Restriction map for pF1KE0424
 (Linear) (Six-base) MAPSORT of: KIEE0424.seq  Check: 8721  from: 1  to: 1410



KIEE0424.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     407    1410 
   Size:        407    1003
 
Acc65I G'GTAC_C

Cuts at:      0     952    1410 
   Size:        952     458
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520    1174    1410 
   Size:        520     654     236

  Fragments arranged by size: 

                 654     520     236
 
AlwNI CAG_nnn'CTG

Cuts at:      0     618    1003    1250    1410 
   Size:        618     385     247     160

  Fragments arranged by size: 

                 618     385     247     160
 
ApaI G_GGCC'C

Cuts at:      0     813    1091    1384    1410 
   Size:        813     278     293      26

  Fragments arranged by size: 

                 813     293     278      26
 
AvaI C'yCGr_G

Cuts at:      0      23     454    1410 
   Size:         23     431     956

  Fragments arranged by size: 

                 956     431      23
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     944     977    1410 
   Size:        944      33     433

  Fragments arranged by size: 

                 944     433      33
 
BamHI G'GATC_C

Cuts at:      0     797    1410 
   Size:        797     613
 
BanI G'GyrC_C

Cuts at:      0     570     952    1410 
   Size:        570     382     458

  Fragments arranged by size: 

                 570     458     382
 
BanII G_rGCy'C

Cuts at:      0     174     813    1091    1384    1410 
   Size:        174     639     278     293      26

  Fragments arranged by size: 

                 639     293     278     174      26
 
BbvCI CC'TCA_GC

Cuts at:      0    1342    1410 
   Size:       1342      68
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     397     431    1410 
   Size:        397      34     979

  Fragments arranged by size: 

                 979     397      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     706    1410 
   Size:        706     704
 
BlpI GC'TnA_GC

Cuts at:      0     289    1410 
   Size:        289    1121
 
Bme1580I G_kGCm'C

Cuts at:      0     813    1091    1384    1410 
   Size:        813     278     293      26

  Fragments arranged by size: 

                 813     293     278      26
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     991    1410 
   Size:        991     419
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1251    1410 
   Size:       1251     159
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265    1410 
   Size:        265    1145
 
Bpu10I CC'TnA_GC

Cuts at:      0     619    1129    1342    1410 
   Size:        619     510     213      68

  Fragments arranged by size: 

                 619     510     213      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613    1333    1410 
   Size:        613     720      77

  Fragments arranged by size: 

                 720     613      77
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     375    1138    1410 
   Size:        375     763     272

  Fragments arranged by size: 

                 763     375     272
 
BseYI C'CCAG_C

Cuts at:      0     249    1090    1410 
   Size:        249     841     320

  Fragments arranged by size: 

                 841     320     249
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     895    1315    1410 
   Size:        895     420      95

  Fragments arranged by size: 

                 895     420      95
 
BsiEI CG_ry'CG

Cuts at:      0     182    1410 
   Size:        182    1228
 
BsiHKAI G_wGCw'C

Cuts at:      0     625    1410 
   Size:        625     785
 
BsmI GAATG_Cn'

Cuts at:      0     369    1410 
   Size:        369    1041
 
Bsp1286I G_dGCh'C

Cuts at:      0     174     625     813    1091    1384    1410 
   Size:        174     451     188     278     293      26

  Fragments arranged by size: 

                 451     293     278     188     174      26
 
BspHI T'CATG_A

Cuts at:      0     189    1410 
   Size:        189    1221
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     120     449     603    1410 
   Size:        120     329     154     807

  Fragments arranged by size: 

                 807     329     154     120
 
BsrBI CCG'CTC

Cuts at:      0     790    1410 
   Size:        790     620
 
BsrDI GCAATG_nn'

Cuts at:      0    1053    1410 
   Size:       1053     357
 
BsrFI r'CCGG_y

Cuts at:      0      78    1410 
   Size:         78    1332
 
BssSI C'ACGA_G

Cuts at:      0    1150    1410 
   Size:       1150     260
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1185    1410 
   Size:       1185     225
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1048    1410 
   Size:       1048     362
 
BstYI r'GATC_y

Cuts at:      0     234     797    1202    1410 
   Size:        234     563     405     208

  Fragments arranged by size: 

                 563     405     234     208
 
BtgI C'CryG_G

Cuts at:      0      11      40      70     701    1022    1355    1410 
   Size:         11      29      30     631     321     333      55

  Fragments arranged by size: 

                 631     333     321      55      30      29      11
 
BtsI GCAGTG_nn'

Cuts at:      0    1107    1410 
   Size:       1107     303
 
EaeI y'GGCC_r

Cuts at:      0       8     179     482     707     980    1410 
   Size:          8     171     303     225     273     430

  Fragments arranged by size: 

