Restriction map for pF1KE0337
 (Linear) (Six-base) MAPSORT of: KIEE0337.seq  Check: 3465  from: 1  to: 1527



KIEE0337.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1366    1527 
   Size:       1366     161
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     734    1527 
   Size:        734     793
 
AlwNI CAG_nnn'CTG

Cuts at:      0     480     531    1240    1527 
   Size:        480      51     709     287

  Fragments arranged by size: 

                 709     480     287      51
 
ApaI G_GGCC'C

Cuts at:      0     469    1044    1527 
   Size:        469     575     483

  Fragments arranged by size: 

                 575     483     469
 
ApaLI G'TGCA_C

Cuts at:      0     210    1527 
   Size:        210    1317
 
AvaI C'yCGr_G

Cuts at:      0     293     344     875    1439    1527 
   Size:        293      51     531     564      88

  Fragments arranged by size: 

                 564     531     293      88      51
 
BanI G'GyrC_C

Cuts at:      0      12     125     282     377    1527 
   Size:         12     113     157      95    1150

  Fragments arranged by size: 

                1150     157     113      95      12
 
BanII G_rGCy'C

Cuts at:      0     469    1044    1111    1213    1280    1527 
   Size:        469     575      67     102      67     247

  Fragments arranged by size: 

                 575     469     247     102      67      67
 
BbvCI CC'TCA_GC

Cuts at:      0    1078    1527 
   Size:       1078     449
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     866     900    1527 
   Size:        866      34     627

  Fragments arranged by size: 

                 866     627      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     582    1527 
   Size:        582     945
 
BglII A'GATC_T

Cuts at:      0    1463    1527 
   Size:       1463      64
 
Bme1580I G_kGCm'C

Cuts at:      0      15     128     214     287     469    1044    1099    1527 
   Size:         15     113      86      73     182     575      55     428

  Fragments arranged by size: 

                 575     428     182     113      86      73      55      15
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1392    1527 
   Size:       1392     135
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     370    1527 
   Size:        370    1157
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1239    1527 
   Size:        331     908     288

  Fragments arranged by size: 

                 908     331     288
 
Bpu10I CC'TnA_GC

Cuts at:      0    1078    1527 
   Size:       1078     449
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1375    1527 
   Size:       1375     152
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     853     898    1527 
   Size:        853      45     629

  Fragments arranged by size: 

                 853     629      45
 
BsaAI yAC'GTr

Cuts at:      0     195    1527 
   Size:        195    1332
 
BsaHI Gr'CG_yC

Cuts at:      0     275     638    1527 
   Size:        275     363     889

  Fragments arranged by size: 

                 889     363     275
 
BsaWI w'CCGG_w

Cuts at:      0     889    1527 
   Size:        889     638
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      16     328    1527 
   Size:         16     312    1199

  Fragments arranged by size: 

                1199     312      16
 
BseYI C'CCAG_C

Cuts at:      0     130     286     641    1036    1092    1329    1426    1494 
   Size:        130     156     355     395      56     237      97      68

Cuts at:   1494    1527 
   Size:         33

  Fragments arranged by size: 

                 395     355     237     156     130      97      68      56
                  33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1467    1527 
   Size:       1467      60
 
BsiEI CG_ry'CG

Cuts at:      0     243    1527 
   Size:        243    1284
 
BsiHKAI G_wGCw'C

Cuts at:      0     214    1527 
   Size:        214    1313
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     817    1527 
   Size:        817     710
 
Bsp1286I G_dGCh'C

Cuts at:      0      15     128     214     287     469    1044    1099    1111 
   Size:         15     113      86      73     182     575      55      12

Cuts at:   1111    1213    1280    1527 
   Size:        102      67     247

  Fragments arranged by size: 

                 575     247     182     113     102      86      73      67
                  55      15      12
 
BspHI T'CATG_A

Cuts at:      0    1272    1527 
   Size:       1272     255
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     493    1019    1334    1527 
   Size:        493     526     315     193

  Fragments arranged by size: 

                 526     493     315     193
 
BsrDI GCAATG_nn'

Cuts at:      0    1506    1527 
   Size:       1506      21
 
BsrFI r'CCGG_y

Cuts at:      0     238     242     367     495    1527 
   Size:        238       4     125     128    1032

  Fragments arranged by size: 

                1032     238     128     125       4
 
BssSI C'ACGA_G

Cuts at:      0     988    1527 
   Size:        988     539
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1240    1527 
   Size:       1240     287
 
BstEII G'GTnAC_C

Cuts at:      0     318    1527 
   Size:        318    1209
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1005    1434    1527 
   Size:       1005     429      93

  Fragments arranged by size: 

                1005     429      93
 
BstYI r'GATC_y

Cuts at:      0    1463    1515    1527 
   Size:       1463      52      12

  Fragments arranged by size: 

                1463      52      12
 
Bsu36I CC'TnA_GG

Cuts at:      0     395    1527 
   Size:        395    1132
 
BtgI C'CryG_G

Cuts at:      0       5      16     323     563     735    1155    1527 
   Size:          5      11     307     240     172     420     372

  Fragments arranged by size: 

                 420     372     307     240     172      11       5
 
BtsI GCAGTG_nn'

Cuts at:      0     839    1045    1229    1527 
   Size:        839     206     184     298

  Fragments arranged by size: 

                 839     298     206     184
 
ClaI AT'CG_AT

Cuts at:      0     432    1527 
   Size:        432    1095
 
EaeI y'GGCC_r

Cuts at:      0      50     240     326     365     497     551     632     958 
   Size:         50     190      86      39     132      54      81     326

Cuts at:    958     995    1340    1527 
   Size:         37     345     187

  Fragments arranged by size: 

