Restriction map for pF1KE0521
##################################
# NO CUT ENZYMES (  140 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BanII       BbeI        BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BpuAI       BsaBI       
BsaWI       BseAI       BseRI       BsiEI       BsiWI       
BsmFI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluNI       MroI        MscI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII         394  GACGTC               GACGT^C
AcsI           82  GAATTC               [GA]^AATT[CT]
AhdI          301  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         544  CAGGCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI           82  GAATTC               [GA]^AATT[CT]
AspEI         301  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          254  CCCGAG               C^[CT]CG[GA]G
BcnI          618  CCCGG                CC^[GC]GG
BsgI          649  GTGCAG               GTGCAG(16/14)
BsmBI         392  GAGACG               CGTCTC(1/5)
BsoBI         254  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      344  TGTACA               T^GTACA
BspMI         227  ACCTGC               ACCTGC(4/8)
BsrGI         344  TGTACA               T^GTACA
BstAPI        651  GCAGCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         134  AGATCC               [GA]^GATC[CT]
DraII         779  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      301  GACAGCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         315  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      779  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI          82  GAATTC               G^AATTC
EcoT14I       554  CCAAGG               C^C[AT][AT]GG
HaeII         546  GGCGCT               [GA]GCGC^[CT]
HapII         619  CCGG                 C^CGG
HgaI          444  GACGC                GACGC(5/10)
HinfI         483  GACTC                G^A[ACGT]TC
HpaII         619  CCGG                 C^CGG
MflI          134  AGATCC               [GA]^GATC[CT]
MluI           47  ACGCGT               A^CGCGT
MseI           17  TTAA                 T^TAA
MslI          291  CACTGACGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          619  CCGG                 C^CGG
NciI          618  CCCGG                CC^[GC]GG
PleI          483  GACTC                GAGTC(4/5)
PpuMI         779  AGGACCT              [GA]G^G[AT]CC[CT]
SapI          516  GCTCTTC              GCTCTTC(1/4)
SspBI         344  TGTACA               T^GTACA
StyI          554  CCAAGG               C^C[AT][AT]GG
TaqI          626  TCGA                 T^CGA
Tru9I          17  TTAA                 T^TAA
Tsp509I        83  AATT                 ^AATT
TthHB8I       626  TCGA                 T^CGA
XcmI          339  CCATCTGTACAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         134  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI          394  GACGTC               G[GA]^CG[CT]C
              444  GACGCC              
AflIII         47  ACGCGT               A^C[GA][CT]GT
              196  ACACGT              
AvaII         630  GGACC                G^G[AT]CC
              780  GGACC               
BanI          451  GGCACC               G^G[CT][GA]CC
              703  GGCACC              
BbrPI         197  CACGTG               CAC^GTG
              745  CACGTG              
BlpI          462  GCTGAGC              GC^T[ACGT]AGC
              660  GCTGAGC             
Bpu1102I      462  GCTGAGC              GC^T[ACGT]AGC
              660  GCTGAGC             
BsaHI         394  GACGTC               G[GA]^CG[CT]C
              444  GACGCC              
BsaI          796  GAGACC               GGTCTC(1/5)
              324  GGTCTC              
BsrI          245  ACTGG                ACTGG(1/-1)
              791  ACTGG               
BstXI         290  CCACTGACGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              572  CCAGCCTCCTGG        
CelII         462  GCTGAGC              GC^T[ACGT]AGC
              660  GCTGAGC             
EaeI          620  CGGCCA               [CT]^GGCC[GA]
              741  CGGCCA              
EarI          517  CTCTTC               CTCTTC(1/4)
              598  GAAGAG              
Hin1I         394  GACGTC               G[GA]^CG[CT]C
              444  GACGCC              
Ksp632I       517  CTCTTC               CTCTTC(1/4)
              598  GAAGAG              
MaeIII        274  GTCAC                ^GT[ACGT]AC
              748  GTGAC               
MspA1I        238  CAGCTG               C[AC]G^C[GT]G
              607  CAGCTG              
PmaCI         197  CACGTG               CAC^GTG
              745  CACGTG              
PmlI          197  CACGTG               CAC^GTG
              745  CACGTG              
PstI          229  CTGCAG               CTGCA^G
              820  CTGCAG              
PvuII         238  CAGCTG               CAG^CTG
              607  CAGCTG              
Tsp45I        274  GTCAC                ^GT[GC]AC
              748  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AspHI       BbvI        BmyI        BpmI        BsaAI       
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HphI        ItaI        MaeII       MboI        
MboII       MnlI        MvaI        MvnI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        TseI        
TspRI       
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