Restriction map for pF1KE0355
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AflIII      
AgeI        Aor51HI     ApoI        AscI        AseI        
AsnI        Asp700I     AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BclI        BfrI        
BlnI        BsaBI       BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BssHII      BstAPI      BstEII      BstPI       
ClaI        CpoI        DraI        DraIII      DrdI        
EarI        Eco47III    EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HpaI        KasI        Ksp632I     MamI        MfeI        
MluI        MroI        MseI        MunI        NarI        
NcoI        NdeI        NotI        NruI        NsiI        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    RcaI        RsrII       SapI        SbfI        
ScaI        SexAI       SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    SspI        SwaI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        1164  GACGTC               GACGT^C
Acc65I       1802  GGTACC               G^GTACC
AhdI         1550  GACTGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1393  GTGCAC               G^TGCAC
ApaLI        1393  GTGCAC               G^TGCAC
Asp718I      1802  GGTACC               G^GTACC
AspEI        1550  GACTGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1886  TGGCCA               TGG^CCA
BcgI          668  GCAGCCAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         897  GTATCC               GTATCC(6/5)
BglII         386  AGATCT               A^GATCT
BlpI          828  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      828  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1858  TACGTG               [CT]AC^GT[GA]
BsmI         1443  GAATGC               GAATGC(1/-1)
BsrDI         994  CATTGC               GCAATG(2/0)
Bst1107I      340  GTATAC               GTA^TAC
BstBI        1288  TTCGAA               TT^CGAA
BstXI          68  CCATCGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       340  GTATAC               GTA^TAC
CelII         828  GCTCAGC              GC^T[ACGT]AGC
Eam1105I     1550  GACTGCTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI          438  GACGC                GACGC(5/10)
HincII        249  GTCGAC               GT[CT]^[GA]AC
HindII        249  GTCGAC               GT[CT]^[GA]AC
KpnI         1802  GGTACC               GGTAC^C
MluNI        1886  TGGCCA               TGG^CCA
MscI         1886  TGGCCA               TGG^CCA
NheI          219  GCTAGC               G^CTAGC
NspI          998  GCATGC               [GA]CATG^[CT]
NspV         1288  TTCGAA               TT^CGAA
PleI          412  GAGTC                GAGTC(4/5)
PvuI          848  CGATCG               CGAT^CG
SalI          249  GTCGAC               G^TCGAC
SfuI         1288  TTCGAA               TT^CGAA
SmlI          775  CTCAAG               C^T[CT][GA]AG
SphI          998  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          438  GACGCC               G[GA]^CG[CT]C
             1164  GACGTC              
AlwI         1242  GATCC                GGATC(4/5)
             1610  GGATC               
BanI         1699  GGTGCC               G^G[CT][GA]CC
             1802  GGTACC              
BbvCI        1753  CCTCAGC              CCTCAGC(-5/-2)
             1737  GCTGAGG             
BsaHI         438  GACGCC               G[GA]^CG[CT]C
             1164  GACGTC              
BsaI          816  GGTCTC               GGTCTC(1/5)
             1082  GGTCTC              
Bsp1407I      884  TGTACA               T^GTACA
             1382  TGTACA              
BspMI         824  ACCTGC               ACCTGC(4/8)
             1709  ACCTGC              
BsrBI        1171  GAGCGG               CCGCTC(-3/-3)
             1743  GAGCGG              
BsrGI         884  TGTACA               T^GTACA
             1382  TGTACA              
BssSI        1795  CACGAG               CACGAG(-5/-1)
              802  CTCGTG              
BstYI         386  AGATCT               [GA]^GATC[CT]
             1241  AGATCC              
EcoT14I      1313  CCAAGG               C^C[AT][AT]GG
             1502  CCAAGG              
Hin1I         438  GACGCC               G[GA]^CG[CT]C
             1164  GACGTC              
HphI           27  GGTGA                GGTGA(8/7)
             1090  TCACC               
KspI         1030  CCGCGG               CCGC^GG
             1229  CCGCGG              
MaeII        1165  ACGT                 A^CGT
             1859  ACGT                
MflI          386  AGATCT               [GA]^GATC[CT]
             1241  AGATCC              
MslI          224  CACAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              331  CACCGCCGTG          
NaeI          322  GCCGGC               GCC^GGC
              629  GCCGGC              
NgoMIV        322  GCCGGC               G^CCGGC
              629  GCCGGC              
PpuMI         791  GGGACCT              [GA]G^G[AT]CC[CT]
             1142  AGGACCT             
SacI         1432  GAGCTC               GAGCT^C
             1543  GAGCTC              
SacII        1030  CCGCGG               CCGC^GG
             1229  CCGCGG              
SfiI            3  GGCCGCTGCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1500  GGCCAAGGGGGCC       
SspBI         884  TGTACA               T^GTACA
             1382  TGTACA              
StyI         1313  CCAAGG               C^C[AT][AT]GG
             1502  CCAAGG              
TfiI          465  GAATC                G^A[AT]TC
              783  GAATC               
XhoII         386  AGATCT               [GA]^GATC[CT]
             1241  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AluI        
AlwNI       AocI        ApaI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       Bsu36I      Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EclXI       Eco52I      Eco81I      
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HindIII     HinfI       HpaII       ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StuI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     XmaCI       XmaI        
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