Restriction map for pF1KE0355
 (Linear) (Six-base) MAPSORT of: KIEE0355.seq  Check: 158  from: 1  to: 1893



KIEE0355.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     833    1893 
   Size:        833    1060
 
AatII G_ACGT'C

Cuts at:      0    1168    1893 
   Size:       1168     725
 
AccI GT'mk_AC

Cuts at:      0     250     341    1256    1893 
   Size:        250      91     915     637

  Fragments arranged by size: 

                 915     637     250      91
 
Acc65I G'GTAC_C

Cuts at:      0    1802    1893 
   Size:       1802      91
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     301     385     496    1243    1893 
   Size:        235      66      84     111     747     650

  Fragments arranged by size: 

                 747     650     235     111      84      66
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1555    1893 
   Size:       1555     338
 
AleI CACnn'nnGTG

Cuts at:      0     228     335    1893 
   Size:        228     107    1558

  Fragments arranged by size: 

                1558     228     107
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1120    1152    1893 
   Size:       1120      32     741

  Fragments arranged by size: 

                1120     741      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     234     474     861    1158    1221    1893 
   Size:        234     240     387     297      63     672

  Fragments arranged by size: 

                 672     387     297     240     234      63
 
ApaI G_GGCC'C

Cuts at:      0     246     711    1673    1893 
   Size:        246     465     962     220

  Fragments arranged by size: 

                 962     465     246     220
 
ApaLI G'TGCA_C

Cuts at:      0    1393    1893 
   Size:       1393     500
 
AvaI C'yCGr_G

Cuts at:      0      95     382     593    1012    1403    1874    1893 
   Size:         95     287     211     419     391     471      19

  Fragments arranged by size: 

                 471     419     391     287     211      95      19
 
BanI G'GyrC_C

Cuts at:      0    1699    1802    1893 
   Size:       1699     103      91

  Fragments arranged by size: 

                1699     103      91
 
BanII G_rGCy'C

Cuts at:      0     246     711    1019    1208    1404    1436    1547    1673 
   Size:        246     465     308     189     196      32     111     126

Cuts at:   1673    1893 
   Size:        220

  Fragments arranged by size: 

                 465     308     246     220     196     189     126     111
                  32
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     538    1231    1637    1893 
   Size:        538     693     406     256

  Fragments arranged by size: 

                 693     538     406     256
 
BbvCI CC'TCA_GC

Cuts at:      0    1738    1754    1893 
   Size:       1738      16     139

  Fragments arranged by size: 

                1738     139      16
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     657     691    1893 
   Size:        657      34    1202

  Fragments arranged by size: 

                1202     657      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     908    1893 
   Size:        908     985
 
BglI GCCn_nnn'nGGC

Cuts at:      0      10      64     499    1408    1507    1893 
   Size:         10      54     435     909      99     386

  Fragments arranged by size: 

                 909     435     386      99      54      10
 
BglII A'GATC_T

Cuts at:      0     386    1893 
   Size:        386    1507
 
BlpI GC'TnA_GC

Cuts at:      0     829    1893 
   Size:        829    1064
 
Bme1580I G_kGCm'C

Cuts at:      0     194     246     711    1397    1673    1851    1893 
   Size:        194      52     465     686     276     178      42

  Fragments arranged by size: 

                 686     465     276     194     178      52      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     240    1893 
   Size:        240    1653
 
BmtI G_CTAG'C

Cuts at:      0     223    1893 
   Size:        223    1670
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     379     471    1893 
   Size:        379      92    1422

  Fragments arranged by size: 

                1422     379      92
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     466     592     625     748    1168    1582    1606 
   Size:        256     210     126      33     123     420     414      24

Cuts at:   1606    1801    1893 
   Size:        195      92

  Fragments arranged by size: 

                 420     414     256     210     195     126     123      92
                  33      24
 
