Restriction map for pF1KB6880
 (Linear) (Six-base) MAPSORT of: KIBB6880.seq  Check: 513  from: 1  to: 603



KIBB6880.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AflIII A'CryG_T

Cuts at:      0      24     603 
   Size:         24     579
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     445     477     603 
   Size:        445      32     126

  Fragments arranged by size: 

                 445     126      32
 
ApoI r'AATT_y

Cuts at:      0     329     603 
   Size:        329     274
 
AvaI C'yCGr_G

Cuts at:      0     237     603 
   Size:        237     366
 
BbvCI CC'TCA_GC

Cuts at:      0     559     603 
   Size:        559      44
 
BglII A'GATC_T

Cuts at:      0     267     603 
   Size:        267     336
 
Bme1580I G_kGCm'C

Cuts at:      0     237     603 
   Size:        237     366
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     404     603 
   Size:        404     199
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     455     603 
   Size:        284     171     148

  Fragments arranged by size: 

                 284     171     148
 
Bpu10I CC'TnA_GC

Cuts at:      0     559     603 
   Size:        559      44
 
BsaAI yAC'GTr

Cuts at:      0     512     603 
   Size:        512      91
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     603 
   Size:        445      30     128

  Fragments arranged by size: 

                 445     128      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      46     466     493     603 
   Size:         46     420      27     110

  Fragments arranged by size: 

                 420     110      46      27
 
BseYI C'CCAG_C

Cuts at:      0     156     563     603 
   Size:        156     407      40

  Fragments arranged by size: 

                 407     156      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     237     603 
   Size:        237     366
 
BstYI r'GATC_y

Cuts at:      0     180     267     603 
   Size:        180      87     336

  Fragments arranged by size: 

                 336     180      87
 
Bsu36I CC'TnA_GG

Cuts at:      0     449     603 
   Size:        449     154
 
BtgI C'CryG_G

Cuts at:      0     251     603 
   Size:        251     352
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     455     603 
   Size:        284     171     148

  Fragments arranged by size: 

                 284     171     148
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     283     603 
   Size:        283     320
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     546     603 
   Size:        546      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     603 
   Size:        343      32     228

  Fragments arranged by size: 

                 343     228      32
 
MspA1I CmG'CkG

Cuts at:      0     563     603 
   Size:        563      40
 
NcoI C'CATG_G

Cuts at:      0     251     603 
   Size:        251     352
 
NspI r_CATG'y

Cuts at:      0      28     498     603 
   Size:         28     470     105

  Fragments arranged by size: 

                 470     105      28
 
PciI A'CATG_T

Cuts at:      0      24     603 
   Size:         24     579
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     603 
   Size:        445      32     126

  Fragments arranged by size: 

                 445     126      32
 
PsiI TTA'TAA

Cuts at:      0      71     603 
   Size:         71     532
 
PstI C_TGCA'G

Cuts at:      0     107     603 
   Size:        107     496
 
PvuII CAG'CTG

Cuts at:      0     563     603 
   Size:        563      40
 
SfcI C'TryA_G

Cuts at:      0     103     387     603 
   Size:        103     284     216

  Fragments arranged by size: 

                 284     216     103
 
SmaI CCC'GGG

Cuts at:      0     239     603 
   Size:        239     364
 
SphI G_CATG'C

Cuts at:      0     498     603 
   Size:        498     105
 
StuI AGG'CCT

Cuts at:      0     365     603 
   Size:        365     238
 
StyI C'CwwG_G

Cuts at:      0     251     488     603 
   Size:        251     237     115

  Fragments arranged by size: 

                 251     237     115
 
TatI w'GTAC_w

Cuts at:      0     140     603 
   Size:        140     463
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     508     540     603 
   Size:        508      32      63

  Fragments arranged by size: 

                 508      63      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     495     603 
   Size:        495     108
 
XmaI C'CCGG_G

Cuts at:      0     237     603 
   Size:        237     366
 

 Enzymes that do cut:

   AflIII     AloI     ApoI     AvaI    BbvCI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BsaAI    BsaXI    BseRI    BseYI Bsp1286I    BstYI
   Bsu36I     BtgI  Eco57MI    EcoNI     FalI    Hin4I   MspA1I     NcoI
     NspI     PciI     PpiI     PsiI     PstI    PvuII     SfcI     SmaI
     SphI     StuI     StyI     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmnI     ZraI
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