Restriction map for pF1KB6880
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MunI        MwoI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        SwaI        
TfiI        TspRI       Tth111I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccII         503  CGCG                 CG^CG
AcsI          329  AAATTC               [GA]^AATT[CT]
AflIII         24  ACATGT               A^C[GA][CT]GT
AlwI          181  GATCC                GGATC(4/5)
AocI          448  CCTGAGG              CC^T[ACGT]AGG
ApoI          329  AAATTC               [GA]^AATT[CT]
AvaI          237  CCCGGG               C^[CT]CG[GA]G
BbvCI         558  CCTCAGC              CCTCAGC(-5/-2)
BglII         267  AGATCT               A^GATCT
BmyI          233  GTGCCC               G[AGT]GC[ACT]^C
BsaAI         510  CACGTA               [CT]AC^GT[GA]
BsoBI         237  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      233  GTGCCC               G[AGT]GC[ACT]^C
BspLU11I       24  ACATGT               A^CATGT
BstF5I         36  GGATG                GGATG(2/0)
BstNI         219  CCTGG                CC^[AT]GG
BstUI         503  CGCG                 CG^CG
Bsu36I        448  CCTGAGG              CC^T[ACGT]AGG
CfoI          502  GCGC                 GCG^C
DsaI          251  CCATGG               C^C[GA][CT]GG
Eco81I        448  CCTGAGG              CC^T[ACGT]AGG
EcoNI         279  CCTCAAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        219  CCTGG                ^CC[AT]GG
FokI           36  GGATG                GGATG(9/13)
HhaI          502  GCGC                 GCG^C
HinP1I        502  GCGC                 G^CGC
MboII         530  TCTTC                GAAGA(8/7)
MspA1I        561  CAGCTG               C[AC]G^C[GT]G
MvaI          219  CCTGG                CC^[AT]GG
MvnI          503  CGCG                 CG^CG
NcoI          251  CCATGG               C^CATGG
PstI          103  CTGCAG               CTGCA^G
PvuII         561  CAGCTG               CAG^CTG
SmaI          237  CCCGGG               CCC^GGG
SphI          494  GCATGC               GCATG^C
StuI          363  AGGCCT               AGG^CCT
TaqI          580  TCGA                 T^CGA
TthHB8I       580  TCGA                 T^CGA
XcmI          488  CCAAGGGCATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         237  CCCGGG               C^CCGGG
XmaI          237  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          141  GTAC                 GT^AC
              513  GTAC                
BpmI          470  CTCCAG               CTGGAG(16/14)
              263  CTGGAG              
BsiYI         279  CCTCAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              558  CCTCAGCTGGG         
BslI          279  CCTCAAAAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              558  CCTCAGCTGGG         
BsmFI         240  GGGAC                GGGAC(10/14)
               90  GTCCC               
BstYI         180  AGATCC               [GA]^GATC[CT]
              267  AGATCT              
DdeI          449  CTGAG                C^T[ACGT]AG
              559  CTCAG               
DpnII         181  GATC                 ^GATC
              268  GATC                
EcoT14I       251  CCATGG               C^C[AT][AT]GG
              488  CCAAGG              
HaeIII        364  GGCC                 GG^CC
              446  GGCC                
HapII         210  CCGG                 C^CGG
              238  CCGG                
HinfI         129  GAGTC                G^A[ACGT]TC
              323  GAGTC               
HpaII         210  CCGG                 C^CGG
              238  CCGG                
MaeII         374  ACGT                 A^CGT
              511  ACGT                
MaeIII        175  GTGAC                ^GT[ACGT]AC
              205  GTCAC               
MboI          181  GATC                 ^GATC
              268  GATC                
MflI          180  AGATCC               [GA]^GATC[CT]
              267  AGATCT              
MspI          210  CCGG                 C^CGG
              238  CCGG                
NdeII         181  GATC                 ^GATC
              268  GATC                
NspI           24  ACATGT               [GA]CATG^[CT]
              494  GCATGC              
PleI          129  GAGTC                GAGTC(4/5)
              323  GAGTC               
RsaI          141  GTAC                 GT^AC
              513  GTAC                
Sau3AI        181  GATC                 ^GATC
              268  GATC                
SfcI          103  CTGCAG               C^T[GA][CT]AG
              387  CTACAG              
StyI          251  CCATGG               C^C[AT][AT]GG
              488  CCAAGG              
Tsp45I        175  GTGAC                ^GT[GC]AC
              205  GTCAC               
XhoII         180  AGATCC               [GA]^GATC[CT]
              267  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   23 enzymes) #
#########################################

AluI        AvaII       BbvI        BcnI        BsaJI       
BseRI       BsrI        BssKI       Cac8I       Cfr13I      
Fnu4HI      HphI        ItaI        MnlI        MseI        
NciI        NlaIII      NlaIV       Sau96I      ScrFI       
Tru9I       TseI        Tsp509I     
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