Bpu10I CC'TnA_GC

Cuts at:      0    1417    1738    1754    1852    1893 
   Size:       1417     321      16      98      41

  Fragments arranged by size: 

                1417     321      98      41      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     760    1893 
   Size:        760    1133
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     822    1088    1893 
   Size:        822     266     805

  Fragments arranged by size: 

                 822     805     266
 
BsaAI yAC'GTr

Cuts at:      0    1860    1893 
   Size:       1860      33
 
BsaHI Gr'CG_yC

Cuts at:      0     439    1165    1893 
   Size:        439     726     728

  Fragments arranged by size: 

                 728     726     439
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     121     151    1893 
   Size:        121      30    1742

  Fragments arranged by size: 

                1742     121      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     135     463     613     637    1252    1471    1755    1798 
   Size:        135     328     150      24     615     219     284      43

Cuts at:   1798    1831    1893 
   Size:         33      62

  Fragments arranged by size: 

                 615     328     284     219     150     135      62      43
                  33      24
 
BseYI C'CCAG_C

Cuts at:      0     178     314     514    1018    1208    1893 
   Size:        178     136     200     504     190     685

  Fragments arranged by size: 

                 685     504     200     190     178     136
 
BsiEI CG_ry'CG

Cuts at:      0      14     257     327     851    1893 
   Size:         14     243      70     524    1042

  Fragments arranged by size: 

                1042     524     243      70      14
 
BsiHKAI G_wGCw'C

Cuts at:      0     880    1397    1436    1547    1893 
   Size:        880     517      39     111     346

  Fragments arranged by size: 

                 880     517     346     111      39
 
BsmI GAATG_Cn'

Cuts at:      0    1449    1893 
   Size:       1449     444
 
Bsp1286I G_dGCh'C

Cuts at:      0     194     246     711     880    1019    1208    1397    1404 
   Size:        194      52     465     169     139     189     189       7

Cuts at:   1404    1436    1547    1673    1851    1893 
   Size:         32     111     126     178      42

  Fragments arranged by size: 

                 465     194     189     189     178     169     139     126
                 111      52      42      32       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     833    1718    1893 
   Size:        833     885     175

  Fragments arranged by size: 

                 885     833     175
 
BsrBI CCG'CTC

Cuts at:      0    1173    1745    1893 
   Size:       1173     572     148

  Fragments arranged by size: 

                1173     572     148
 
BsrDI GCAATG_nn'

Cuts at:      0     993    1893 
   Size:        993     900
 
BsrFI r'CCGG_y

Cuts at:      0      82      89     322     629    1893 
   Size:         82       7     233     307    1264

  Fragments arranged by size: 

                1264     307     233      82       7
 
BsrGI T'GTAC_A

Cuts at:      0     884    1382    1893 
   Size:        884     498     511

  Fragments arranged by size: 

                 884     511     498
 
BssSI C'ACGA_G

Cuts at:      0     802    1795    1893 
   Size:        802     993      98

  Fragments arranged by size: 

                 993     802      98
 
BstBI TT'CG_AA

Cuts at:      0    1289    1893 
   Size:       1289     604
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      75    1893 
   Size:         75    1818
 
BstYI r'GATC_y

Cuts at:      0     386    1241    1893 
   Size:        386     855     652

  Fragments arranged by size: 

                 855     652     386
 
BstZ17I GTA'TAC

Cuts at:      0     342    1893 
   Size:        342    1551
 
Bsu36I CC'TnA_GG

Cuts at:      0     118     862    1496    1893 
   Size:        118     744     634     397

  Fragments arranged by size: 

                 744     634     397     118
 
BtgI C'CryG_G

Cuts at:      0    1030    1076    1188    1229    1893 
   Size:       1030      46     112      41     664

  Fragments arranged by size: 

                1030     664     112      46      41
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1665    1893 
   Size:       1665     228
 
BtsI GCAGTG_nn'

