Restriction map for pF1KB6777
 (Circular) (Six-base) MAPSORT of: pF1KB6777.seq  Check: 8862  from: 1  to: 3470



pF1KB6777  3470 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    508     508 
   Size:       3470
 
Acc65I G'GTAC_C

Cuts at:    486     486 
   Size:       3470
 
AclI AA'CG_TT

Cuts at:   3310    3310 
   Size:       3470
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    307    1295    1727    2474    2742     307 
   Size:        988     432     747     268    1035

  Fragments arranged by size: 

                1035     988     747     432     268
 
AflIII A'CryG_T

Cuts at:    822    1926    2903     822 
   Size:       1104     977    1389

  Fragments arranged by size: 

                1389    1104     977
 
AgeI A'CCGG_T

Cuts at:   1858    1858 
   Size:       3470
 
AlwNI CAG_nnn'CTG

Cuts at:    775    2342     775 
   Size:       1567    1903
 
ApaLI G'TGCA_C

Cuts at:   2240    2240 
   Size:       3470
 
ApoI r'AATT_y

Cuts at:    228     373     474    2823    3079     228 
   Size:        145     101    2349     256     619

  Fragments arranged by size: 

                2349     619     256     145     101
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3470
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3470
 
AvaI C'yCGr_G

Cuts at:    490     490 
   Size:       3470
 
BamHI G'GATC_C

Cuts at:    495     495 
   Size:       3470
 
BanI G'GyrC_C

Cuts at:    486     900    1148    1183     486 
   Size:        414     248      35    2773

  Fragments arranged by size: 

                2773     414     248      35
 
BanII G_rGCy'C

Cuts at:    484    1514     484 
   Size:       1030    2440
 
BbeI G_GCGC'C

Cuts at:    904    1152     904 
   Size:        248    3222
 
BciVI GTATCCnnnnn_n'

Cuts at:   1368    2129    3470    1368 
   Size:        761    1341    1368

  Fragments arranged by size: 

                1368    1341     761
 
BclI T'GATC_A

Cuts at:    990     990 
   Size:       3470
 
BglII A'GATC_T

Cuts at:    985    3022     985 
   Size:       2037    1433
 
BlpI GC'TnA_GC

Cuts at:    582     582 
   Size:       3470
 
Bme1580I G_kGCm'C

Cuts at:   1095    1188    2244    1095 
   Size:         93    1056    2321

  Fragments arranged by size: 

                2321    1056      93
 
BmgBI CAC'GTC

Cuts at:    202     202 
   Size:       3470
 
BmrI ACTGGGnnnn_n'

Cuts at:    682     763    1098    3242     682 
   Size:         81     335    2144     910

  Fragments arranged by size: 

                2144     910     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1837    1837 
   Size:       3470
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     181 
   Size:       3470
 
Bpu10I CC'TnA_GC

Cuts at:    126     126 
   Size:       3470
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    646    1525    2017    2315     646 
   Size:        879     492     298    1801

  Fragments arranged by size: 

                1801     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3174    3174 
   Size:       3470
 
BsaAI yAC'GTr

Cuts at:    823     823 
   Size:       3470
 
BsaBI GATnn'nnATC

Cuts at:      6     984    3021       6 
   Size:        978    2037     455

  Fragments arranged by size: 

                2037     978     455
 
BsaHI Gr'CG_yC

Cuts at:    901    1149    1851    3347     901 
   Size:        248     702    1496    1024

  Fragments arranged by size: 

                1496    1024     702     248
 
BsaWI w'CCGG_w

Cuts at:    659    1180    1858    2132    2279    2690     659 
   Size:        521     678     274     147     411    1439

  Fragments arranged by size: 

                1439     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    895    1653    2230    2726     895 
   Size:        758     577     496    1639

  Fragments arranged by size: 

                1639     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1012    1058    2266     104 
   Size:        908      46    1208    1308

  Fragments arranged by size: 

                1308    1208     908      46
 
BsiHKAI G_wGCw'C

Cuts at:    137     484    1262    1452    2244     137 
   Size:        347     778     190     792    1363

  Fragments arranged by size: 

                1363     792     778     347     190
 
BsmI GAATG_Cn'

Cuts at:    318     318 
   Size:       3470
 
Bsp1286I G_dGCh'C

Cuts at:    137     484    1095    1188    1262    1452    1514    2244     137 
   Size:        347     611      93      74     190      62     730    1363

  Fragments arranged by size: 

                1363     730     611     347     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2690    2690 
   Size:       3470
 
BspHI T'CATG_A

Cuts at:   2646    2646 
   Size:       3470
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    520     734    1036    1417     520 
   Size:        214     302     381    2573

  Fragments arranged by size: 

                2573     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1762    1816    3300    3465      50 
   Size:       1712      54    1484     165      55

  Fragments arranged by size: 

                1712    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1382    1382 
   Size:       3470
 
BsrFI r'CCGG_y

Cuts at:   1468    1649    1858    1468 
   Size:        181     209    3080

  Fragments arranged by size: 

                3080     209     181
 
BssSI C'ACGA_G

Cuts at:   1741    2099    1741 
   Size:        358    3112
 
BstAPI GCAn_nnn'nTGC

Cuts at:    847     847 
   Size:       3470
 
BstBI TT'CG_AA

Cuts at:   1831    1831 
   Size:       3470
 
BstYI r'GATC_y

Cuts at:      1     495     962     985    1320    1566    2578    2664    2676 
   Size:        494     467      23     335     246    1012      86      12

