Restriction map for
pF1KB5394
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MluNI MscI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AcsI 147 GAATTT [GA]^AATT[CT]
AcyI 1744 GGCGTC G[GA]^CG[CT]C
AhdI 403 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1306 AGCGCT AGC^GCT
ApoI 147 GAATTT [GA]^AATT[CT]
AspEI 403 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 622 GAGCTC G[AT]GC[AT]^C
BbsI 1747 GTCTTC GAAGAC(2/6)
BbvCI 1074 CCTCAGC CCTCAGC(-5/-2)
BcgI 1375 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1747 GTCTTC GAAGAC(2/6)
BsaAI 381 CACGTA [CT]AC^GT[GA]
BsaHI 1744 GGCGTC G[GA]^CG[CT]C
BsgI 52 GTGCAG GTGCAG(16/14)
BsiHKAI 622 GAGCTC G[AT]GC[AT]^C
BsmBI 1435 GAGACG CGTCTC(1/5)
BsrDI 417 CATTGC GCAATG(2/0)
BssHII 71 GCGCGC G^CGCGC
BstXI 1163 CCAGTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1090 GACACGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 403 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1306 AGCGCT AGC^GCT
Hin1I 1744 GGCGTC G[GA]^CG[CT]C
MslI 1689 CATGGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 160 CAGCTG C[AC]G^C[GT]G
PvuII 160 CAGCTG CAG^CTG
SacI 622 GAGCTC GAGCT^C
SapI 1666 GAAGAGC GCTCTTC(1/4)
SfaNI 731 GCATC GCATC(5/9)
SfiI 954 GGCCGGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1379 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 194 TCCGGA T^CCGGA
1004 TCCGGA
AfaI 384 GTAC GT^AC
1573 GTAC
BglI 273 GCCCGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
955 GCCGGCGGGGC
BsaI 112 GAGACC GGTCTC(1/5)
1486 GAGACC
BsaWI 194 TCCGGA [AT]^CCGG[AT]
1004 TCCGGA
BseAI 194 TCCGGA T^CCGGA
1004 TCCGGA
BsiEI 953 CGGCCG CG[GA][CT]^CG
1349 CGGCCG
BspEI 194 TCCGGA T^CCGGA
1004 TCCGGA
BssSI 226 CACGAG CACGAG(-5/-1)
940 CACGAG
EagI 953 CGGCCG C^GGCCG
1349 CGGCCG
EarI 1466 CTCTTC CTCTTC(1/4)
1666 GAAGAG
EclXI 953 CGGCCG C^GGCCG
1349 CGGCCG
Eco52I 953 CGGCCG C^GGCCG
1349 CGGCCG
EcoNI 868 CCTGCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1080 CCTGTTTGAGG
HgaI 140 GCGTC GACGC(5/10)
1745 GCGTC
HincII 715 GTCAAC GT[CT]^[GA]AC
934 GTCAAC
HindII 715 GTCAAC GT[CT]^[GA]AC
934 GTCAAC
Ksp632I 1466 CTCTTC CTCTTC(1/4)
1666 GAAGAG
MroI 194 TCCGGA T^CCGGA
1004 TCCGGA
NcoI 374 CCATGG C^CATGG
1246 CCATGG
PflMI 90 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
345 CCAGAAGCTGG
RsaI 384 GTAC GT^AC
1573 GTAC
SmaI 970 CCCGGG CCC^GGG
1140 CCCGGG
SmlI 532 CTTGAG C^T[CT][GA]AG
1621 CTCAAG
Van91I 90 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
345 CCAGAAGCTGG
XcmI 1246 CCATGGGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1404 CCAACCTGCGGCTGG
XmaCI 970 CCCGGG C^CCGGG
1140 CCCGGG
XmaI 970 CCCGGG C^CCGGG
1140 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI Sau3AI Sau96I
ScrFI SfcI StyI TseI Tsp45I
Tsp509I TspRI XhoII