Restriction map for pF1KB9540
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        AspHI       
AspI        AvaI        AviII       BamHI       BbeI        
BbrPI       BciVI       BclI        BfrI        BglI        
BglII       BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KspI        MaeIII      MamI        MluI        
MroI        MspA1I      MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

Acc65I        881  GGTACC               G^GTACC
AhdI           43  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         994  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       273  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       881  GGTACC               G^GTACC
AspEI          43  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII          90  CCTAGG               C^CTAGG
BanI          881  GGTACC               G^G[CT][GA]CC
BbsI          631  GAAGAC               GAAGAC(2/6)
BbvCI         694  CCTCAGC              CCTCAGC(-5/-2)
BcnI          239  CCGGG                CC^[GC]GG
BlnI           90  CCTAGG               C^CTAGG
BpuAI         631  GAAGAC               GAAGAC(2/6)
BstYI         989  AGATCC               [GA]^GATC[CT]
Eam1105I       43  GACTTGATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         559  GATATC               GAT^ATC
FauI          166  GCGGG                CCCGC(4/6)
HapII         239  CCGG                 C^CGG
HpaII         239  CCGG                 C^CGG
KpnI          881  GGTACC               GGTAC^C
MfeI          419  CAATTG               C^AATTG
MflI          989  AGATCC               [GA]^GATC[CT]
MseI          442  TTAA                 T^TAA
MslI          660  CATCTGTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          239  CCGG                 C^CGG
MunI          419  CAATTG               C^AATTG
NciI          239  CCGGG                CC^[GC]GG
PleI          676  GAGTC                GAGTC(4/5)
SexAI         455  ACCAGGT              A^CC[AT]GGT
TfiI          973  GATTC                G^A[AT]TC
Tru9I         442  TTAA                 T^TAA
Tsp509I       420  AATT                 ^AATT
XhoII         989  AGATCC               [GA]^GATC[CT]
XmnI          273  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI          882  GTAC                 GT^AC
              996  GTAC                
BcgI          282  CGACTTGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              453  CGACCAGGTTGC        
BsaI           94  GGTCTC               GGTCTC(1/5)
              689  GGTCTC              
BseRI         171  CTCCTC               GAGGAG(10/8)
              692  CTCCTC              
Cac8I         166  GCGGGC               GC[ACGT]^[ACGT]GC
              183  GCTGGC              
DraII         503  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1046  GGGTCCC             
EcoO109I      503  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1046  GGGTCCC             
EcoT14I        90  CCTAGG               C^C[AT][AT]GG
              983  CCAAGG              
HinfI         676  GAGTC                G^A[ACGT]TC
              973  GATTC               
MaeII         343  ACGT                 A^CGT
              599  ACGT                
MwoI           14  GCTTCCTGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              595  GCTTACGTTGC         
PpuMI         503  GGGTCCT              [GA]G^G[AT]CC[CT]
             1046  GGGTCCC             
RsaI          882  GTAC                 GT^AC
              996  GTAC                
StyI           90  CCTAGG               C^C[AT][AT]GG
              983  CCAAGG              
TaqI           59  TCGA                 T^CGA
              281  TCGA                
TthHB8I        59  TCGA                 T^CGA
              281  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BalI        
BanII       BbvI        BfaI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cfr13I      DdeI        DpnII       EaeI        EarI        
EcoNI       EcoRII      Fnu4HI      FokI        HaeIII      
HphI        ItaI        Ksp632I     MaeI        MboI        
MboII       MluNI       MnlI        MscI        MvaI        
NdeII       NlaIII      NlaIV       Sau3AI      Sau96I      
ScrFI       SfaNI       TseI        TspRI       
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