Restriction map for pF1KB7340
 (Linear) (Six-base) MAPSORT of: KIBB7340.seq  Check: 7702  from: 1  to: 3468



KIBB7340.orf

 With 182 enzymes: SgfI * 

                        August 20, 2009 16:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1068    3468 
   Size:       1068    2400
 
AccI GT'mk_AC

Cuts at:      0     635    1712    1856    2512    3468 
   Size:        635    1077     144     656     956

  Fragments arranged by size: 

                1077     956     656     635     144
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     585    1739    2777    3468 
   Size:        161     424    1154    1038     691

  Fragments arranged by size: 

                1154    1038     691     424     161
 
AfeI AGC'GCT

Cuts at:      0     942    3468 
   Size:        942    2526
 
AflIII A'CryG_T

Cuts at:      0    3447    3468 
   Size:       3447      21
 
AleI CACnn'nnGTG

Cuts at:      0    2041    2416    3468 
   Size:       2041     375    1052

  Fragments arranged by size: 

                2041    1052     375
 
AlwNI CAG_nnn'CTG

Cuts at:      0     974    1991    2805    3468 
   Size:        974    1017     814     663

  Fragments arranged by size: 

                1017     974     814     663
 
ApaI G_GGCC'C

Cuts at:      0     459     470    2328    3468 
   Size:        459      11    1858    1140

  Fragments arranged by size: 

                1858    1140     459      11
 
ApoI r'AATT_y

Cuts at:      0      19    1644    1803    1942    2317    3288    3394    3468 
   Size:         19    1625     159     139     375     971     106      74

  Fragments arranged by size: 

                1625     971     375     159     139     106      74      19
            
 
AvaI C'yCGr_G

Cuts at:      0      28      86     224     315    1839    3468 
   Size:         28      58     138      91    1524    1629

  Fragments arranged by size: 

                1629    1524     138      91      58      28
 
AvrII C'CTAG_G

Cuts at:      0     867    3468 
   Size:        867    2601
 
BamHI G'GATC_C

Cuts at:      0     344     492    2680    3468 
   Size:        344     148    2188     788

  Fragments arranged by size: 

                2188     788     344     148
 
BanI G'GyrC_C

Cuts at:      0      32      67     577    1835    2262    3316    3468 
   Size:         32      35     510    1258     427    1054     152

  Fragments arranged by size: 

                1258    1054     510     427     152      35      32
 
BanII G_rGCy'C

Cuts at:      0     114     459     470     694    1117    1795    2328    3468 
   Size:        114     345      11     224     423     678     533    1140

  Fragments arranged by size: 

                1140     678     533     423     345     224     114      11
            
 
BbeI G_GCGC'C

Cuts at:      0      71    3468 
   Size:         71    3397
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     292    3468 
   Size:        292    3176
 
BbvCI CC'TCA_GC

Cuts at:      0    1934    2601    3468 
   Size:       1934     667     867

  Fragments arranged by size: 

                1934     867     667
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     177     211     981    1015    3468 
   Size:        177      34     770      34    2453

  Fragments arranged by size: 

                2453     770     177      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1728    3468 
   Size:       1728    1740
 
BclI T'GATC_A

Cuts at:      0    1422    3468 
   Size:       1422    2046
 
BfrBI ATG'CAT

Cuts at:      0    2100    3468 
   Size:       2100    1368
 
BlpI GC'TnA_GC

Cuts at:      0    1064    2882    3468 
   Size:       1064    1818     586

  Fragments arranged by size: 

                1818    1064     586
 
Bme1580I G_kGCm'C

Cuts at:      0     459     470     580     747    2265    2328    2367    2869 
   Size:        459      11     110     167    1518      63      39     502

Cuts at:   2869    3468 
   Size:        599

  Fragments arranged by size: 

                1518     599     502     459     167     110      63      39
                  11
 
BmgBI CAC'GTC

Cuts at:      0     591    1652    3468 
   Size:        591    1061    1816

  Fragments arranged by size: 

                1816    1061     591
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     326    1979    2354    2966    3080    3468 
   Size:        326    1653     375     612     114     388

  Fragments arranged by size: 

                1653     612     388     375     326     114
 
BmtI G_CTAG'C

Cuts at:      0    3221    3468 
   Size:       3221     247
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     962    3468 
   Size:        962    2506
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     457     643     668    1767    2009    2384    2508    2993 
   Size:        457     186      25    1099     242     375     124     485

