Restriction map for pF1KE0109
##################################
# NO CUT ENZYMES (  187 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        KspI        MaeI        MaeII       MaeIII      
MamI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
Sau96I      SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AcsI          102  GAATTC               [GA]^AATT[CT]
AfaI           50  GTAC                 GT^AC
AlwI           39  GATCC                GGATC(4/5)
AlwNI         245  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          102  GAATTC               [GA]^AATT[CT]
AvaI          179  CCCGGG               C^[CT]CG[GA]G
BalI          146  TGGCCA               TGG^CCA
BclI          278  TGATCA               T^GATCA
BsmBI         302  CGTCTC               CGTCTC(1/5)
BsmFI         182  GGGAC                GGGAC(10/14)
BsoBI         179  CCCGGG               C^[CT]CG[GA]G
BstF5I        393  CATCC                GGATG(2/0)
Cac8I         250  GCTGGC               GC[ACGT]^[ACGT]GC
DdeI          353  CTGAG                C^T[ACGT]AG
DsaI          359  CCATGG               C^C[GA][CT]GG
EaeI          146  TGGCCA               [CT]^GGCC[GA]
EcoNI         348  CCTGTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         102  GAATTC               G^AATTC
EcoT14I       359  CCATGG               C^C[AT][AT]GG
FbaI          278  TGATCA               T^GATCA
FokI          393  CATCC                GGATG(9/13)
MluNI         146  TGGCCA               TGG^CCA
MscI          146  TGGCCA               TGG^CCA
MspA1I         42  CCGCTG               C[AC]G^C[GT]G
MwoI          214  GCTGGGGCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          359  CCATGG               C^CATGG
RsaI           50  GTAC                 GT^AC
SapI          318  GCTCTTC              GCTCTTC(1/4)
SfcI          123  CTACAG               C^T[GA][CT]AG
SmaI          179  CCCGGG               CCC^GGG
SmlI          307  CTCAAG               C^T[CT][GA]AG
StuI           67  AGGCCT               AGG^CCT
StyI          359  CCATGG               C^C[AT][AT]GG
Tsp509I       103  AATT                 ^AATT
XcmI          224  CCACAGTTGGTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         179  CCCGGG               C^CCGGG
XmaI          179  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AciI            8  CCGC                 CCGC(-3/-1)
               42  CCGC                
BpmI           29  CTCCAG               CTGGAG(16/14)
              139  CTCCAG              
BsiYI         135  CCCACTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              348  CCTGTCTGAGG         
BslI          135  CCCACTCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              348  CCTGTCTGAGG         
BsmAI         175  GTCTC                GTCTC(1/5)
              303  GTCTC               
BsrI           97  CCAGT                ACTGG(1/-1)
              167  CCAGT               
DpnII          39  GATC                 ^GATC
              279  GATC                
EarI          319  CTCTTC               CTCTTC(1/4)
              397  CTCTTC              
HapII          11  CCGG                 C^CGG
              180  CCGG                
HpaII          11  CCGG                 C^CGG
              180  CCGG                
Ksp632I       319  CTCTTC               CTCTTC(1/4)
              397  CTCTTC              
MboI           39  GATC                 ^GATC
              279  GATC                
MboII         320  TCTTC                GAAGA(8/7)
              398  TCTTC               
MspI           11  CCGG                 C^CGG
              180  CCGG                
NdeII          39  GATC                 ^GATC
              279  GATC                
NlaIII        360  CATG                 CATG^
              405  CATG                
NlaIV         259  GGAACC               GG[ACGT]^[ACGT]CC
              296  GGAACC              
Sau3AI         39  GATC                 ^GATC
              279  GATC                
TspRI          98  CAGTG                CA[GC]TG[ACGT][ACGT]^
              168  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        BbvI        BcnI        BsaJI       BssKI       
BstNI       EcoRII      Fnu4HI      HaeIII      ItaI        
MnlI        MvaI        NciI        ScrFI       TseI        
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