Cuts at:      0     235     880    1837    1893 
   Size:        235     645     957      56

  Fragments arranged by size: 

                 957     645     235      56
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1443    1478    1893 
   Size:       1443      35     415

  Fragments arranged by size: 

                1443     415      35
 
EaeI y'GGCC_r

Cuts at:      0       2      11     254     324     751    1886    1893 
   Size:          2       9     243      70     427    1135       7

  Fragments arranged by size: 

                1135     427     243      70       9       7       2
 
EagI C'GGCC_G

Cuts at:      0      11     254     324    1893 
   Size:         11     243      70    1569

  Fragments arranged by size: 

                1569     243      70      11
 
EcoICRI GAG'CTC

Cuts at:      0    1434    1545    1893 
   Size:       1434     111     348

  Fragments arranged by size: 

                1434     348     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     256     301     385     466     496     592     625 
   Size:        235      21      45      84      81      30      96      33

Cuts at:    625     748    1168    1243    1582    1606    1801    1893 
   Size:        123     420      75     339      24     195      92

  Fragments arranged by size: 

                 420     339     235     195     123      96      92      84
                  81      75      45      33      30      24      21
 
EcoO109I rG'GnC_Cy

Cuts at:      0     242     708     792    1143    1670    1893 
   Size:        242     466      84     351     527     223

  Fragments arranged by size: 

                 527     466     351     242     223      84
 
HaeII r_GCGC'y

Cuts at:      0      47     630     893    1893 
   Size:         47     583     263    1000

  Fragments arranged by size: 

                1000     583     263      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     534     566    1093    1125    1541    1573    1893 
   Size:        534      32     527      32     416      32     320

  Fragments arranged by size: 

                 534     527     416     320      32      32      32
 
HincII GTy'rAC

Cuts at:      0     251    1893 
   Size:        251    1642
 
HindIII A'AGCT_T

Cuts at:      0      52     262     397    1893 
   Size:         52     210     135    1496

  Fragments arranged by size: 

                1496     210     135      52
 
KpnI G_GTAC'C

Cuts at:      0    1806    1893 
   Size:       1806      87
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1089    1745    1893 
   Size:       1089     656     148

  Fragments arranged by size: 

                1089     656     148
 
MscI TGG'CCA

Cuts at:      0    1888    1893 
   Size:       1888       5
 
MslI CAynn'nnrTG

Cuts at:      0     228     335    1893 
   Size:        228     107    1558

  Fragments arranged by size: 

                1558     228     107
 
MspA1I CmG'CkG

Cuts at:      0       7      36     301     558     705    1032    1212    1221 
   Size:          7      29     265     257     147     327     180       9

Cuts at:   1221    1231    1306    1322    1359    1690    1758    1893 
   Size:         10      75      16      37     331      68     135

  Fragments arranged by size: 

                 331     327     265     257     180     147     135      75
                  68      37      29      16      10       9       7
 
NaeI GCC'GGC

Cuts at:      0     324     631    1893 
   Size:        324     307    1262

  Fragments arranged by size: 

                1262     324     307
 
NgoMIV G'CCGG_C

Cuts at:      0     322     629    1893 
   Size:        322     307    1264

  Fragments arranged by size: 

                1264     322     307
 
NheI G'CTAG_C

Cuts at:      0     219    1893 
   Size:        219    1674
 
NspI r_CATG'y

Cuts at:      0    1002    1893 
   Size:       1002     891
 
PasI CC'CwG_GG

Cuts at:      0    1095    1893 
   Size:       1095     798
 
PfoI T'CCnGG_A

Cuts at:      0     641     725    1559    1893 
   Size:        641      84     834     334

  Fragments arranged by size: 

                 834     641     334      84
 
PpuMI rG'GwC_Cy

Cuts at:      0     792    1143    1893 
   Size:        792     351     750

  Fragments arranged by size: 

