Restriction map for pF1KE0476
##################################
# NO CUT ENZYMES (  142 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       AvrII       BbeI        
BbrPI       BcgI        BciVI       BfaI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HhaI        
HinP1I      HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MroI        
MseI        MslI        MunI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TaqI        
Tru9I       TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccI          505  GTCTAC               GT^[AC][GT]AC
AcsI          349  AAATTC               [GA]^AATT[CT]
AcyI          556  GGCGTC               G[GA]^CG[CT]C
AocI           69  CCTCAGG              CC^T[ACGT]AGG
ApoI          349  AAATTC               [GA]^AATT[CT]
AspHI         459  GAGCAC               G[AT]GC[AT]^C
AspI          700  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          579  TGGCCA               TGG^CCA
BbsI          706  GTCTTC               GAAGAC(2/6)
BbvCI         624  GCTGAGG              CCTCAGC(-5/-2)
BclI          410  TGATCA               T^GATCA
BglI          299  GCCCCATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          314  CTGGAG               CTGGAG(16/14)
BpuAI         706  GTCTTC               GAAGAC(2/6)
BsaHI         556  GGCGTC               G[GA]^CG[CT]C
BsgI          395  CTGCAC               GTGCAG(16/14)
BsiHKAI       459  GAGCAC               G[AT]GC[AT]^C
BssSI         456  CACGAG               CACGAG(-5/-1)
BstEII        567  GGTCACC              G^GT[ACGT]ACC
BstPI         567  GGTCACC              G^GT[ACGT]ACC
Bsu36I         69  CCTCAGG              CC^T[ACGT]AGG
EarI          724  CTCTTC               CTCTTC(1/4)
Eco81I         69  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       567  GGTCACC              G^GT[ACGT]ACC
FauI          438  CCCGC                CCCGC(4/6)
FbaI          410  TGATCA               T^GATCA
Hin1I         556  GGCGTC               G[GA]^CG[CT]C
HincII        604  GTCAAC               GT[CT]^[GA]AC
HindII        604  GTCAAC               GT[CT]^[GA]AC
Ksp632I       724  CTCTTC               CTCTTC(1/4)
MluI          512  ACGCGT               A^CGCGT
MluNI         579  TGGCCA               TGG^CCA
MscI          579  TGGCCA               TGG^CCA
MspA1I        552  CAGCGG               C[AC]G^C[GT]G
PflFI         700  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI          723  GCTCTTC              GCTCTTC(1/4)
SmaI          196  CCCGGG               CCC^GGG
Tsp509I       350  AATT                 ^AATT
Tth111I       700  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         196  CCCGGG               C^CCGGG
XmaI          196  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccII         423  CGCG                 CG^CG
              513  CGCG                
AfaI          147  GTAC                 GT^AC
              546  GTAC                
AflIII        512  ACGCGT               A^C[GA][CT]GT
              584  ACACGT              
AluI           16  AGCT                 AG^CT
               41  AGCT                
ApaI           10  GGGCCC               GGGCC^C
              163  GGGCCC              
AvaI          196  CCCGGG               C^[CT]CG[GA]G
              609  CTCGGG              
BamHI         101  GGATCC               G^GATCC
              200  GGATCC              
BanI          129  GGCACC               G^G[CT][GA]CC
              291  GGTGCC              
BseRI         328  GAGGAG               GAGGAG(10/8)
              643  GAGGAG              
BsmFI         270  GGGAC                GGGAC(10/14)
              484  GTCCC               
BsoBI         196  CCCGGG               C^[CT]CG[GA]G
              609  CTCGGG              
BspMI         475  ACCTGC               ACCTGC(4/8)
              633  GCAGGT              
BsrI          463  ACTGG                ACTGG(1/-1)
              354  CCAGT               
BstUI         423  CGCG                 CG^CG
              513  CGCG                
BstYI         101  GGATCC               [GA]^GATC[CT]
              200  GGATCC              
DdeI           70  CTCAG                C^T[ACGT]AG
              625  CTGAG               
DsaI          266  CCATGG               C^C[GA][CT]GG
              302  CCATGG              
EaeI          243  CGGCCA               [CT]^GGCC[GA]
              579  TGGCCA              
HinfI         182  GAATC                G^A[ACGT]TC
              416  GATTC               
MaeII           5  ACGT                 A^CGT
              586  ACGT                
MaeIII        370  GTCAC                ^GT[ACGT]AC
              568  GTCAC               
MboII         707  TCTTC                GAAGA(8/7)
              725  TCTTC               
MflI          101  GGATCC               [GA]^GATC[CT]
              200  GGATCC              
MvnI          423  CGCG                 CG^CG
              513  CGCG                
NcoI          266  CCATGG               C^CATGG
              302  CCATGG              
PpuMI         207  AGGACCC              [GA]G^G[AT]CC[CT]
              270  GGGACCC             
RsaI          147  GTAC                 GT^AC
              546  GTAC                
TfiI          182  GAATC                G^A[AT]TC
              416  GATTC               
Tsp45I        370  GTCAC                ^GT[GC]AC
              568  GTCAC               
TspRI         359  CAGTG                CA[GC]TG[ACGT][ACGT]^
              462  CACTG               
XhoII         101  GGATCC               [GA]^GATC[CT]
              200  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AlwI        AvaII       BanII       BbvI        
BcnI        BmyI        BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DpnII       DraII       EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HgaI        HpaII       HphI        ItaI        MboI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com