Restriction map for pF1KE0521
 (Linear) (Six-base) MAPSORT of: KIEE0521.seq  Check: 472  from: 1  to: 828



KIEE0521.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AatII G_ACGT'C

Cuts at:      0     398     828 
   Size:        398     430
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     828 
   Size:        337     491
 
AflIII A'CryG_T

Cuts at:      0      47     196     828 
   Size:         47     149     632

  Fragments arranged by size: 

                 632     149      47
 
AhdI GACnn_n'nnGTC

Cuts at:      0     306     828 
   Size:        306     522
 
AleI CACnn'nnGTG

Cuts at:      0     295     828 
   Size:        295     533
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     490     828 
   Size:        490     338
 
AlwNI CAG_nnn'CTG

Cuts at:      0     549     828 
   Size:        549     279
 
ApoI r'AATT_y

Cuts at:      0      82     828 
   Size:         82     746
 
AvaI C'yCGr_G

Cuts at:      0     254     828 
   Size:        254     574
 
BanI G'GyrC_C

Cuts at:      0     451     703     828 
   Size:        451     252     125

  Fragments arranged by size: 

                 451     252     125
 
BlpI GC'TnA_GC

Cuts at:      0     463     661     828 
   Size:        463     198     167

  Fragments arranged by size: 

                 463     198     167
 
BmgBI CAC'GTC

Cuts at:      0     297     828 
   Size:        297     531
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     801     828 
   Size:        801      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     510     544     828 
   Size:        499      11      34     284

  Fragments arranged by size: 

                 499     284      34      11
 
Bpu10I CC'TnA_GC

Cuts at:      0     376     828 
   Size:        376     452
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     330     790     828 
   Size:        330     460      38

  Fragments arranged by size: 

                 460     330      38
 
BsaAI yAC'GTr

Cuts at:      0     199     648     747     828 
   Size:        199     449      99      81

  Fragments arranged by size: 

                 449     199      99      81
 
BsaHI Gr'CG_yC

Cuts at:      0     395     445     828 
   Size:        395      50     383

  Fragments arranged by size: 

                 395     383      50
 
BseYI C'CCAG_C

Cuts at:      0     183     828 
   Size:        183     645
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     828 
   Size:        670     158
 
BsiHKAI G_wGCw'C

Cuts at:      0     382     566     590     828 
   Size:        382     184      24     238

  Fragments arranged by size: 

                 382     238     184      24
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     386     828 
   Size:        386     442
 
Bsp1286I G_dGCh'C

Cuts at:      0     382     566     590     828 
   Size:        382     184      24     238

  Fragments arranged by size: 

                 382     238     184      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     236     828 
   Size:        236     592
 
BsrGI T'GTAC_A

Cuts at:      0     344     828 
   Size:        344     484
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     657     828 
   Size:        657     171
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     297     579     828 
   Size:        297     282     249

  Fragments arranged by size: 

                 297     282     249
 
BstYI r'GATC_y

Cuts at:      0     134     828 
   Size:        134     694
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     552     828 
   Size:        552     276
 
BtsI GCAGTG_nn'

Cuts at:      0     817     828 
   Size:        817      11
 
EaeI y'GGCC_r

Cuts at:      0     620     741     828 
   Size:        620     121      87

  Fragments arranged by size: 

                 620     121      87
 
EarI CTCTTCn'nnn_

Cuts at:      0     523     593     828 
   Size:        523      70     235

  Fragments arranged by size: 

                 523     235      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     499     510     544     828 
   Size:        337     162      11      34     284

  Fragments arranged by size: 

                 337     284     162      34      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     319     828 
   Size:        319     509
 
EcoO109I rG'GnC_Cy

Cuts at:      0     780     828 
   Size:        780      48
 
EcoRI G'AATT_C

Cuts at:      0      82     828 
   Size:         82     746
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     519     534     828 
   Size:        519      15     294

  Fragments arranged by size: 

                 519     294      15
 
HaeII r_GCGC'y

Cuts at:      0     550     828 
   Size:        550     278
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     292     324     828 
   Size:        292      32     504

  Fragments arranged by size: 

                 504     292      32
 
MluI A'CGCG_T

Cuts at:      0      47     828 
   Size:         47     781
 
MslI CAynn'nnrTG

Cuts at:      0     295     828 
   Size:        295     533
 
MspA1I CmG'CkG

Cuts at:      0     240     609     828 
   Size:        240     369     219

  Fragments arranged by size: 

                 369     240     219
 
PfoI T'CCnGG_A

Cuts at:      0     521     578     828 
   Size:        521      57     250

  Fragments arranged by size: 

                 521     250      57
 
PmlI CAC'GTG

Cuts at:      0     199     747     828 
   Size:        199     548      81

  Fragments arranged by size: 

                 548     199      81
 
PpuMI rG'GwC_Cy

Cuts at:      0     780     828 
   Size:        780      48
 
PstI C_TGCA'G

Cuts at:      0     233     824     828 
   Size:        233     591       4

  Fragments arranged by size: 

                 591     233       4
 
PvuII CAG'CTG

Cuts at:      0     240     609     828 
   Size:        240     369     219

  Fragments arranged by size: 

                 369     240     219
 
SapI GCTCTTCn'nnn_

Cuts at:      0     523     828 
   Size:        523     305
 
SfcI C'TryA_G

Cuts at:      0      87     229     820     828 
   Size:         87     142     591       8

  Fragments arranged by size: 

                 591     142      87       8
 
StyI C'CwwG_G

Cuts at:      0     554     828 
   Size:        554     274
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     292     828 
   Size:        292     536
 
TatI w'GTAC_w

Cuts at:      0     344     828 
   Size:        344     484
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     346     828 
   Size:        346     482
 
ZraI GAC'GTC

Cuts at:      0     396     828 
   Size:        396     432
 

 Enzymes that do cut:

    AatII     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApoI
     AvaI     BanI     BlpI    BmgBI     BmrI     BpmI   Bpu10I     BsaI
    BsaAI    BsaHI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI
    BsrGI   BstAPI    BstXI    BstYI    BtgZI     BtsI     EaeI     EarI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I     MluI
     MslI   MspA1I     PfoI     PmlI    PpuMI     PstI    PvuII     SapI
     SfcI     StyI    TaqII     TatI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII Bme1580I     BmtI     BplI    BpuEI    BsaBI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com