Cuts at:   2993    3468 
   Size:        475

  Fragments arranged by size: 

                1099     485     475     457     375     242     186     124
                  25
 
Bpu10I CC'TnA_GC

Cuts at:      0     538     788    1777    1934    2002    2377    2601    3468 
   Size:        538     250     989     157      68     375     224     867

  Fragments arranged by size: 

                 989     867     538     375     250     224     157      68
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     436    1085    1153    1263    2637    3468 
   Size:        436     649      68     110    1374     831

  Fragments arranged by size: 

                1374     831     649     436     110      68
 
BsaAI yAC'GTr

Cuts at:      0     391    3468 
   Size:        391    3077
 
BsaBI GATnn'nnATC

Cuts at:      0     491    3468 
   Size:        491    2977
 
BsaHI Gr'CG_yC

Cuts at:      0      68      92     679    3468 
   Size:         68      24     587    2789

  Fragments arranged by size: 

                2789     587      68      24
 
BsaWI w'CCGG_w

Cuts at:      0     495     768     892    3468 
   Size:        495     273     124    2576

  Fragments arranged by size: 

                2576     495     273     124
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     580     610    2094    2124    2469    2499    3468 
   Size:        580      30    1484      30     345      30     969

  Fragments arranged by size: 

                1484     969     580     345      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     341     454     938    1373    1562    1565    2012    2099 
   Size:        341     113     484     435     189       3     447      87

Cuts at:   2099    2548    3468 
   Size:        449     920

  Fragments arranged by size: 

                 920     484     449     447     435     341     189     113
                  87       3
 
BseYI C'CCAG_C

Cuts at:      0     355     423     718     859    1184    2203    2763    3136 
   Size:        355      68     295     141     325    1019     560     373

Cuts at:   3136    3468 
   Size:        332

  Fragments arranged by size: 

                1019     560     373     355     332     325     295     141
                  68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1234    1844    2231    2612    3188    3468 
   Size:        520     714     610     387     381     576     280

  Fragments arranged by size: 

                 714     610     576     520     387     381     280
 
BsiEI CG_ry'CG

Cuts at:      0     108     236    3468 
   Size:        108     128    3232

  Fragments arranged by size: 

                3232     128     108
 
BsiHKAI G_wGCw'C

Cuts at:      0     694    1795    3468 
   Size:        694    1101    1673

  Fragments arranged by size: 

                1673    1101     694
 
BsmI GAATG_Cn'

Cuts at:      0    1363    3468 
   Size:       1363    2105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     581     686    3468 
   Size:        581     105    2782

  Fragments arranged by size: 

                2782     581     105
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     459     470     580     694     747    1117    1795 
   Size:        114     345      11     110     114      53     370     678

Cuts at:   1795    2265    2328    2367    2869    3468 
   Size:        470      63      39     502     599

  Fragments arranged by size: 

                 678     599     502     470     370     345     114     114
                 110      63      53      39      11
 
BspEI T'CCGG_A

Cuts at:      0     768     892    3468 
   Size:        768     124    2576

  Fragments arranged by size: 

                2576     768     124
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1068    3468 
   Size:       1068    2400
 
BsrBI CCG'CTC

Cuts at:      0      76    3468 
   Size:         76    3392
 
BsrDI GCAATG_nn'

Cuts at:      0     997    1027    1815    2594    3468 
   Size:        997      30     788     779     874

  Fragments arranged by size: 

                 997     874     788     779      30
 
BsrFI r'CCGG_y

Cuts at:      0      41     255     396    3468 
   Size:         41     214     141    3072

  Fragments arranged by size: 

                3072     214     141      41
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     435    1034    1915    3421    3468 
   Size:        435     599     881    1506      47

  Fragments arranged by size: 

                1506     881     599     435      47
 
BstYI r'GATC_y

Cuts at:      0     344     492    2680    3322    3468 
   Size:        344     148    2188     642     146

  Fragments arranged by size: 

                2188     642     344     148     146
 
BtgI C'CryG_G

Cuts at:      0     141    3468 
   Size:        141    3327
 
BtsI GCAGTG_nn'

Cuts at:      0    1021    1827    2213    2430    3303    3468 
   Size:       1021     806     386     217     873     165

  Fragments arranged by size: 

                1021     873     806     386     217     165
 
DraI TTT'AAA

Cuts at:      0    2291    2672    3468 
   Size:       2291     381     796

  Fragments arranged by size: 