                 430     303     273     225     171       8
 
EagI C'GGCC_G

Cuts at:      0     179    1410 
   Size:        179    1231
 
EarI CTCTTCn'nnn_

Cuts at:      0    1315    1410 
   Size:       1315      95
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     136     661    1410 
   Size:        136     525     749

  Fragments arranged by size: 

                 749     525     136
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     520    1174    1410 
   Size:        265     255     654     236

  Fragments arranged by size: 

                 654     265     255     236
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     918    1410 
   Size:        918     492
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     253     615     849    1087    1380    1381    1410 
   Size:        132     121     362     234     238     293       1      29

  Fragments arranged by size: 

                 362     293     238     234     132     121      29       1
            
 
HaeII r_GCGC'y

Cuts at:      0      58     347    1032    1410 
   Size:         58     289     685     378

  Fragments arranged by size: 

                 685     378     289      58
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     750     782    1344    1376    1410 
   Size:        750      32     562      32      34

  Fragments arranged by size: 

                 750     562      34      32      32
 
HincII GTy'rAC

Cuts at:      0     408    1410 
   Size:        408    1002
 
KpnI G_GTAC'C

Cuts at:      0     956    1410 
   Size:        956     454
 
MscI TGG'CCA

Cuts at:      0     484     982    1410 
   Size:        484     498     428

  Fragments arranged by size: 

                 498     484     428
 
MslI CAynn'nnrTG

Cuts at:      0    1046    1279    1410 
   Size:       1046     233     131

  Fragments arranged by size: 

                1046     233     131
 
MspA1I CmG'CkG

Cuts at:      0      42     118     138     655     667     729    1410 
   Size:         42      76      20     517      12      62     681

  Fragments arranged by size: 

                 681     517      76      62      42      20      12
 
NcoI C'CATG_G

Cuts at:      0      11    1022    1355    1410 
   Size:         11    1011     333      55

  Fragments arranged by size: 

                1011     333      55      11
 
NdeI CA'TA_TG

Cuts at:      0     312    1410 
   Size:        312    1098
 
NspI r_CATG'y

Cuts at:      0      69     475    1410 
   Size:         69     406     935

  Fragments arranged by size: 

                 935     406      69
 
PasI CC'CwG_GG

Cuts at:      0     575    1410 
   Size:        575     835
 
PpuMI rG'GwC_Cy

Cuts at:      0     132     253     615     849    1410 
   Size:        132     121     362     234     561

  Fragments arranged by size: 

                 561     362     234     132     121
 
PspOMI G'GGCC_C

Cuts at:      0     809    1087    1380    1410 
   Size:        809     278     293      30

  Fragments arranged by size: 

                 809     293     278      30
 
PspXI vC'TCGA_Gb

Cuts at:      0      23    1410 
   Size:         23    1387
 
PstI C_TGCA'G

Cuts at:      0     281     446    1410 
   Size:        281     165     964

  Fragments arranged by size: 

                 964     281     165
 
PvuII CAG'CTG

Cuts at:      0     729    1410 
   Size:        729     681
 
RsrII CG'GwC_CG

Cuts at:      0     669    1410 
   Size:        669     741
 
SacII CC_GC'GG

Cuts at:      0      43    1410 
   Size:         43    1367
 
SalI G'TCGA_C

Cuts at:      0     406    1410 
   Size:        406    1004
 
SanDI GG'GwC_CC

Cuts at:      0     253    1410 
   Size:        253    1157
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1315    1410 
   Size:       1315      95
 
SbfI CC_TGCA'GG

Cuts at:      0     281     446    1410 
   Size:        281     165     964

  Fragments arranged by size: 

                 964     281     165
 
SfcI C'TryA_G

Cuts at:      0     277     442    1410 
   Size:        277     165     968

  Fragments arranged by size: 

                 968     277     165
 
SmlI C'TyrA_G

Cuts at:      0      23     628    1348    1410 
   Size:         23     605     720      62

  Fragments arranged by size: 

                 720     605      62      23
 
SphI G_CATG'C

Cuts at:      0      69    1410 
   Size:         69    1341
 
StyI C'CwwG_G

Cuts at:      0      11    1022    1355    1410 
   Size:         11    1011     333      55

  Fragments arranged by size: 

                1011     333      55      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     588    1410 
   Size:        588     822
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     743     775    1410 
   Size:        743      32     635

  Fragments arranged by size: 

                 743     635      32
 
XhoI C'TCGA_G

Cuts at:      0      23    1410 
   Size:         23    1387
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AlwNI     ApaI     AvaI     BaeI    BamHI
     BanI    BanII    BbvCI     BcgI     BglI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstXI    BstYI     BtgI     BtsI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I   HincII
     KpnI     MscI     MslI   MspA1I     NcoI     NdeI     NspI     PasI
    PpuMI   PspOMI    PspXI     PstI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI     SfcI     SmlI     SphI     StyI    TaqII
     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BbeI     BbsI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiWI
    BsmBI    BspEI    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI
     MfeI     MluI     MmeI     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI     PsrI     PvuI     SacI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI
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