Restriction map for pF1KE0476
 (Linear) (Six-base) MAPSORT of: KIEE0476.seq  Check: 7732  from: 1  to: 735



KIEE0476.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     484     624     735 
   Size:        484     140     111

  Fragments arranged by size: 

                 484     140     111
 
AccI GT'mk_AC

Cuts at:      0     506     735 
   Size:        506     229
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     735 
   Size:         58     677
 
AflIII A'CryG_T

Cuts at:      0     512     584     735 
   Size:        512      72     151

  Fragments arranged by size: 

                 512     151      72
 
ApaI G_GGCC'C

Cuts at:      0      14     167     735 
   Size:         14     153     568

  Fragments arranged by size: 

                 568     153      14
 
ApoI r'AATT_y

Cuts at:      0     349     735 
   Size:        349     386
 
AvaI C'yCGr_G

Cuts at:      0     196     609     735 
   Size:        196     413     126

  Fragments arranged by size: 

                 413     196     126
 
BamHI G'GATC_C

Cuts at:      0     101     200     735 
   Size:        101      99     535

  Fragments arranged by size: 

                 535     101      99
 
BanI G'GyrC_C

Cuts at:      0     129     291     735 
   Size:        129     162     444

  Fragments arranged by size: 

                 444     162     129
 
BanII G_rGCy'C

Cuts at:      0      14     167     697     735 
   Size:         14     153     530      38

  Fragments arranged by size: 

                 530     153      38      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     699     735 
   Size:        699      36
 
BbvCI CC'TCA_GC

Cuts at:      0     625     735 
   Size:        625     110
 
BclI T'GATC_A

Cuts at:      0     410     735 
   Size:        410     325
 
BglI GCCn_nnn'nGGC

Cuts at:      0     305     735 
   Size:        305     430
 
Bme1580I G_kGCm'C

Cuts at:      0      14     132     167     296     735 
   Size:         14     118      35     129     439

  Fragments arranged by size: 

                 439     129     118      35      14
 
BmgBI CAC'GTC

Cuts at:      0       6     587     735 
   Size:          6     581     148

  Fragments arranged by size: 

                 581     148       6
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     335     735 
   Size:        335     400
 
Bpu10I CC'TnA_GC

Cuts at:      0     625     735 
   Size:        625     110
 
BsaHI Gr'CG_yC

Cuts at:      0     557     735 
   Size:        557     178
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     343     658     735 
   Size:        343     315      77

  Fragments arranged by size: 

                 343     315      77
 
BseYI C'CCAG_C

Cuts at:      0      13     186     735 
   Size:         13     173     549

  Fragments arranged by size: 

                 549     173      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     735 
   Size:        380     355
 
BsiHKAI G_wGCw'C

Cuts at:      0     463     735 
   Size:        463     272
 
Bsp1286I G_dGCh'C

Cuts at:      0      14     132     167     296     463     697     735 
   Size:         14     118      35     129     167     234      38

  Fragments arranged by size: 

                 234     167     129     118      38      35      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     484     624     735 
   Size:        484     140     111

  Fragments arranged by size: 

                 484     140     111
 
BssSI C'ACGA_G

Cuts at:      0     456     735 
   Size:        456     279
 
BstEII G'GTnAC_C

Cuts at:      0     567     735 
   Size:        567     168
 
BstYI r'GATC_y

Cuts at:      0     101     200     735 
   Size:        101      99     535

  Fragments arranged by size: 

                 535     101      99
 
Bsu36I CC'TnA_GG

Cuts at:      0      70     735 
   Size:         70     665
 
BtgI C'CryG_G

Cuts at:      0     266     302     735 
   Size:        266      36     433

  Fragments arranged by size: 

                 433     266      36
 
EaeI y'GGCC_r

Cuts at:      0     243     579     735 
   Size:        243     336     156

  Fragments arranged by size: 

                 336     243     156
 
EarI CTCTTCn'nnn_

Cuts at:      0     730     735 
   Size:        730       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     335     735 
   Size:         58     277     400

  Fragments arranged by size: 

                 400     277      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10     163     208     271     735 
   Size:         10     153      45      63     464

  Fragments arranged by size: 

                 464     153      63      45      10
 
HincII GTy'rAC

Cuts at:      0     606     735 
   Size:        606     129
 
MluI A'CGCG_T

Cuts at:      0     512     735 
   Size:        512     223
 
MscI TGG'CCA

Cuts at:      0     581     735 
   Size:        581     154
 
MspA1I CmG'CkG

Cuts at:      0     554     735 
   Size:        554     181
 
NcoI C'CATG_G

Cuts at:      0     266     302     735 
   Size:        266      36     433

  Fragments arranged by size: 

                 433     266      36
 
PasI CC'CwG_GG

Cuts at:      0     106     277     735 
   Size:        106     171     458

  Fragments arranged by size: 

                 458     171     106
 
PpuMI rG'GwC_Cy

Cuts at:      0     208     271     735 
   Size:        208      63     464

  Fragments arranged by size: 

                 464     208      63
 
PspOMI G'GGCC_C

Cuts at:      0      10     163     735 
   Size:         10     153     572

  Fragments arranged by size: 

                 572     153      10
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     221     253     735 
   Size:        221      32     482

  Fragments arranged by size: 

                 482     221      32
 
SanDI GG'GwC_CC

Cuts at:      0     271     735 
   Size:        271     464
 
SapI GCTCTTCn'nnn_

Cuts at:      0     730     735 
   Size:        730       5
 
SmaI CCC'GGG

Cuts at:      0     198     735 
   Size:        198     537
 
StyI C'CwwG_G

Cuts at:      0      30     167     266     302     735 
   Size:         30     137      99      36     433

  Fragments arranged by size: 

                 433     137      99      36      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     414     735 
   Size:        414     321
 
Tth111I GACn'n_nGTC

Cuts at:      0     703     735 
   Size:        703      32
 
XmaI C'CCGG_G

Cuts at:      0     196     735 
   Size:        196     539
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI
     BpmI   Bpu10I    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BssSI   BstEII    BstYI   Bsu36I     BtgI     EaeI     EarI
  Eco57MI EcoO109I   HincII     MluI     MscI   MspA1I     NcoI     PasI
    PpuMI   PspOMI     PsrI    SanDI     SapI     SmaI     StyI    TaqII
  Tth111I     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstXI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MmeI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI     TstI     XbaI     XcmI     XhoI
     XmnI     ZraI
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