                2291     796     381
 
DraIII CAC_nnn'GTG

Cuts at:      0     391    2809    3468 
   Size:        391    2418     659

  Fragments arranged by size: 

                2418     659     391
 
EaeI y'GGCC_r

Cuts at:      0     233     275    3186    3468 
   Size:        233      42    2911     282

  Fragments arranged by size: 

                2911     282     233      42
 
EagI C'GGCC_G

Cuts at:      0     233    3468 
   Size:        233    3235
 
EarI CTCTTCn'nnn_

Cuts at:      0     468     842    1105    1397    1598    3468 
   Size:        468     374     263     292     201    1870

  Fragments arranged by size: 

                1870     468     374     292     263     201
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     142    3468 
   Size:        142    3326
 
EcoICRI GAG'CTC

Cuts at:      0     692    1793    3468 
   Size:        692    1101    1675

  Fragments arranged by size: 

                1675    1101     692
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     457     585     643     668    1739    1767    2009 
   Size:        161     296     128      58      25    1071      28     242

Cuts at:   2009    2384    2508    2777    2993    3468 
   Size:        375     124     269     216     475

  Fragments arranged by size: 

                1071     475     375     296     269     242     216     161
                 128     124      58      28      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     978    3468 
   Size:        978    2490
 
EcoO109I rG'GnC_Cy

Cuts at:      0     249     466     530     569     706     971    1616    2324 
   Size:        249     217      64      39     137     265     645     708

Cuts at:   2324    2325    3028    3082    3177    3337    3468 
   Size:          1     703      54      95     160     131

  Fragments arranged by size: 

                 708     703     645     265     249     217     160     137
                 131      95      64      54      39       1
 
EcoRI G'AATT_C

Cuts at:      0    1644    3288    3468 
   Size:       1644    1644     180

  Fragments arranged by size: 

                1644    1644     180
 
HaeII r_GCGC'y

Cuts at:      0      71     784     944    1474    3468 
   Size:         71     713     160     530    1994

  Fragments arranged by size: 

                1994     713     530     160      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     198     230     336     368     580     612    1295    1327 
   Size:        198      32     106      32     212      32     683      32

Cuts at:   1327    2830    2862    3468 
   Size:       1503      32     606

  Fragments arranged by size: 

                1503     683     606     212     198     106      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    2843    3468 
   Size:       2843     625
 
HindIII A'AGCT_T

Cuts at:      0    1973    3468 
   Size:       1973    1495
 
HpaI GTT'AAC

Cuts at:      0    2843    3468 
   Size:       2843     625
 
KasI G'GCGC_C

Cuts at:      0      67    3468 
   Size:         67    3401
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2303    2973    3312    3468 
   Size:       2303     670     339     156

  Fragments arranged by size: 

                2303     670     339     156
 
MscI TGG'CCA

Cuts at:      0     277    3188    3468 
   Size:        277    2911     280

  Fragments arranged by size: 

                2911     280     277
 
MslI CAynn'nnrTG

Cuts at:      0    1032    1820    2041    2416    2959    3419    3468 
   Size:       1032     788     221     375     543     460      49

  Fragments arranged by size: 

                1032     788     543     460     375     221      49
 
MspA1I CmG'CkG

Cuts at:      0     143     545     885    1011    2207    3468 
   Size:        143     402     340     126    1196    1261

  Fragments arranged by size: 

                1261    1196     402     340     143     126
 
NaeI GCC'GGC

Cuts at:      0      43     257     398    3468 
   Size:         43     214     141    3070

  Fragments arranged by size: 

                3070     214     141      43
 
NarI GG'CG_CC

Cuts at:      0      68    3468 
   Size:         68    3400
 
NdeI CA'TA_TG

Cuts at:      0    3435    3468 
   Size:       3435      33
 
NgoMIV G'CCGG_C

Cuts at:      0      41     255     396    3468 
   Size:         41     214     141    3072

  Fragments arranged by size: 

                3072     214     141      41
 
NheI G'CTAG_C

Cuts at:      0    3217    3468 
   Size:       3217     251
 
NotI GC'GGCC_GC

Cuts at:      0     233    3468 
   Size:        233    3235
 
NsiI A_TGCA'T

Cuts at:      0    2102    3468 
   Size:       2102    1366
 
NspI r_CATG'y

Cuts at:      0     366    3072    3451    3468 
   Size:        366    2706     379      17

  Fragments arranged by size: 

                2706     379     366      17
 
PasI CC'CwG_GG

Cuts at:      0     573     582     613    3468 
   Size:        573       9      31    2855

