Restriction map for pF1KE0210
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BglI        BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AflII         637  CTTAAG               C^TTAAG
Aor51HI       294  AGCGCT               AGC^GCT
AvaII         375  GGACC                G^G[AT]CC
BalI          317  TGGCCA               TGG^CCA
BbvCI         306  GCTGAGG              CCTCAGC(-5/-2)
BcnI          358  CCCGG                CC^[GC]GG
BfrI          637  CTTAAG               C^TTAAG
BglII         667  AGATCT               A^GATCT
BmyI          339  GTGCCC               G[AGT]GC[ACT]^C
BpmI          165  CTCCAG               CTGGAG(16/14)
BseRI         362  GAGGAG               GAGGAG(10/8)
BsmBI         367  GAGACG               CGTCTC(1/5)
Bsp1286I      339  GTGCCC               G[AGT]GC[ACT]^C
BspHI         176  TCATGA               T^CATGA
BstF5I        701  GGATG                GGATG(2/0)
Cac8I           4  GCTCGC               GC[ACGT]^[ACGT]GC
CfoI          295  GCGC                 GCG^C
DraI          106  TTTAAA               TTT^AAA
DsaI          314  CCATGG               C^C[GA][CT]GG
EaeI          317  TGGCCA               [CT]^GGCC[GA]
Eco47III      294  AGCGCT               AGC^GCT
EcoT22I       196  ATGCAT               ATGCA^T
FokI          701  GGATG                GGATG(9/13)
HaeII         294  AGCGCT               [GA]GCGC^[CT]
HgaI          369  GACGC                GACGC(5/10)
HhaI          295  GCGC                 GCG^C
HinP1I        295  GCGC                 G^CGC
HincII        419  GTCAAC               GT[CT]^[GA]AC
HindII        419  GTCAAC               GT[CT]^[GA]AC
HphI          381  GGTGA                GGTGA(8/7)
MluNI         317  TGGCCA               TGG^CCA
MscI          317  TGGCCA               TGG^CCA
MslI          784  CATCTAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          358  CCCGG                CC^[GC]GG
NcoI          314  CCATGG               C^CATGG
NsiI          196  ATGCAT               ATGCA^T
PshAI          18  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         119  CAGCTG               CAG^CTG
RcaI          176  TCATGA               T^CATGA
SexAI         377  ACCAGGT              A^CC[AT]GGT
SfcI          775  CTACAG               C^T[GA][CT]AG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BsmAI         367  GAGAC                GTCTC(1/5)
              707  GTCTC               
BstYI         466  GGATCT               [GA]^GATC[CT]
              667  AGATCT              
Cfr13I        311  GGGCC                G^G[ACGT]CC
              375  GGACC               
EarI          334  GAAGAG               CTCTTC(1/4)
              734  GAAGAG              
HaeIII        312  GGCC                 GG^CC
              318  GGCC                
HapII          27  CCGG                 C^CGG
              359  CCGG                
HpaII          27  CCGG                 C^CGG
              359  CCGG                
Ksp632I       334  GAAGAG               CTCTTC(1/4)
              734  GAAGAG              
MaeIII        590  GTCAC                ^GT[ACGT]AC
              685  GTCAC               
MboII         334  GAAGA                GAAGA(8/7)
              734  GAAGA               
MflI          466  GGATCT               [GA]^GATC[CT]
              667  AGATCT              
MspA1I        119  CAGCTG               C[AC]G^C[GT]G
              549  CAGCGG              
MspI           27  CCGG                 C^CGG
              359  CCGG                
Sau96I        311  GGGCC                G^G[ACGT]CC
              375  GGACC               
SmlI          252  CTCAAG               C^T[CT][GA]AG
              637  CTTAAG              
Tsp45I        590  GTCAC                ^GT[GC]AC
              685  GTCAC               
XhoII         466  GGATCT               [GA]^GATC[CT]
              667  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        AlwNI       
ApoI        BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstAPI      BstNI       DdeI        
DpnII       EcoRII      EcoT14I     Fnu4HI      HinfI       
ItaI        MboI        MnlI        MseI        MvaI        
MwoI        NdeII       NlaIII      PleI        Sau3AI      
ScrFI       StyI        Tru9I       TseI        Tsp509I     
TspRI       
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