Cuts at:   2676    3022       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     494     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    119     119 
   Size:       3470
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1420    1601    1764    1420 
   Size:        181     163    3126

  Fragments arranged by size: 

                3126     181     163
 
BtsI GCAGTG_nn'

Cuts at:    713     713 
   Size:       3470
 
DraI TTT'AAA

Cuts at:    469     469 
   Size:       3470
 
DrdI GACnn_nn'nnGTC

Cuts at:   1176    2034    1176 
   Size:        858    2612
 
EaeI y'GGCC_r

Cuts at:   1055    1229    1620    1647    2992    1055 
   Size:        174     391      27    1345    1533

  Fragments arranged by size: 

                1533    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1055    1055 
   Size:       3470
 
EarI CTCTTCn'nnn_

Cuts at:   1493    1703    1493 
   Size:        210    3260
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1678    1988    2134    3280    1678 
   Size:        310     146    1146    1868

  Fragments arranged by size: 

                1868    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    482     482 
   Size:       3470
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    181     307    1295    1727    2474    2742     181 
   Size:        126     988     432     747     268     909

  Fragments arranged by size: 

                 988     909     747     432     268     126
 
EcoO109I rG'GnC_Cy

Cuts at:    609     609 
   Size:       3470
 
EcoRI G'AATT_C

Cuts at:    373     474     373 
   Size:        101    3369
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1489    1489 
   Size:       3470
 
FspI TGC'GCA

Cuts at:   1251    1251 
   Size:       3470
 
HaeII r_GCGC'y

Cuts at:    904    1152    2174    3153     904 
   Size:        248    1022     979    1221

  Fragments arranged by size: 

                1221    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1312    1342    1344    1374    1312 
   Size:         30       2      30    3408

  Fragments arranged by size: 

                3408      30      30       2
 
HincII GTy'rAC

Cuts at:    279     509     279 
   Size:        230    3240
 
KasI G'GCGC_C

Cuts at:    900    1148     900 
   Size:        248    3222
 
KpnI G_GTAC'C

Cuts at:    490     490 
   Size:       3470
 
MluI A'CGCG_T

Cuts at:   2903    2903 
   Size:       3470
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    290    2141    2325     290 
   Size:       1851     184    1435

  Fragments arranged by size: 

                1851    1435     184
 
MscI TGG'CCA

Cuts at:   1231    1231 
   Size:       3470
 
MslI CAynn'nnrTG

Cuts at:   1459    1586    1459 
   Size:        127    3343
 
MspA1I CmG'CkG

Cuts at:    581     895    1255    2268    2513     581 
   Size:        314     360    1013     245    1538

  Fragments arranged by size: 

                1538    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1651    1651 
   Size:       3470
 
NarI GG'CG_CC

Cuts at:    901    1149     901 
   Size:        248    3222
 
NgoMIV G'CCGG_C

Cuts at:   1649    1649 
   Size:       3470
 
NspI r_CATG'y

Cuts at:    523    1023    1930     523 
   Size:        500     907    2063

  Fragments arranged by size: 

                2063     907     500
 
PciI A'CATG_T

Cuts at:   1926    1926 
   Size:       3470
 
PmeI GTTT'AAAC

Cuts at:    469     469 
   Size:       3470
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2609    2641    2609 
   Size:         32    3438
 
PstI C_TGCA'G

Cuts at:    517    1202     517 
   Size:        685    2785
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3470
 
PvuII CAG'CTG

Cuts at:    895    1255     895 
   Size:        360    3110
 
RsrII CG'GwC_CG

Cuts at:   1665    1665 
   Size:       3470
 
SacI G_AGCT'C

Cuts at:    484     484 
   Size:       3470
 
SalI G'TCGA_C

Cuts at:    507     507 
   Size:       3470
 
SapI GCTCTTCn'nnn_

Cuts at:   1493    1703    1493 
   Size:        210    3260
 
SbfI CC_TGCA'GG

Cuts at:    517     517 
   Size:       3470
 
ScaI AGT'ACT

Cuts at:   2987    2987 
   Size:       3470
 
SfcI C'TryA_G

Cuts at:     33     513    1198    2191    2382      33 
   Size:        480     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     480     191
 
SfoI GGC'GCC

Cuts at:    902    1150     902 
   Size:        248    3222
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3470
 
SmaI CCC'GGG

Cuts at:    492     492 
   Size:       3470
 
SmlI C'TyrA_G

Cuts at:    625    1540    2032    2294     625 
   Size:        915     492     262    1801

  Fragments arranged by size: 

                1801     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3470
 
SphI G_CATG'C

Cuts at:    523    1023     523 
   Size:        500    2970
 
StyI C'CwwG_G

Cuts at:    604     958     604 
   Size:        354    3116
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1053    1186    1645    1854    1053 
   Size:        133     459     209    2669

  Fragments arranged by size: 

                2669     459     209     133
 
TatI w'GTAC_w

Cuts at:    362    2985     362 
   Size:       2623     847
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2987    3019    2987 
   Size:         32    3438
 
Tth111I GACn'n_nGTC

Cuts at:    426    1267     426 
   Size:        841    2629
 
XbaI T'CTAG_A

Cuts at:    501     501 
   Size:       3470
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    965     965 
   Size:       3470
 
XmaI C'CCGG_G

Cuts at:    490     490 
   Size:       3470
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PmeI     PpiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI     BmtI    BsaXI    BseRI     BsgI    BsiWI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI     ZraI
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