                 345     326     190     187     132      86      81      54
                  50      39      37
 
EagI C'GGCC_G

Cuts at:      0     240    1527 
   Size:        240    1287
 
EarI CTCTTCn'nnn_

Cuts at:      0     815    1129    1527 
   Size:        815     314     398

  Fragments arranged by size: 

                 815     398     314
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      53    1527 
   Size:         53    1474
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331    1239    1366    1527 
   Size:        331     908     127     161

  Fragments arranged by size: 

                 908     331     161     127
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     313    1300    1527 
   Size:        313     987     227

  Fragments arranged by size: 

                 987     313     227
 
EcoO109I rG'GnC_Cy

Cuts at:      0     108     300     391     401     465     466     528     782 
   Size:        108     192      91      10      64       1      62     254

Cuts at:    782     804    1040    1041    1305    1449    1527 
   Size:         22     236       1     264     144      78

  Fragments arranged by size: 

                 264     254     236     192     144     108      91      78
                  64      62      22      10       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1212    1527 
   Size:       1212     315
 
HaeII r_GCGC'y

Cuts at:      0     648    1527 
   Size:        648     879
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1455    1487    1527 
   Size:       1455      32      40

  Fragments arranged by size: 

                1455      40      32
 
MscI TGG'CCA

Cuts at:      0     553     960     997    1527 
   Size:        553     407      37     530

  Fragments arranged by size: 

                 553     530     407      37
 
MslI CAynn'nnrTG

Cuts at:      0     361     868    1527 
   Size:        361     507     659

  Fragments arranged by size: 

                 659     507     361
 
MspA1I CmG'CkG

Cuts at:      0      18     159     737     775    1284    1386    1411    1527 
   Size:         18     141     578      38     509     102      25     116

  Fragments arranged by size: 

                 578     509     141     116     102      38      25      18
            
 
NaeI GCC'GGC

Cuts at:      0     244     369     497    1527 
   Size:        244     125     128    1030

  Fragments arranged by size: 

                1030     244     128     125
 
NcoI C'CATG_G

Cuts at:      0       5    1527 
   Size:          5    1522
 
NgoMIV G'CCGG_C

Cuts at:      0     242     367     495    1527 
   Size:        242     125     128    1032

  Fragments arranged by size: 

                1032     242     128     125
 
NspI r_CATG'y

Cuts at:      0     267    1527 
   Size:        267    1260
 
PasI CC'CwG_GG

Cuts at:      0    1206    1527 
   Size:       1206     321
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     531     993    1527 
   Size:        531     462     534

  Fragments arranged by size: 

                 534     531     462
 
PpuMI rG'GwC_Cy

Cuts at:      0     108     782     804    1305    1449    1527 
   Size:        108     674      22     501     144      78

  Fragments arranged by size: 

                 674     501     144     108      78      22
 
PshAI GACnn'nnGTC

Cuts at:      0     829    1527 
   Size:        829     698
 
PspOMI G'GGCC_C

Cuts at:      0     465    1040    1527 
   Size:        465     575     487

  Fragments arranged by size: 

                 575     487     465
 
PstI C_TGCA'G

Cuts at:      0     846    1236    1463    1527 
   Size:        846     390     227      64

  Fragments arranged by size: 

                 846     390     227      64
 
PvuII CAG'CTG

Cuts at:      0     159     775    1386    1411    1527 
   Size:        159     616     611      25     116

  Fragments arranged by size: 

                 616     611     159     116      25
 
SacII CC_GC'GG

Cuts at:      0      19     738    1527 
   Size:         19     719     789

  Fragments arranged by size: 

                 789     719      19
 
SanDI GG'GwC_CC

Cuts at:      0     782    1527 
   Size:        782     745
 
SfcI C'TryA_G

Cuts at:      0     842    1232    1459    1527 
   Size:        842     390     227      68

  Fragments arranged by size: 

                 842     390     227      68
 
SmaI CCC'GGG

Cuts at:      0     295    1441    1527 
   Size:        295    1146      86

  Fragments arranged by size: 

                1146     295      86
 
SmlI C'TyrA_G

Cuts at:      0    1390    1527 
   Size:       1390     137
 
StyI C'CwwG_G

Cuts at:      0       5     381     947    1061    1301    1527 
   Size:          5     376     566     114     240     226

  Fragments arranged by size: 

                 566     376     240     226     114       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     703    1219    1527 
   Size:        703     516     308

  Fragments arranged by size: 

                 703     516     308
 
TatI w'GTAC_w

Cuts at:      0     668    1184    1527 
   Size:        668     516     343

  Fragments arranged by size: 

                 668     516     343
 
Tth111I GACn'n_nGTC

Cuts at:      0    1150    1527 
   Size:       1150     377
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     147     546     561     804    1059    1527 
   Size:        147     399      15     243     255     468

  Fragments arranged by size: 

                 468     399     255     243     147      15
 
XmaI C'CCGG_G

Cuts at:      0     293    1439    1527 
   Size:        293    1146      88

  Fragments arranged by size: 

                1146     293      88
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII
    BbvCI     BcgI     BglI    BglII Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI
    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI
     BtsI     ClaI     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MscI     MslI   MspA1I     NaeI
     NcoI   NgoMIV     NspI     PasI    PflMI    PpuMI    PshAI   PspOMI
     PstI    PvuII    SacII    SanDI     SfcI     SmaI     SmlI     StyI
    TaqII     TatI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BbsI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmtI    BsaBI    BsaXI    BsiWI     BsmI    BspEI
    BsrBI    BsrGI   BssHII    BstBI  BstZ17I    BtgZI    CspCI     DraI
   DraIII     DrdI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NarI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI     XbaI     XhoI     XmnI     ZraI
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