  Fragments arranged by size: 

                2855     573      31       9
 
PciI A'CATG_T

Cuts at:      0    3447    3468 
   Size:       3447      21
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      64     799    2809    3195    3468 
   Size:         64     735    2010     386     273

  Fragments arranged by size: 

                2010     735     386     273      64
 
PfoI T'CCnGG_A

Cuts at:      0     434    2295    2524    3468 
   Size:        434    1861     229     944

  Fragments arranged by size: 

                1861     944     434     229
 
PmlI CAC'GTG

Cuts at:      0     391    3468 
   Size:        391    3077
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2038    2070    3468 
   Size:       2038      32    1398

  Fragments arranged by size: 

                2038    1398      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     249     530     569    1616    3028    3177    3468 
   Size:        249     281      39    1047    1412     149     291

  Fragments arranged by size: 

                1412    1047     291     281     249     149      39
 
PshAI GACnn'nnGTC

Cuts at:      0    1852    3468 
   Size:       1852    1616
 
PsiI TTA'TAA

Cuts at:      0    1451    3468 
   Size:       1451    2017
 
PspOMI G'GGCC_C

Cuts at:      0     455     466    2324    3468 
   Size:        455      11    1858    1144

  Fragments arranged by size: 

                1858    1144     455      11
 
PstI C_TGCA'G

Cuts at:      0     818    2436    3374    3468 
   Size:        818    1618     938      94

  Fragments arranged by size: 

                1618     938     818      94
 
SacI G_AGCT'C

Cuts at:      0     694    1795    3468 
   Size:        694    1101    1673

  Fragments arranged by size: 

                1673    1101     694
 
SacII CC_GC'GG

Cuts at:      0     144    3468 
   Size:        144    3324
 
SanDI GG'GwC_CC

Cuts at:      0    3177    3468 
   Size:       3177     291
 
SapI GCTCTTCn'nnn_

Cuts at:      0     842    1105    1397    3468 
   Size:        842     263     292    2071

  Fragments arranged by size: 

                2071     842     292     263
 
SexAI A'CCwGG_T

Cuts at:      0    2826    3468 
   Size:       2826     642
 
SfcI C'TryA_G

Cuts at:      0     814    2057    2270    2432    3370    3468 
   Size:        814    1243     213     162     938      98

  Fragments arranged by size: 

                1243     938     814     213     162      98
 
SfoI GGC'GCC

Cuts at:      0      69    3468 
   Size:         69    3399
 
SmaI CCC'GGG

Cuts at:      0      30      88    1841    3468 
   Size:         30      58    1753    1627

  Fragments arranged by size: 

                1753    1627      58      30
 
StuI AGG'CCT

Cuts at:      0     174    3468 
   Size:        174    3294
 
StyI C'CwwG_G

Cuts at:      0     867     934    1103    2117    2226    3468 
   Size:        867      67     169    1014     109    1242

  Fragments arranged by size: 

                1242    1014     867     169     109      67
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     306     460     595    1198    1408    3150    3427    3468 
   Size:        306     154     135     603     210    1742     277      41

  Fragments arranged by size: 

                1742     603     306     277     210     154     135      41
            
 
TatI w'GTAC_w

Cuts at:      0    2537    3468 
   Size:       2537     931
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351    2607    2639    3468 
   Size:        319      32    2256      32     829

  Fragments arranged by size: 

                2256     829     319      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1281    1344    2806    3468 
   Size:       1281      63    1462     662

  Fragments arranged by size: 

                1462    1281     662      63
 
XmaI C'CCGG_G

Cuts at:      0      28      86    1839    3468 
   Size:         28      58    1753    1629

  Fragments arranged by size: 

                1753    1629      58      28
 
XmnI GAAnn'nnTTC

Cuts at:      0     919    3468 
   Size:        919    2549
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI   AflIII     AleI    AlwNI     ApaI
     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BstXI    BstYI     BtgI     BtsI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NsiI
     NspI     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PstI     SacI    SacII    SanDI     SapI
    SexAI     SfcI     SfoI     SmaI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII     AgeI     AhdI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI     BaeI     BglI    BglII    BpuEI
    BsiWI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI    EcoRV     FalI
     FseI     FspI    FspAI     KpnI     MfeI     MluI     NcoI     NruI
     PacI     PmeI    PspXI     PsrI     PvuI    PvuII    RsrII     SalI
     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XhoI     ZraI
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