295  GGTCTC               GGTCTC(1/5)
BsaWI         218  TCCGGA               [AT]^CCGG[AT]
BseAI         218  TCCGGA               T^CCGGA
BseRI         274  GAGGAG               GAGGAG(10/8)
BsgI          156  GTGCAG               GTGCAG(16/14)
BsiEI          75  CGGCCG               CG[GA][CT]^CG
BsiHKAI       128  GTGCAC               G[AT]GC[AT]^C
BsmAI         296  GTCTC                GTCTC(1/5)
BsmFI          15  GTCCC                GGGAC(10/14)
BspEI         218  TCCGGA               T^CCGGA
BspLU11I      225  ACATGT               A^CATGT
BsrFI          73  ACCGGC               [GA]^CCGG[CT]
BsrI          143  CCAGT                ACTGG(1/-1)
BstEII        138  GGTCACC              G^GT[ACGT]ACC
BstF5I        216  CATCC                GGATG(2/0)
BstNI         316  CCTGG                CC^[AT]GG
BstPI         138  GGTCACC              G^GT[ACGT]ACC
BstXI         309  CCATCAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I          8  CCTTAGG              CC^T[ACGT]AGG
Cac8I         283  GCTGGC               GC[ACGT]^[ACGT]GC
Cfr10I         73  ACCGGC               [GA]^CCGG[CT]
DraII          13  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DsaI           95  CCGCGG               C^C[GA][CT]GG
EagI           75  CGGCCG               C^GGCCG
EclXI          75  CGGCCG               C^GGCCG
Eco47III      263  AGCGCT               AGC^GCT
Eco52I         75  CGGCCG               C^GGCCG
Eco81I          8  CCTTAGG              CC^T[ACGT]AGG
EcoO109I       13  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       138  GGTCACC              G^GT[ACGT]ACC
EcoRII        316  CCTGG                ^CC[AT]GG
FauI           45  GCGGG                CCCGC(4/6)
FokI          216  CATCC                GGATG(9/13)
HaeII         263  AGCGCT               [GA]GCGC^[CT]
HinfI         277  GAGTC                G^A[ACGT]TC
KpnI          107  GGTACC               GGTAC^C
KspI           95  CCGCGG               CCGC^GG
MaeIII        139  GTCAC                ^GT[ACGT]AC
MroI          218  TCCGGA               T^CCGGA
MspA1I         95  CCGCGG               C[AC]G^C[GT]G
MvaI          316  CCTGG                CC^[AT]GG
NciI           18  CCCGG                CC^[GC]GG
NruI           87  TCGCGA               TCG^CGA
NspI          225  ACATGT               [GA]CATG^[CT]
PleI          277  GAGTC                GAGTC(4/5)
PpuMI          13  GGGTCCC              [GA]G^G[AT]CC[CT]
RsaI          108  GTAC                 GT^AC
SacII          95  CCGCGG               CCGC^GG
SfaNI         215  GCATC                GCATC(5/9)
SmlI          298  CTCAAG               C^T[CT][GA]AG
TaqI           33  TCGA                 T^CGA
TseI          187  GCTGC                G^C[AT]GC
Tsp45I        139  GTCAC                ^GT[GC]AC
Tsp509I       245  AATT                 ^AATT
TspRI         144  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        33  TCGA                 T^CGA
XmnI          117  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AluI          173  AGCT                 AG^CT
              186  AGCT                
BmyI          128  GTGCAC               G[AGT]GC[ACT]^C
              161  GAGCCC              
BsaJI          17  CCCCGG               C^C[ACGT][ACGT]GG
               95  CCGCGG              
Bsp1286I      128  GTGCAC               G[AGT]GC[ACT]^C
              161  GAGCCC              
BssKI          18  CCCGG                ^CC[ACGT]GG
              316  CCTGG               
Cfr13I         14  GGTCC                G^G[ACGT]CC
              231  GGCCC               
DdeI            9  CTTAG                C^T[ACGT]AG
               61  CTGAG               
EaeI           68  CGGCCA               [CT]^GGCC[GA]
               75  CGGCCG              
EcoNI         111  CCTTGTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              234  CCTACATCAGG         
HphI          259  GGTGA                GGTGA(8/7)
              140  TCACC               
Sau96I         14  GGTCC                G^G[ACGT]CC
              231  GGCCC               
ScrFI          18  CCCGG                CC^[ACGT]GG
              316  CCTGG               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AccII       AciI        BsiYI       BslI        BstUI       
CfoI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HpaII       ItaI        MnlI        MspI        
MvnI        MwoI        NlaIII      NlaIV       
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