                 792     750     351
 
PspOMI G'GGCC_C

Cuts at:      0     242     707    1669    1893 
   Size:        242     465     962     224

  Fragments arranged by size: 

                 962     465     242     224
 
PstI C_TGCA'G

Cuts at:      0      41     300     464     875    1217    1689    1844    1893 
   Size:         41     259     164     411     342     472     155      49

  Fragments arranged by size: 

                 472     411     342     259     164     155      49      41
            
 
PvuI CG_AT'CG

Cuts at:      0     851    1893 
   Size:        851    1042
 
PvuII CAG'CTG

Cuts at:      0     301    1212    1221    1306    1690    1758    1893 
   Size:        301     911       9      85     384      68     135

  Fragments arranged by size: 

                 911     384     301     135      85      68       9
 
SacI G_AGCT'C

Cuts at:      0    1436    1547    1893 
   Size:       1436     111     346

  Fragments arranged by size: 

                1436     346     111
 
SacII CC_GC'GG

Cuts at:      0    1033    1232    1893 
   Size:       1033     199     661

  Fragments arranged by size: 

                1033     661     199
 
SalI G'TCGA_C

Cuts at:      0     249    1893 
   Size:        249    1644
 
SfcI C'TryA_G

Cuts at:      0      37     296     460     553     871    1213    1437    1685 
   Size:         37     259     164      93     318     342     224     248

Cuts at:   1685    1840    1893 
   Size:        155      53

  Fragments arranged by size: 

                 342     318     259     248     224     164     155      93
                  53      37
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      10    1507    1893 
   Size:         10    1497     386

  Fragments arranged by size: 

                1497     386      10
 
SmaI CCC'GGG

Cuts at:      0      97     595    1405    1876    1893 
   Size:         97     498     810     471      17

  Fragments arranged by size: 

                 810     498     471      97      17
 
SmlI C'TyrA_G

Cuts at:      0     775    1893 
   Size:        775    1118
 
SphI G_CATG'C

Cuts at:      0    1002    1893 
   Size:       1002     891
 
StuI AGG'CCT

Cuts at:      0     454    1295    1883    1893 
   Size:        454     841     588      10

  Fragments arranged by size: 

                 841     588     454      10
 
StyI C'CwwG_G

Cuts at:      0    1313    1502    1893 
   Size:       1313     189     391

  Fragments arranged by size: 

                1313     391     189
 
TatI w'GTAC_w

Cuts at:      0     884    1382    1893 
   Size:        884     498     511

  Fragments arranged by size: 

                 884     511     498
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     121     153    1602    1634    1893 
   Size:        121      32    1449      32     259

  Fragments arranged by size: 

                1449     259     121      32      32
 
XmaI C'CCGG_G

Cuts at:      0      95     593    1403    1874    1893 
   Size:         95     498     810     471      19

  Fragments arranged by size: 

                 810     498     471      95      19
 
ZraI GAC'GTC

Cuts at:      0    1166    1893 
   Size:       1166     727
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     EaeI     EagI  EcoICRI
  Eco57MI EcoO109I    HaeII    Hin4I   HincII  HindIII     KpnI     MmeI
     MscI     MslI   MspA1I     NaeI   NgoMIV     NheI     NspI     PasI
     PfoI    PpuMI   PspOMI     PstI     PvuI    PvuII     SacI    SacII
     SalI     SfcI     SfiI     SmaI     SmlI     SphI     StuI     StyI
     TatI     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AclI     AfeI    AflII   AflIII     AgeI     AlfI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BclI    BfrBI
    BmgBI    BsaBI    BsaWI     BsgI    BsiWI    BsmBI    BspEI    BspHI
   BssHII   BstAPI   BstEII     ClaI     DraI   DraIII     DrdI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
     HpaI     KasI     MfeI     MluI     NarI     NcoI     NdeI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI    RsrII    SanDI     SapI     SbfI
     ScaI    SexAI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmnI
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